Rabbit anti-Human, Mouse ZFP598 Polyclonal Antibody | anti-ZFP598 antibody

ZFP598 (Phospho-Tyr306) Antibody

Cat. Num. AAA9508559

• Gene Names: ZNF598; HEL2
• Reactivity: Human, Mouse
• Applications: Western Blot, ELISA
• Purity: Purified from rabbit antiserum by affinity-chromatography using phospho peptide. The antibody against non-phospho peptide was removed by chromatography using corresponding non-phospho peptide.
• Synonyms: ZFP598; Polyclonal Antibody; ZFP598 (Phospho-Tyr306) Antibody; anti-ZFP598 antibody

• Host: Rabbit
• Reactivity: Human, Mouse
• Clonality: Polyclonal
• Specificity: ZFP598 (Phospho-Tyr306) antibody detects endogenous levels of ZFP598 only when phosphorylated at Tyr306.
• Purity/Purification: Purified from rabbit antiserum by affinity-chromatography using phospho peptide. The antibody against non-phospho peptide was removed by chromatography using corresponding non-phospho peptide.
• Form/Format: Rabbit IgG in phosphate buffered saline (Phospho-without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
• Concentration: 1 mg/ml (varies by lot)
• Sequence Length: 341

• Applicable Applications for anti-ZFP598 antibody: Western Blot (WB), ELISA (EIA)
• Application Notes: WB: 1:500~1:1000; ELISA: 1:10000
• Target Modification: Phospho
• Modification Sites: H:Y306; M:Y304
• Immunogen: The antiserum was produced against synthesized phospho-peptide derived from human ZFP598 around the phosphorylation site of Tyr306.
• Preparation and Storage: Stable at -20 degree C for at least 1 year.

ELISA (EIA)

(Enzyme-Linked Immunosorbent Assay (Phospho-ELISA) for Immunogen Phosphopeptide (Phospho-left) and Non-Phosphopeptide (Phospho-right), using ZFP598 (Phospho-Tyr306) Antibody)

Western Blot (WB)

(Western blot analysis of lysate from Jurkat cells, uisng phospho-ZFP598 (Phospho-Tyr306) antibody.)

NCBI and Uniprot Product Information

• NCBI GI #: 409264581
• NCBI GeneID: 90850
• NCBI Accession #: NP_835461.2
• NCBI GenBank Nucleotide #: NM_178167.3
• UniProt Accession #: Q86UK7; Q8IW49; Q8N3D9; Q96FG3; Q9H7J3
• Molecular Weight: 98 kDa
• NCBI Official Full Name: E3 ubiquitin-protein ligase ZNF598
• NCBI Official Synonym Full Names: zinc finger protein 598
• NCBI Official Symbol: ZNF598
• NCBI Official Synonym Symbols: HEL2
• NCBI Protein Information: E3 ubiquitin-protein ligase ZNF598
• UniProt Protein Name: E3 ubiquitin-protein ligase ZNF598
• UniProt Gene Name: ZNF598

NCBI Description

Zinc-finger proteins bind nucleic acids and play important roles in various cellular functions, including cell proliferation, differentiation, and apoptosis. This protein and Grb10-interacting GYF protein 2 have been identified as a components of the mammalian 4EHP (m4EHP) complex. The complex is thought to function as a translation repressor in embryonic development. [provided by RefSeq, Oct 2012]

Uniprot Description

E3 ubiquitin-protein ligase that plays a key role in the ribosome quality control (RQC), a pathway that takes place when a ribosome has stalled during translation (PubMed:28065601, PubMed:28132843). Required for ribosomes to terminally stall during translation of poly(A) sequences by mediating monoubiquitination of 40S ribosomal protein RPS10/eS10, RPS20/uS10 and RPS3/uS3 (PubMed:28065601, PubMed:28132843). Stalling precludes synthesis of a long poly-lysine tail and initiates the RQC pathway to degrade the potentially detrimental aberrant nascent polypeptide (PubMed:28065601, PubMed:28132843). Also acts as a component of the 4EHP-GYF2 complex, a multiprotein complex that acts as a repressor of translation initiation (PubMed:22751931).