IL-12 active protein

Human IL-12 (IL-12A & IL-12B) Protein, His Tag

Cat. Num. AAA9718731

• Gene Names: IL12A; P35; CLMF; NFSK; NKSF1; IL-12A
• Purity: >95% as determined by SDS-PAGE
• Synonyms: IL-12; Human IL-12 (IL-12A & IL-12B) Protein; His Tag; Recombinant Human IL- 12 (IL12A & IL12B Heterodimer) Protein (Interleukin-12 subunit alpha & Interleukin-12 subunit beta) expressed in Human Cells; CLMF; CLMF2; IL-12A; IL-12B; IL12; IMD28; IMD29; NFSK; NKSF; NKSF1; NKSF2; P35; Interleukin-12 subunit alpha & Interleukin-12 subunit beta; IL12A & IL12B; IL-12 active protein

• Host: Human Cells
• Purity/Purification: >95% as determined by SDS-PAGE
• Form/Format: Lyophilized from sterile PBS, pH 7.4
• Sequence: Amino acid sequence derived from p35 subunit of human IL-12, termed as IL12A (NP_000873.2) (Met 1-Ser 219) was fused with a polyhistidine tag at the C-terminus, constructed the plasmid 1; Amino acid sequence derived from p40 subunit of human IL-12, termed as IL12B (NP_002178.2) (Met 1-Ser 328) was fused with a polyhistidine tag at the C-terminus, constructed the plasmid 2. The two plasmids were co-expressed and the heterodimer was purified.
• Sequence Length: 253

• Species: Human
• Endotoxin: The Endotoxin level is less than 1.0 EU per mug of the protein as determined by the LAL method
• Protein Length: The recombinant IL12 heterodimer of human IL12A/IL12B comprises 525 (208 + 317) amino acids and has a calculated molecular mass of 60.2 (24 + 36.2) kDa. The apparent molecular mass of rh IL12 heterodimer is approximately 40 & 43 kDa respectively in SDS-PAGE under reducing conditions.
• Activity: Measured by its ability to bind biotinylated recombinant human IL12RB1 in a functional ELISA. Measured in a cell proliferation assay using Anti-CD28 and Anti-CD3 stimulated PBMC. The ED50 for this effect is typically 0.1-0.6 ng/mL. 2. Measured in a cell proliferation assay using Anti-CD28 and Anti-CD3 stimulated PBMC. The ED50 for this effect is typically 0.1-0.6 ng/mL.
• Usage Notes: Always centrifuge tubes before opening. It is recommended to reconstitute the lyophilized Human IL- 12 (IL12A & IL12B Heterodimer) protein in sterile ddH2O not less than 100ug/ml, which can then be further diluted to other aqueous solutions.
• Preparation and Storage: Item should be stored desiccated below -20 degree C. Upon reconstitution, the protein should be stored at 4 degree C between 2-7 days and for future use below -20 degree C. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA). Please prevent freeze-thaw cycles.

SDS-Page

(Fig.SDS-PAGE analysis of Human IL- 12 (IL-12A & IL-12B) protein.)

Related Product Information for IL-12 active protein

IL-12 is composed of a bundle of four alpha helices. It is a heterodimeric cytokine encoded by two separate genes, IL-12A (p35) and IL-12B (p40). The active heterodimer (referred to as 'p70'), and a homodimer of p40 are formed following protein synthesis.IL-12 is involved in the differentiation of naive T cells into Th1 cells. It is known as a T cell-stimulating factor, which can stimulate the growth and function of T cells. It stimulates the production of interferon-gamma (IFN-gamma) and tumor necrosis factor-alpha (TNF-alpha) from T cells and natural killer (NK) cells, and reduces IL-4 mediated suppression of IFN-gamma. T cells that produce IL-12 have a coreceptor, CD30, which is associated with IL-12 activity. IL-12 plays an important role in the activities of natural killer cells and T lymphocytes. IL-12 mediates enhancement of the cytotoxic activity of NK cells and CD8+ cytotoxic T lymphocytes. There also seems to be a link between IL-2 and the signal transduction of IL-12 in NK cells. IL-2 stimulates the expression of two IL-12 receptors, IL-12R-beta1 and IL-12R-beta2, maintaining the expression of a critical protein involved in IL-12 signaling in NK cells. Enhanced functional response is demonstrated by IFN-gamma production and killing of target cells.

NCBI and Uniprot Product Information

• NCBI GI #: 24430219
• NCBI GeneID: 3592
• NCBI Accession #: NP_000873.2; NP_002178.2
• NCBI GenBank Nucleotide #: NP_000873.2
• UniProt Accession #: P29459
• NCBI Official Full Name: interleukin-12 subunit alpha isoform 1
• NCBI Official Synonym Full Names: interleukin 12A
• NCBI Official Symbol: IL12A
• NCBI Official Synonym Symbols: P35; CLMF; NFSK; NKSF1; IL-12A
• NCBI Protein Information: interleukin-12 subunit alpha

NCBI Description

This gene encodes a subunit of a cytokine that acts on T and natural killer cells, and has a broad array of biological activities. The cytokine is a disulfide-linked heterodimer composed of the 35-kD subunit encoded by this gene, and a 40-kD subunit that is a member of the cytokine receptor family. This cytokine is required for the T-cell-independent induction of interferon (IFN)-gamma, and is important for the differentiation of both Th1 and Th2 cells. The responses of lymphocytes to this cytokine are mediated by the activator of transcription protein STAT4. Nitric oxide synthase 2A (NOS2A/NOS2) is found to be required for the signaling process of this cytokine in innate immunity. [provided by RefSeq, Jul 2008]