Immunohistochemistry (IHC)
(MBS9600741 at 1/100 staining Mouse liver tissue by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the antibody for 1.5 hours at 22 degree C. An HRP conjugated goat anti-rabbit antibody was used as the secondary.)
Rabbit anti-Human, Mouse SSH3 Polyclonal Antibody | anti-SSH3 antibody
Phospho-SSH3 (Ser37) Antibody
Gene Names
SSH3; SSH3L
Reactivity
Human, Mouse
Applications
Western Blot, Immunohistochemistry
Purity
From purified rabbit serum by affinity purification via sequential chromatography on phospho-and non-phospho-peptide affinity columns.
Synonyms
SSH3; Polyclonal Antibody; Phospho-SSH3 (Ser37) Antibody; FLJ10928; FLJ20515; hSSH 3L; hSSH-3L; hSSH3L; Protein phosphatase Slingshot homolog 3; Slingshot 3; Slingshot homolog 3; Slingshot3; SSH 3; SSH 3L; SSH-3L; SSH-like protein 3; Ssh3; SSH3_HUMAN; SSH3L; anti-SSH3 antibody
Host
Rabbit
Reactivity
Human, Mouse
Clonality
Polyclonal
Isotype
IgG
Specificity
Phospho-SSH3 (Ser37) antibody detects endogenous levels of SSH3.
Purity/Purification
From purified rabbit serum by affinity purification via sequential chromatography on phospho-and non-phospho-peptide affinity columns.
Form/Format
Liquid
Phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Concentration
1mg/ml (varies by lot)
Sequence Length
659
Applicable Applications for anti-SSH3 antibody
Western Blot (WB), Immunohistochemistry (IHC)
Application Notes
WB: 1:500-1:2000
IHC: 1:50-1:200
IHC: 1:50-1:200
Immunogen
A synthesized peptide derived from human SSH3 around the phosphorylation site of Ser37.
Subcellular Location
Cytoplasm > Cytoskeleton. Nucleus.
Predicted Cross Reactivity
Pig, Bovine, Horse, Sheep, Dog
Similarity
Pig (100%), Bovine (100%), Horse (100%), Sheep (100%), Dog (100%)
Conjugation
Unconjugated
Epitope
Phospho Ser37
Preparation and Storage
Store at -20 degree C. Stable for 12 months from date of receipt.
Immunohistochemistry (IHC)
(MBS9600741 at 1/100 staining Mouse liver tissue by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the antibody for 1.5 hours at 22 degree C. An HRP conjugated goat anti-rabbit antibody was used as the secondary.)
Immunohistochemistry (IHC)
(MBS9600741 at 1/100 staining Mouse liver tissue by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the antibody for 1.5 hours at 22 degree C. An HRP conjugated goat anti-rabbit antibody was used as the secondary.)
Western Blot (WB)
(Western blot analysis of extracts of ZR-75-1 cells, using Phospho-SSH3 (Ser37) Antibody.)
Western Blot (WB)
(Western blot analysis of extracts of ZR-75-1 cells, using Phospho-SSH3 (Ser37) Antibody.)
Related Product Information for anti-SSH3 antibody
Function: Protein phosphatase which may play a role in the regulation of actin filament dynamics. Can dephosphorylate and activate the actin binding/depolymerizing factor cofilin, which subsequently binds to actin filaments and stimulates their disassembly (By similarity).
Subunit Structure: Does not bind to, or colocalize with, filamentous actin.
Similarity: Belongs to the protein-tyrosine phosphatase family.
Subunit Structure: Does not bind to, or colocalize with, filamentous actin.
Similarity: Belongs to the protein-tyrosine phosphatase family.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
Observed: 90 kDa
Predicted: 73 kDa
Predicted: 73 kDa
NCBI Official Full Name
protein phosphatase Slingshot homolog 3
NCBI Official Synonym Full Names
slingshot protein phosphatase 3
NCBI Official Symbol
SSH3
NCBI Official Synonym Symbols
SSH3L
NCBI Protein Information
protein phosphatase Slingshot homolog 3
UniProt Protein Name
Protein phosphatase Slingshot homolog 3
Protein Family
UniProt Gene Name
SSH3
UniProt Synonym Gene Names
SSH3L; SSH-3L; hSSH-3L
NCBI Description
The ADF (actin-depolymerizing factor)/cofilin family (see MIM 601442) is composed of stimulus-responsive mediators of actin dynamics. ADF/cofilin proteins are inactivated by kinases such as LIM domain kinase-1 (LIMK1; MIM 601329). The SSH family appears to play a role in actin dynamics by reactivating ADF/cofilin proteins in vivo (Niwa et al., 2002 [PubMed 11832213]).[supplied by OMIM, Mar 2008]
Uniprot Description
Protein phosphatase which may play a role in the regulation of actin filament dynamics. Can dephosphorylate and activate the actin binding/depolymerizing factor cofilin, which subsequently binds to actin filaments and stimulates their disassembly ().
Research Articles on SSH3
Similar Products
Product Notes
The SSH3 ssh3 (Catalog #AAA9600741) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Phospho-SSH3 (Ser37) Antibody reacts with Human, Mouse and may cross-react with other species as described in the data sheet. AAA Biotech's SSH3 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunohistochemistry (IHC). WB: 1:500-1:2000 IHC: 1:50-1:200. Researchers should empirically determine the suitability of the SSH3 ssh3 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "SSH3, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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