Western Blot (WB)
(Western blot analysis of PKCB1 expression in Mouse brain lysate)
Rabbit anti-Human, Mouse PKCB1 Polyclonal Antibody | anti-PKCB1 antibody
PKCB1 Antibody
Gene Names
ZMYND8; RACK7; PRKCBP1; PRO2893
Reactivity
Human, Mouse
Applications
Western Blot, Immunofluorescence, Immunocytochemistry, ELISA
Purity
The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin.
Synonyms
PKCB1; Polyclonal Antibody; PKCB1 Antibody; CTCL tumor antigen se14 3; Cutaneous T cell lymphoma associated antigen se14 3; KIAA1125; MGC31836; predicted protein of HQ2893; PRKCBP 1; PRO2893; Protein kinase C binding protein 1; Rack7; Zinc finger MYND domain containing protein 8; ZMYND 8; ZMYND8; anti-PKCB1 antibody
Host
Rabbit
Reactivity
Human, Mouse
Clonality
Polyclonal
Isotype
IgG
Specificity
PKCB1 antibody detects endogenous levels of PKCB1
Purity/Purification
The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin.
Form/Format
Liquid
Phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Concentration
1mg/ml (varies by lot)
Sequence Length
1054
Applicable Applications for anti-PKCB1 antibody
Western Blot (WB), Immunofluorescence (IF), Immunocytochemistry (ICC), ELISA (EIA)
Application Notes
WB: 1:500-1:2000
IF/ICC: 1:100-1:500
IF/ICC: 1:100-1:500
Immunogen
A synthesized peptide derived from human PKCB1
Subcellular Location
Nuclear
Tissue Specificity
Expressed in all tissues examined with highest expression in brain, lung, pancreas, and placenta. Expressed in cutaneous T-cell lymphomas (CTCL).
Predicted Cross Reactivity
Pig, Bovine, Dog
Similarity
Pig (91%), Bovine (82%), Dog (91%)
Conjugation
Unconjugated
Preparation and Storage
Store at -20 degree C. Stable for 12 months from date of receipt.
Western Blot (WB)
(Western blot analysis of PKCB1 expression in Mouse brain lysate)
Western Blot (WB)
(Western blot analysis of PKCB1 expression in Mouse brain lysate)
Western Blot (WB)
(Western blot analysis on 293 cell lysate using PKCB1 Antibody, The lane on the left is treated with the antigen-specific peptide. )
Western Blot (WB)
(Western blot analysis on 293 cell lysate using PKCB1 Antibody, The lane on the left is treated with the antigen-specific peptide.
)
Immunofluorescence (IF)
(MBS9600380 staining 293 by IF/ICC. The sample were fixed with PFA and permeabilized in 0.1% Triton X-100, then blocked in 10% serum for 45 minutes at 25 degree C. The primary antibody was diluted at 1/200 and incubated with the sample for 1 hour at 37 degree C. An Alexa Fluor 594 conjugated goat anti-rabbit IgG (H+L) Ab, diluted at 1/600, was used as the secondary antibody.)
Immunofluorescence (IF)
(MBS9600380 staining 293 by IF/ICC. The sample were fixed with PFA and permeabilized in 0.1% Triton X-100, then blocked in 10% serum for 45 minutes at 25 degree C. The primary antibody was diluted at 1/200 and incubated with the sample for 1 hour at 37 degree C. An Alexa Fluor 594 conjugated goat anti-rabbit IgG (H+L) Ab, diluted at 1/600, was used as the secondary antibody.)
Related Product Information for anti-PKCB1 antibody
Description: The protein encoded by this gene is a receptor for activated C-kinase (RACK) protein. The encoded protein has been shown to bind in vitro to activated protein kinase C beta I. In addition, this protein is a cutaneous T-cell lymphoma-associated antigen. Finally, the protein contains a bromodomain and two zinc fingers, and is thought to be a transcriptional regulator. Multiple transcript variants encoding several different isoforms have been found for this gene.
Function: May act as a transcriptional corepressor for KDM5D. Required for KDM5D-mediated down-regulation of diverse metastasis-associated genes; the function seems to involve the recognition of the dual histone signature H3K4me1-H3K14ac. Suppresses prostate cancer cell invasion.
Subunit Structure: Interacts in vitro with PRKCB1 (PubMed:11003709). Interacts with KDM5D and KDM1A. Interacts with histone H3 (via N-terminus) that is both methylated at 'Lys-4' (H3K4me1) and acetylated at 'Lys-14' (H3K14ac), and with histone H3 (via N-terminus) unmodified at 'Lys-4' (H3K4me0) and acetylated at 'Lys-14' (H3K14ac).
Function: May act as a transcriptional corepressor for KDM5D. Required for KDM5D-mediated down-regulation of diverse metastasis-associated genes; the function seems to involve the recognition of the dual histone signature H3K4me1-H3K14ac. Suppresses prostate cancer cell invasion.
Subunit Structure: Interacts in vitro with PRKCB1 (PubMed:11003709). Interacts with KDM5D and KDM1A. Interacts with histone H3 (via N-terminus) that is both methylated at 'Lys-4' (H3K4me1) and acetylated at 'Lys-14' (H3K14ac), and with histone H3 (via N-terminus) unmodified at 'Lys-4' (H3K4me0) and acetylated at 'Lys-14' (H3K14ac).
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
Molecular Weight
Observed: 132 kDa
Predicted: 132 kDa
Predicted: 132 kDa
NCBI Official Full Name
protein kinase C-binding protein 1 isoform e
NCBI Official Synonym Full Names
zinc finger MYND-type containing 8
NCBI Official Symbol
ZMYND8
NCBI Official Synonym Symbols
RACK7; PRKCBP1; PRO2893
NCBI Protein Information
protein kinase C-binding protein 1
UniProt Protein Name
Protein kinase C-binding protein 1
UniProt Gene Name
ZMYND8
UniProt Synonym Gene Names
KIAA1125; PRKCBP1; RACK7; CTCL-associated antigen se14-3
NCBI Description
The protein encoded by this gene is a receptor for activated C-kinase (RACK) protein. The encoded protein has been shown to bind in vitro to activated protein kinase C beta I. In addition, this protein is a cutaneous T-cell lymphoma-associated antigen. Finally, the protein contains a bromodomain and two zinc fingers, and is thought to be a transcriptional regulator. Multiple transcript variants encoding several different isoforms have been found for this gene. [provided by RefSeq, Jul 2008]
Uniprot Description
May act as a transcriptional corepressor for KDM5D. Required for KDM5D-mediated down-regulation of diverse metastasis-associated genes; the function seems to involve the recognition of the dual histone signature H3K4me1-H3K14ac. Suppresses prostate cancer cell invasion.
Research Articles on PKCB1
Similar Products
Product Notes
The PKCB1 zmynd8 (Catalog #AAA9600380) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The PKCB1 Antibody reacts with Human, Mouse and may cross-react with other species as described in the data sheet. AAA Biotech's PKCB1 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunofluorescence (IF), Immunocytochemistry (ICC), ELISA (EIA). WB: 1:500-1:2000 IF/ICC: 1:100-1:500. Researchers should empirically determine the suitability of the PKCB1 zmynd8 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "PKCB1, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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