Western Blot (WB)
(Western blot analysis of extracts of HeLa cells, using CHCHD6 antibody.)
Rabbit anti-Human CHCHD6 Polyclonal Antibody | anti-CHCHD6 antibody
CHCHD6 Antibody
Gene Names
CHCHD6; CHCM1; Mic25; PPP1R23
Reactivity
Human
Applications
Western Blot, Immunohistochemistry
Purity
The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin.
Synonyms
CHCHD6; Polyclonal Antibody; CHCHD6 Antibody; CHCH6_HUMAN; Chchd6; CHCM1; Coiled coil helix cristae morphology 1; Coiled coil helix cristae morphology protein 1; Coiled-coil-helix-coiled-coil-helix domain-containing protein 6; mitochondrial; Mic25; MICOS complex subunit MIC25; PPP1R23; Protein phosphatase 1 regulatory subunit 23; anti-CHCHD6 antibody
Host
Rabbit
Reactivity
Human
Clonality
Polyclonal
Isotype
IgG
Specificity
CHCHD6 antibody detects endogenous levels of CHCHD6.
Purity/Purification
The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin.
Form/Format
Liquid
Phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Concentration
1mg/ml (varies by lot)
Sequence Length
236
Applicable Applications for anti-CHCHD6 antibody
Western Blot (WB), Immunohistochemistry (IHC)
Application Notes
WB: 1:500-1:2000
IHC: 1:50-1:200
IHC: 1:50-1:200
Immunogen
A synthesized peptide derived from human CHCHD6.
Subcellular Location
Mitochondrion
Tissue Specificity
Down-regulated following genotoxic stress.
Predicted Cross Reactivity
Pig, Bovine, Rabbit
Similarity
Pig (89%), Bovine (100%), Rabbit (100%)
Conjugation
Unconjugated
Preparation and Storage
Store at -20 degree C. Stable for 12 months from date of receipt.
Western Blot (WB)
(Western blot analysis of extracts of HeLa cells, using CHCHD6 antibody.)
Western Blot (WB)
(Western blot analysis of extracts of HeLa cells, using CHCHD6 antibody.)
Western Blot (WB)
(Western blot analysis of extracts from rat brain, using CHCHD6 antibody. Lane 1 was treated with the blocking peptide.)
Western Blot (WB)
(Western blot analysis of extracts from rat brain, using CHCHD6 antibody. Lane 1 was treated with the blocking peptide.)
Immunohistochemistry (IHC)
(MBS9603483 at 1/100 staining Human lymph cancer tissue by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the antibody for 1.5 hours at 22 degree C. An HRP conjugated goat anti-rabbit antibody was used as the secondary.)
Immunohistochemistry (IHC)
(MBS9603483 at 1/100 staining Human lymph cancer tissue by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the antibody for 1.5 hours at 22 degree C. An HRP conjugated goat anti-rabbit antibody was used as the secondary.)
Related Product Information for anti-CHCHD6 antibody
Function: Component of the MICOS complex, a large protein complex of the mitochondrial inner membrane that plays crucial roles in the maintenance of crista junctions, inner membrane architecture, and formation of contact sites to the outer membrane.
Subunit Structure: Component of the mitochondrial contact site and cristae organizing system (MICOS) complex, composed of at least MINOS1/MIC10, CHCHD3/MIC19, CHCHD6/MIC25, APOOL/MIC27, IMMT/MIC60, APOO/MIC23/MIC26 and QIL1/MIC13. This complex was also known under the names MINOS or MitOS complex. The MICOS complex associates with mitochondrial outer membrane proteins SAMM50, MTX1 and MTX2 (together described as components of the mitochondrial outer membrane sorting assembly machinery (SAM) complex) and DNAJC11, mitochondrial inner membrane protein TMEM11 and with HSPA9 (PubMed:17624330, PubMed:22228767, PubMed:25781180, PubMed:25997101). The MICOS and SAM complexes together with DNAJC11 are part of a large protein complex spanning both membranes termed the mitochondrial intermembrane space bridging (MIB) complex. Interacts with DISC1 (PubMed:22228767). Interacts with DISC1 (PubMed:22228767). Interacts with IMMT/MIC60 (PubMed:22228767, PubMed:25997101).
Similarity: Belongs to the MICOS complex subunit Mic19 family. Metazoan Mic25 subfamily.
Subunit Structure: Component of the mitochondrial contact site and cristae organizing system (MICOS) complex, composed of at least MINOS1/MIC10, CHCHD3/MIC19, CHCHD6/MIC25, APOOL/MIC27, IMMT/MIC60, APOO/MIC23/MIC26 and QIL1/MIC13. This complex was also known under the names MINOS or MitOS complex. The MICOS complex associates with mitochondrial outer membrane proteins SAMM50, MTX1 and MTX2 (together described as components of the mitochondrial outer membrane sorting assembly machinery (SAM) complex) and DNAJC11, mitochondrial inner membrane protein TMEM11 and with HSPA9 (PubMed:17624330, PubMed:22228767, PubMed:25781180, PubMed:25997101). The MICOS and SAM complexes together with DNAJC11 are part of a large protein complex spanning both membranes termed the mitochondrial intermembrane space bridging (MIB) complex. Interacts with DISC1 (PubMed:22228767). Interacts with DISC1 (PubMed:22228767). Interacts with IMMT/MIC60 (PubMed:22228767, PubMed:25997101).
Similarity: Belongs to the MICOS complex subunit Mic19 family. Metazoan Mic25 subfamily.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
Observed: 26-29 kDa
Predicted: 27 kDa
Predicted: 27 kDa
NCBI Official Full Name
MICOS complex subunit MIC25 isoform 1
NCBI Official Synonym Full Names
coiled-coil-helix-coiled-coil-helix domain containing 6
NCBI Official Symbol
CHCHD6
NCBI Official Synonym Symbols
CHCM1; Mic25; PPP1R23
NCBI Protein Information
MICOS complex subunit MIC25
UniProt Protein Name
MICOS complex subunit MIC25
Protein Family
UniProt Gene Name
CHCHD6
UniProt Synonym Gene Names
CHCM1; MIC25
Uniprot Description
Component of the MICOS complex, a large protein complex of the mitochondrial inner membrane that plays crucial roles in the maintenance of crista junctions, inner membrane architecture, and formation of contact sites to the outer membrane.
Research Articles on CHCHD6
Similar Products
Product Notes
The CHCHD6 chchd6 (Catalog #AAA9603483) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The CHCHD6 Antibody reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's CHCHD6 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunohistochemistry (IHC). WB: 1:500-1:2000 IHC: 1:50-1:200. Researchers should empirically determine the suitability of the CHCHD6 chchd6 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "CHCHD6, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
If you are ready to order, navigate to Shopping Cart and get ready to checkout.
