Western Blot (WB)
(Western blot analysis of extracts from various samples, using TOM40 Antibody. Lane 1: B16F10 cells, treated with blocking peptide; Lane 2: B16F10 cells;
Lane 3: Mouse heart.)
Rabbit TOM40 Polyclonal Antibody | anti-TOM40 antibody
TOM40 Antibody
Gene Names
TOMM40; TOM40; PEREC1; C19orf1; PER-EC1; D19S1177E
Reactivity
Human, Mouse, Rat
Applications
Western Blot, Immunohistochemistry, ELISA
Purity
Peptide affinity purification
Synonyms
TOM40; Polyclonal Antibody; TOM40 Antibody; C19orf1; D19S1177E; Haymaker protein; Mitochondrial import receptor subunit TOM40 homolog; Mitochondrial outer membrane protein; Mitochondrial outer membrane protein TOM40; p38.5; PER EC1; PEREC1; Probable mitochondrial import receptor subunit TOM40 homolog; Protein Haymaker; TOM40_HUMAN; TOMM40; Translocase of outer membrane 40 kDa subunit homolog; Translocase of outer mitochondrial membrane 40; Translocase of outer mitochondrial membrane 40 homolog (yeast); Translocase of outer mitochondrial membrane 40 homolog; yeast; homolog of; anti-TOM40 antibody
Host
Rabbit
Reactivity
Human, Mouse, Rat
Clonality
Polyclonal
Isotype
IgG
Specificity
TOM40 antibody detects endogenous levels of TOM40.
Purity/Purification
Peptide affinity purification
Form/Format
Phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Concentration
1mg/ml (varies by lot)
Sequence Length
361
Applicable Applications for anti-TOM40 antibody
Western Blot (WB), Immunohistochemistry (IHC), ELISA (peptide)
Application Notes
WB: 1:500-1:2000
IHC: 1:50-1:200
ELISA(peptide): 1:20000-1:40000
*The optimal dilutions should be determined by the end user.
IMPORTANT: For western blots, incubate membrane with diluted antibody in 5% w/v milk , 1X TBS, 0.1% Tween®20 at 4°C with gentle shaking, overnight.
IHC: 1:50-1:200
ELISA(peptide): 1:20000-1:40000
*The optimal dilutions should be determined by the end user.
IMPORTANT: For western blots, incubate membrane with diluted antibody in 5% w/v milk , 1X TBS, 0.1% Tween®20 at 4°C with gentle shaking, overnight.
Immunogen
A synthesized peptide derived from human TOM40, corresponding to a region within the internal amino acids.
Preparation and Storage
Store at -20 degree C. Stable for 12 months from date of receipt.
Western Blot (WB)
(Western blot analysis of extracts from various samples, using TOM40 Antibody. Lane 1: B16F10 cells, treated with blocking peptide; Lane 2: B16F10 cells; Lane 3: Mouse heart.)
Western Blot (WB)
(Western blot analysis of extracts from various samples, using TOM40 Antibody. Lane 1: B16F10 cells, treated with blocking peptide; Lane 2: B16F10 cells;
Lane 3: Mouse heart.)
Immunohistochemistry (IHC)
(MBS9603467 at 1/100 staining Rat kidney tissue by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the primary antibody at 4°C overnight. An HRP conjugated anti-Rabbit antibody was used as the secondary antibody.)
Immunohistochemistry (IHC)
(MBS9603467 at 1/100 staining Rat kidney tissue by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the primary antibody at 4°C overnight. An HRP conjugated anti-Rabbit antibody was used as the secondary antibody.)
Related Product Information for anti-TOM40 antibody
Channel-forming protein essential for import of protein precursors into mitochondria.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
38 kDa
NCBI Official Full Name
mitochondrial import receptor subunit TOM40 homolog
NCBI Official Synonym Full Names
translocase of outer mitochondrial membrane 40
NCBI Official Symbol
TOMM40
NCBI Official Synonym Symbols
TOM40; PEREC1; C19orf1; PER-EC1; D19S1177E
NCBI Protein Information
mitochondrial import receptor subunit TOM40 homolog
UniProt Protein Name
Mitochondrial import receptor subunit TOM40 homolog
Protein Family
UniProt Gene Name
TOMM40
UniProt Synonym Gene Names
C19orf1; PEREC1; TOM40
NCBI Description
The protein encoded by this gene is localized in the outer membrane of the mitochondria. It is the channel-forming subunit of the translocase of the mitochondrial outer membrane (TOM) complex that is essential for import of protein precursors into mitochondria. Alternatively spliced transcript variants have been found for this gene. [provided by RefSeq, Aug 2015]
Uniprot Description
Channel-forming protein essential for import of protein precursors into mitochondria.
Research Articles on TOM40
Similar Products
Product Notes
The TOM40 tomm40 (Catalog #AAA9603467) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The TOM40 Antibody reacts with Human, Mouse, Rat and may cross-react with other species as described in the data sheet. AAA Biotech's TOM40 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunohistochemistry (IHC), ELISA (peptide). WB: 1:500-1:2000 IHC: 1:50-1:200 ELISA(peptide): 1:20000-1:40000 *The optimal dilutions should be determined by the end user. IMPORTANT: For western blots, incubate membrane with diluted antibody in 5% w/v milk, 1X TBS, 0.1% Tween®20 at 4°C with gentle shaking, overnight. Researchers should empirically determine the suitability of the TOM40 tomm40 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "TOM40, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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