Rabbit anti-Human ATOH8 Polyclonal Antibody | anti-ATOH8 antibody

ATOH8 Antibody

Cat. Num. AAA9602906

• Gene Names: ATOH8; HATH6; bHLHa21
• Reactivity: Human
• Applications: Western Blot, ELISA
• Purity: The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin.
• Synonyms: ATOH8; Polyclonal Antibody; ATOH8 Antibody; 4933425C05Rik; ATOH8_HUMAN; Atonal homolog 6; atonal homolog 8 (Drosophila); Atonal homolog 8; Basic helix loop helix transcription factor 6; bHLHa21; Class A basic helix-loop-helix protein 21; FLJ14708; FLJ38730; hATH6; Helix loop helix protein mATH-6; Helix-loop-helix protein hATH-6; Math6; Okadin; Protein atonal homolog 8; RGD1561512; anti-ATOH8 antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: ATOH8 antibody detects endogenous levels of total ATOH8
• Purity/Purification: The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin.
• Form/Format: Liquid; Phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
• Concentration: 1mg/ml (varies by lot)
• Sequence Length: 321

• Applicable Applications for anti-ATOH8 antibody: Western Blot (WB), ELISA (EIA)
• Application Notes: WB: 1:1000-3000
• Immunogen: A synthesized peptide derived from human ATOH8
• Subcellular Location: Nucleus.
• Tissue Specificity: Expressed in lung, liver, kidney, heart and pancreas. Expressed in endothel of umbilical vessels.
• Predicted Cross Reactivity: Pig, Bovine, Sheep, Rabbit, Dog, Chicken, Xenopus
• Similarity: Pig (100%), Bovine (100%), Sheep (100%), Rabbit (100%), Dog (100%), Chicken (92%), Xenopus (92%)
• Conjugation: Unconjugated
• Preparation and Storage: Store at -20 degree C. Stable for 12 months from date of receipt.

Western Blot (WB)

(Western blot analysis of ATOH8 using 293 whole cell lysates)

Related Product Information for anti-ATOH8 antibody

Function: Transcription factor that binds a palindromic (canonical) core consensus DNA sequence 5'-CANNTG-3' known as an E-box element, possibly as a heterodimer with other bHLH proteins (PubMed:24236640). Regulates endothelial cell proliferation, migration and tube-like structures formation (PubMed:24463812). Modulates endothelial cell differentiation through NOS3 (PubMed:24463812). May be implicated in specification and differentiation of neuronal cell lineages in the brain (By similarity). May participate in kidney development and may be involved in podocyte differentiation (By similarity). During early embryonic development is involved in tissue-specific differentiation processes that are dependent on class II bHLH factors and namely modulates the differentiation program initiated by the pro-endocrine factor NEUROG3 (By similarity). During myogenesis, may play a role during the transition of myoblasts from the proliferative phase to the differentiation phase (By similarity). Positively regulates HAMP transcription in two ways, firstly by acting directly on the HAMP promoter via E-boxes binding and indirectly through increased phosphorylation of SMAD protein complex (PubMed:24236640). Repress NEUROG3-dependent gene activation in a gene-specific manner through at least two mechanisms; requires only either the sequestering of a general partner such as TCF3 through heterodimerization, either also requires binding of the bHLH domain to DNA via a basic motif (By similarity).
Subunit Structure: Efficient DNA binding requires dimerization with another bHLH protein. Interacts with NEUROG3 AND NEUROD1. Interacts with ZFPM2; mediates indirect interaction with GATA4. Forms a heterodimer with TCF3; repress transcription of TCF3 and TCF3/NEUROG3 dimer-induced transactivation of E box-dependent promoters.
Similarity: The bHLH domain mediates transcriptional repression by inhibiting TCF3 transcriptional activity through heterodimerization.

NCBI and Uniprot Product Information

• NCBI GI #: 226498192
• NCBI GeneID: 84913
• NCBI Accession #: NP_116216.2
• NCBI GenBank Nucleotide #: NM_032827.6
• UniProt Accession #: Q96SQ7; Q504S2; Q659B0
• Molecular Weight: Observed: 35 kDa; Predicted: 35 kDa
• NCBI Official Full Name: protein atonal homolog 8
• NCBI Official Synonym Full Names: atonal bHLH transcription factor 8
• NCBI Official Symbol: ATOH8
• NCBI Official Synonym Symbols: HATH6; bHLHa21
• NCBI Protein Information: protein atonal homolog 8
• UniProt Protein Name: Protein atonal homolog 8
• Protein Family: Protein atonal
• UniProt Gene Name: ATOH8
• UniProt Synonym Gene Names: ATH6; BHLHA21; hATH6

Uniprot Description

Transcription factor that binds a palindromic (canonical) core consensus DNA sequence 5'-CANNTG- 3' known as an E-box element, possibly as a heterodimer with other bHLH proteins (PubMed:24236640). Regulates endothelial cell proliferation, migration and tube-like structures formation (PubMed:24463812). Modulates endothelial cell differentiation through NOS3 (PubMed:24463812). May be implicated in specification and differentiation of neuronal cell lineages in the brain (). May participate in kidney development and may be involved in podocyte differentiation (). During early embryonic development is involved in tissue-specific differentiation processes that are dependent on class II bHLH factors and namely modulates the differentiation program initiated by the pro-endocrine factor NEUROG3 (). During myogenesis, may play a role during the transition of myoblasts from the proliferative phase to the differentiation phase (). Positively regulates HAMP transcription in two ways, firstly by acting directly on the HAMP promoter via E-boxes binding and indirectly through increased phosphorylation of SMAD protein complex (PubMed:24236640). Repress NEUROG3-dependent gene activation in a gene-specific manner through at least two mechanisms; requires only either the sequestering of a general partner such as TCF3 through heterodimerization, either also requires binding of the bHLH domain to DNA via a basic motif ().