Western Blot (WB)
(Western blot analysis of VPS54 using HepG2 whole cell lysates)
Rabbit anti-Human VPS54 Polyclonal Antibody | anti-VPS54 antibody
VPS54 Antibody
Gene Names
VPS54; WR; HCC8; SLP-8p; VPS54L; hVps54L; PPP1R164
Reactivity
Human
Applications
Western Blot, ELISA
Purity
The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin.
Synonyms
VPS54; Polyclonal Antibody; VPS54 Antibody; HCC8; Hepatocellular carcinoma protein 8; hVps54L; PPP1R164; Protein phosphatase 1 regulatory subunit 164; SLP 8p; Tumor antigen HOM-HCC-8; Tumor antigen SLP 8p; Tumor antigen SLP-8p; Vacuolar protein sorting 54 homolog (S. cerevisiae); Vacuolar protein sorting 54; S. cerevisiae; homolog of; Vacuolar protein sorting associated protein 54; VPS54 GARP complex subunit; VPS54-like; VPS54L; Wobbler; mouse; WR; anti-VPS54 antibody
Host
Rabbit
Reactivity
Human
Clonality
Polyclonal
Isotype
IgG
Specificity
VPS54 antibody detects endogenous levels of total VPS54
Purity/Purification
The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin.
Form/Format
Liquid
Phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Concentration
1mg/ml (varies by lot)
Sequence Length
965
Applicable Applications for anti-VPS54 antibody
Western Blot (WB), ELISA (EIA)
Application Notes
WB: 1:1000-3000
Immunogen
A synthesized peptide derived from human VPS54
Subcellular Location
Golgi Apparatus
Predicted Cross Reactivity
Pig, Bovine, Horse, Sheep, Rabbit, Dog, Chicken
Similarity
Pig (100%), Bovine (100%), Horse (100%), Sheep (100%), Rabbit (100%), Dog (100%), Chicken (100%)
Conjugation
Unconjugated
Preparation and Storage
Store at -20 degree C. Stable for 12 months from date of receipt.
Western Blot (WB)
(Western blot analysis of VPS54 using HepG2 whole cell lysates)
Western Blot (WB)
(Western blot analysis of VPS54 using HepG2 whole cell lysates)
Related Product Information for anti-VPS54 antibody
Function: Acts as component of the GARP complex that is involved in retrograde transport from early and late endosomes to the trans-Golgi network (TGN). The GARP complex is required for the maintenance of the cycling of mannose 6-phosphate receptors between the TGN and endosomes, this cycling is necessary for proper lysosomal sorting of acid hydrolases such as CTSD (PubMed:18367545). Within the GARP complex, required to tether the complex to the TGN. Not involved in endocytic recycling (PubMed:25799061).
Subunit Structure: Component of the Golgi-associated retrograde protein (GARP) complex, also called VFT (VPS fifty-three) complex, composed of VPS51, VPS52, VPS53 and VPS54.
Similarity: Belongs to the VPS54 family.
Subunit Structure: Component of the Golgi-associated retrograde protein (GARP) complex, also called VFT (VPS fifty-three) complex, composed of VPS51, VPS52, VPS53 and VPS54.
Similarity: Belongs to the VPS54 family.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
Observed: 111 kDa
Predicted: 111 kDa
Predicted: 111 kDa
NCBI Official Full Name
vacuolar protein sorting-associated protein 54 isoform 2
NCBI Official Synonym Full Names
VPS54, GARP complex subunit
NCBI Official Symbol
VPS54
NCBI Official Synonym Symbols
WR; HCC8; SLP-8p; VPS54L; hVps54L; PPP1R164
NCBI Protein Information
vacuolar protein sorting-associated protein 54
UniProt Protein Name
Vacuolar protein sorting-associated protein 54
UniProt Gene Name
VPS54
UniProt Synonym Gene Names
HCC8
NCBI Description
This gene encodes for a protein that in yeast forms part of a trimeric vacuolar-protein-sorting complex that is required for retrograde transport of proteins from prevacuoles to the late Golgi compartment. As in yeast, mammalian Vps54 proteins contain a coiled-coil region and dileucine motifs. Alternative splicing results in multiple transcript variants encoding different isoforms [provided by RefSeq, Jul 2008]
Uniprot Description
Acts as component of the GARP complex that is involved in retrograde transport from early and late endosomes to the trans-Golgi network (TGN). The GARP complex is required for the maintenance of the cycling of mannose 6-phosphate receptors between the TGN and endosomes, this cycling is necessary for proper lysosomal sorting of acid hydrolases such as CTSD (PubMed:18367545). Within the GARP complex, required to tether the complex to the TGN. Not involved in endocytic recycling (PubMed:25799061).
Research Articles on VPS54
Similar Products
Product Notes
The VPS54 vps54 (Catalog #AAA9602942) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The VPS54 Antibody reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's VPS54 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), ELISA (EIA). WB: 1:1000-3000. Researchers should empirically determine the suitability of the VPS54 vps54 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "VPS54, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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