Immunohistochemistry (IHC)
(MBS9603275 at 1/100 staining Human lymph tissue by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the antibody for 1.5 hours at 22 degree C. An HRP conjugated goat anti-rabbit antibody was used as the secondary.)
Rabbit IFT57 Polyclonal Antibody | anti-IFT57 antibody
IFT57 Antibody
Gene Names
IFT57; HIPPI; OFD18; MHS4R2; ESRRBL1
Reactivity
Human, Mouse, Rat, Dog
Applications
Western Blot, Immunohistochemistry
Purity
The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin.
Synonyms
IFT57; Polyclonal Antibody; IFT57 Antibody; CDNA FLJ10147 fis clone HEMBA1003369; Dermal papilla derived protein 8; Dermal papilla-derived protein 8; DERP 8; DERP8; Esrrbl 1; Esrrbl1; ESRRBL1 protein; Estrogen related receptor beta like 1; Estrogen related receptor beta like protein 1; Estrogen-related receptor beta-like protein 1; FLJ10147; Hip1 Interacting protein; HIP1 interacting protein HIPPI; Hip1 protein interactor; HIP1-interacting protein; HIPPI; Huntingtin interacting protein 1 interacting protein; Huntingtin interacting protein 1 protein interactor; IFT 57; ift57; IFT57_HUMAN; Intraflagellar transport 57 homolog (Chlamydomonas); Intraflagellar transport 57 homolog; Intraflagellar transport protein 57 homolog; MHS4R2; Vestrogen related receptor beta like 1; anti-IFT57 antibody
Host
Rabbit
Reactivity
Human, Mouse, Rat, Dog
Clonality
Polyclonal
Isotype
IgG
Specificity
IFT57 antibody detects endogenous levels of IFT57.
Purity/Purification
The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin.
Form/Format
Liquid
Phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Concentration
1mg/ml (varies by lot)
Sequence Length
429
Applicable Applications for anti-IFT57 antibody
Western Blot (WB), Immunohistochemistry (IHC)
Application Notes
WB: 1:500-1:2000
IHC: 1:50-1:200
IHC: 1:50-1:200
Immunogen
A synthesized peptide derived from human IFT57.
Subcellular Location
Cytoplasm > Cytoskeleton > Cilium Basal Body. Concentrates within the inner segment of cilia.
Tissue Specificity
Present in many tissues such as brain, thymus, lymph node, lung, liver, skin and kidney (at protein level).
Predicted Cross Reactivity
Pig, Zebrafish, Bovine, Horse, Sheep, Rabbit, Dog, Chicken, Xenopus
Similarity
Pig (100%), Zebrafish (92%), Bovine (92%), Horse (100%), Sheep (92%), Rabbit (100%), Dog (100%), Chicken (92%), Xenopus (100%)
Conjugation
Unconjugated
Preparation and Storage
Store at -20 degree C. Stable for 12 months from date of receipt.
Immunohistochemistry (IHC)
(MBS9603275 at 1/100 staining Human lymph tissue by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the antibody for 1.5 hours at 22 degree C. An HRP conjugated goat anti-rabbit antibody was used as the secondary.)
Immunohistochemistry (IHC)
(MBS9603275 at 1/100 staining Human lymph tissue by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the antibody for 1.5 hours at 22 degree C. An HRP conjugated goat anti-rabbit antibody was used as the secondary.)
Western Blot (WB)
(Western blot analysis of extracts of HEK-293 cells, using IFT57 antibody.)
Western Blot (WB)
(Western blot analysis of extracts of HEK-293 cells, using IFT57 antibody.)
Related Product Information for anti-IFT57 antibody
Function: Required for the formation of cilia. Plays an indirect role in sonic hedgehog signaling, cilia being required for all activity of the hedgehog pathway (By similarity). Has pro-apoptotic function via its interaction with HIP1, leading to recruit caspase-8 (CASP8) and trigger apoptosis. Has the ability to bind DNA sequence motif 5'-AAAGACATG-3' present in the promoter of caspase genes such as CASP1, CASP8 and CASP10, suggesting that it may act as a transcription regulator; however the relevance of such function remains unclear.
