Western Blot (WB)
(Figure 1: Western blot analysis using HPTR mouse mAb against truncated HPRT recombinant protein.)
Mouse anti-Human HPRT Monoclonal Antibody | anti-HPRT antibody
HPRT Antibody
Gene Names
HPRT1; HPRT; HGPRT
Reactivity
Human
Applications
Western Blot, ELISA
Purity
Affinity-Chromatography
Synonyms
HPRT; Monoclonal Antibody; HPRT Antibody; HGPRT; HGPRTase; HPRT 1; HPRT_HUMAN; HPRT1; Hypoxanthine guanine phosphoribosyltransferase; Hypoxanthine phosphoribosyltransferase 1 (Lesch Nyhan syndrome); Hypoxanthine phosphoribosyltransferase 1; Hypoxanthine-guanine phosphoribosyltransferase; anti-HPRT antibody
Host
Mouse
Reactivity
Human
Clonality
Monoclonal
Isotype
IgG1
Specificity
HPRT antibody detects endogenous levels of total HPRT
Purity/Purification
Affinity-Chromatography
Form/Format
Phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Concentration
1mg/ml (varies by lot)
Sequence Length
218
Applicable Applications for anti-HPRT antibody
Western Blot (WB), ELISA (EIA)
Application Notes
ELISA: 1:10000
WB: 1:500-1:2000
WB: 1:500-1:2000
Immunogen
Purified recombinant fragment of human HPRT expressed in E. Coli
Subcellular Location
Cytoplasm.
Preparation and Storage
Store at -20 degree C. Stable for 12 months from date of receipt.
Western Blot (WB)
(Figure 1: Western blot analysis using HPTR mouse mAb against truncated HPRT recombinant protein.)
Western Blot (WB)
(Figure 1: Western blot analysis using HPTR mouse mAb against truncated HPRT recombinant protein.)
Related Product Information for anti-HPRT antibody
Description: The HPRT1 gene provides instructions for making an enzyme called hypoxanthine phosphoribosyltransferase 1. This enzyme allows cells to recycle purines, some of the building blocks of DNA and its chemical cousin RNA.The enzyme hypoxanthine-guanine phosphoribosyltrasferase (E.C.2.4.2.8., HPRT) plays a crucial role in uric acid synthesis and purine metabolism.This enzyme catalyzes the conversion of hypoxanthine and guanine to inosine monophosphate (IMP) and guanosine monophosphate (GMP), respectively, and uses phosphoribosylpyrophosphate (PRPP) as a cosubstrate and as a source of energy. This pathway is also known as the purine salvage pathway because it allows cells to reuse purine compounds to build DNA and RNA.
Function: Converts guanine to guanosine monophosphate, and hypoxanthine to inosine monophosphate. Transfers the 5-phosphoribosyl group from 5-phosphoribosylpyrophosphate onto the purine. Plays a central role in the generation of purine nucleotides through the purine salvage pathway.
Subunit Structure: Homotetramer.
Similarity: Belongs to the purine/pyrimidine phosphoribosyltransferase family.
Function: Converts guanine to guanosine monophosphate, and hypoxanthine to inosine monophosphate. Transfers the 5-phosphoribosyl group from 5-phosphoribosylpyrophosphate onto the purine. Plays a central role in the generation of purine nucleotides through the purine salvage pathway.
Subunit Structure: Homotetramer.
Similarity: Belongs to the purine/pyrimidine phosphoribosyltransferase family.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
Observed: 25 kDa
Predicted: 25 kDa
Predicted: 25 kDa
NCBI Official Full Name
hypoxanthine-guanine phosphoribosyltransferase
NCBI Official Synonym Full Names
hypoxanthine phosphoribosyltransferase 1
NCBI Official Symbol
HPRT1
NCBI Official Synonym Symbols
HPRT; HGPRT
NCBI Protein Information
hypoxanthine-guanine phosphoribosyltransferase
UniProt Protein Name
Hypoxanthine-guanine phosphoribosyltransferase
UniProt Gene Name
HPRT1
UniProt Synonym Gene Names
HPRT; HGPRT; HGPRTase
NCBI Description
The protein encoded by this gene is a transferase, which catalyzes conversion of hypoxanthine to inosine monophosphate and guanine to guanosine monophosphate via transfer of the 5-phosphoribosyl group from 5-phosphoribosyl 1-pyrophosphate. This enzyme plays a central role in the generation of purine nucleotides through the purine salvage pathway. Mutations in this gene result in Lesch-Nyhan syndrome or gout.[provided by RefSeq, Jun 2009]
Uniprot Description
Converts guanine to guanosine monophosphate, and hypoxanthine to inosine monophosphate. Transfers the 5-phosphoribosyl group from 5-phosphoribosylpyrophosphate onto the purine. Plays a central role in the generation of purine nucleotides through the purine salvage pathway.
Research Articles on HPRT
Similar Products
Product Notes
The HPRT hprt1 (Catalog #AAA9602462) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. The HPRT Antibody reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's HPRT can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), ELISA (EIA). ELISA: 1:10000 WB: 1:500-1:2000. Researchers should empirically determine the suitability of the HPRT hprt1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "HPRT, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
If you are ready to order, navigate to Shopping Cart and get ready to checkout.
