Western Blot (WB)
(Western blot analysis of HPRT on different lysates using anti-HPRT antibody at 1/500 dilution. Positive control: Lane 1: Hela Lane 2: A431 Lane 3: MCF-7 Lane 4: 293 Lane 5: Rat kidney Lane 6: Rat brain Lane 7: Mouse testis Lane 8: Mouse colon)
Rabbit HPRT Monoclonal Antibody | anti-HPRT antibody
HPRT Antibody
IHC: 1:50-1:200
IP: 1:10-1:50
Western Blot (WB)
(Western blot analysis of HPRT on different lysates using anti-HPRT antibody at 1/500 dilution. Positive control: Lane 1: Hela Lane 2: A431 Lane 3: MCF-7 Lane 4: 293 Lane 5: Rat kidney Lane 6: Rat brain Lane 7: Mouse testis Lane 8: Mouse colon)
Western Blot (WB)
(Western blot analysis of HPRT on different lysates using anti-HPRT antibody at 1/500 dilution. Positive control: Lane 1: Hela Lane 2: A431 Lane 3: MCF-7 Lane 4: 293 Lane 5: Rat kidney Lane 6: Rat brain Lane 7: Mouse testis Lane 8: Mouse colon)
Western Blot (WB)
(Western blot analysis of HPRT on Zebrafish tissue lysates using anti-HPRT antibody at 1/500 dilution.)
Western Blot (WB)
(Western blot analysis of HPRT on Zebrafish tissue lysates using anti-HPRT antibody at 1/500 dilution.)
Immunohistochemistry (IHC)
(Immunohistochemical analysis of paraffin-embedded rat brain tissue using anti-HPRT antibody. Counter stained with hematoxylin.)
Immunohistochemistry (IHC)
(Immunohistochemical analysis of paraffin-embedded rat brain tissue using anti-HPRT antibody. Counter stained with hematoxylin.)
Immunohistochemistry (IHC)
(Immunohistochemical analysis of paraffin-embedded human tonsil tissue using anti-HPRT antibody. Counter stained with hematoxylin.)
Immunohistochemistry (IHC)
(Immunohistochemical analysis of paraffin-embedded human tonsil tissue using anti-HPRT antibody. Counter stained with hematoxylin.)
Immunohistochemistry (IHC)
(Immunohistochemical analysis of paraffin-embedded human colon cancer tissue using anti-HPRT antibody. Counter stained with hematoxylin.)
Immunohistochemistry (IHC)
(Immunohistochemical analysis of paraffin-embedded human colon cancer tissue using anti-HPRT antibody. Counter stained with hematoxylin.)
Immunohistochemistry (IHC)
(Immunohistochemical analysis of paraffin-embedded human colon cancer tissue using anti-HPRT antibody. Counter stained with hematoxylin.)
Immunohistochemistry (IHC)
(Immunohistochemical analysis of paraffin-embedded human colon cancer tissue using anti-HPRT antibody. Counter stained with hematoxylin.)
NCBI and Uniprot Product Information
NCBI Description
The protein encoded by this gene is a transferase, which catalyzes conversion of hypoxanthine to inosine monophosphate and guanine to guanosine monophosphate via transfer of the 5-phosphoribosyl group from 5-phosphoribosyl 1-pyrophosphate. This enzyme plays a central role in the generation of purine nucleotides through the purine salvage pathway. Mutations in this gene result in Lesch-Nyhan syndrome or gout.[provided by RefSeq, Jun 2009]
Uniprot Description
HPRT1: Converts guanine to guanosine monophosphate, and hypoxanthine to inosine monophosphate. Transfers the 5- phosphoribosyl group from 5-phosphoribosylpyrophosphate onto the purine. Plays a central role in the generation of purine nucleotides through the purine salvage pathway. Defects in HPRT1 are the cause of Lesch-Nyhan syndrome (LNS). LNS is characterized by complete lack of enzymatic activity that results in hyperuricemia, choreoathetosis, mental retardation, and compulsive self-mutilation. Defects in HPRT1 are the cause of gout HPRT-related (GOUT-HPRT); also known as HPRT-related gout or Kelley-Seegmiller syndrome. Gout is characterized by partial enzyme activity and hyperuricemia. Belongs to the purine/pyrimidine phosphoribosyltransferase family.
Protein type: Xenobiotic Metabolism - drug metabolism - other enzymes; Nucleotide Metabolism - purine; EC 2.4.2.8; Transferase; Cell development/differentiation
Chromosomal Location of Human Ortholog: Xq26.1
Cellular Component: cytoplasm; cytosol
Molecular Function: protein binding; protein homodimerization activity; magnesium ion binding; nucleotide binding; hypoxanthine phosphoribosyltransferase activity
Biological Process: hypoxanthine metabolic process; IMP salvage; cytolysis; adenine salvage; locomotory behavior; dopamine metabolic process; purine salvage; protein homotetramerization; cerebral cortex neuron differentiation; IMP metabolic process; hypoxanthine salvage; central nervous system neuron development; grooming behavior; lymphocyte proliferation; striatum development; nucleobase, nucleoside and nucleotide metabolic process; GMP salvage; dendrite morphogenesis; response to amphetamine; purine nucleotide biosynthetic process; purine base metabolic process; GMP catabolic process; positive regulation of dopamine metabolic process; purine ribonucleoside salvage; guanine salvage
Disease: Kelley-seegmiller Syndrome; Lesch-nyhan Syndrome
Research Articles on HPRT
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Product Notes
The HPRT hprt1 (Catalog #AAA9436437) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The HPRT Antibody reacts with Human, Mouse, Rat, Zebrafish and may cross-react with other species as described in the data sheet. AAA Biotech's HPRT can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunohistochemistry (IHC), Immunoprecipitation (IP). WB: 1:500-1:2000 IHC: 1:50-1:200 IP: 1:10-1:50. Researchers should empirically determine the suitability of the HPRT hprt1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "HPRT, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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