Subunit Structure: Component of the IFT complex B, at least composed of IFT20, IFT22, HSPB11/IFT25, IFT27, IFT46, IFT52, TRAF3IP1/IFT54, IFT57, IFT74, IFT80, IFT81, and IFT88. Interacts with IFT20 and IFT88. Interacts with BLOC1S2 and RYBP. Interacts with HOMER1, possibly preventing pro-apoptotic effects of IFT57 (By similarity). Interacts with HIP1. In normal conditions, it poorly interacts with HIP1, HIP1 being strongly associated with HTT. However, in mutant HTT proteins with a long poly-Gln region, interaction between HTT and HIP1 is inhibited, promoting the interaction between HIP1 and IFT57, leading to apoptosis. Interacts with BFAR. Interacts with chicken anemia virus protein apoptin. Interacts with TTC25 (PubMed:25860617).
Similarity: The pseudo DED region (pDED) meadiates the interaction with HIP1. Belongs to the IFT57 family.
Subunit Structure: Component of the IFT complex B, at least composed of IFT20, IFT22, HSPB11/IFT25, IFT27, IFT46, IFT52, TRAF3IP1/IFT54, IFT57, IFT74, IFT80, IFT81, and IFT88. Interacts with IFT20 and IFT88. Interacts with BLOC1S2 and RYBP. Interacts with HOMER1, possibly preventing pro-apoptotic effects of IFT57 (By similarity). Interacts with HIP1. In normal conditions, it poorly interacts with HIP1, HIP1 being strongly associated with HTT. However, in mutant HTT proteins with a long poly-Gln region, interaction between HTT and HIP1 is inhibited, promoting the interaction between HIP1 and IFT57, leading to apoptosis. Interacts with BFAR. Interacts with chicken anemia virus protein apoptin. Interacts with TTC25 (PubMed:25860617).
Similarity: The pseudo DED region (pDED) meadiates the interaction with HIP1. Belongs to the IFT57 family.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
Observed: 49 kDa
Predicted: 50 kDa
Predicted: 50 kDa
NCBI Official Full Name
intraflagellar transport protein 57 homolog
NCBI Official Synonym Full Names
intraflagellar transport 57
NCBI Official Symbol
IFT57
NCBI Official Synonym Symbols
HIPPI; OFD18; MHS4R2; ESRRBL1
NCBI Protein Information
intraflagellar transport protein 57 homolog
UniProt Protein Name
Intraflagellar transport protein 57 homolog
Protein Family
UniProt Gene Name
IFT57
UniProt Synonym Gene Names
DERP8; ESRRBL1; HIPPI
Uniprot Description
Required for the formation of cilia. Plays an indirect role in sonic hedgehog signaling, cilia being required for all activity of the hedgehog pathway (). Has pro-apoptotic function via its interaction with HIP1, leading to recruit caspase-8 (CASP8) and trigger apoptosis. Has the ability to bind DNA sequence motif 5'-AAAGACATG-3' present in the promoter of caspase genes such as CASP1, CASP8 and CASP10, suggesting that it may act as a transcription regulator; however the relevance of such function remains unclear.
Research Articles on IFT57
Similar Products
Product Notes
The IFT57 ift57 (Catalog #AAA9603275) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The IFT57 Antibody reacts with Human, Mouse, Rat, Dog and may cross-react with other species as described in the data sheet. AAA Biotech's IFT57 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunohistochemistry (IHC). WB: 1:500-1:2000 IHC: 1:50-1:200. Researchers should empirically determine the suitability of the IFT57 ift57 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "IFT57, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
If you are ready to order, navigate to Shopping Cart and get ready to checkout.
