Rabbit anti-Human PDLIM5 Polyclonal Antibody | anti-PDLIM5 antibody

PDLIM5 (Phospho-Tyr251) Antibody

Cat. Num. AAA9508533

• Gene Names: PDLIM5; L9; ENH; LIM; ENH1
• Reactivity: Human
• Applications: Western Blot, ELISA
• Purity: Purified from rabbit antiserum by affinity-chromatography using phospho peptide. The antibody against non-phospho peptide was removed by chromatography using corresponding non-phospho peptide.
• Synonyms: PDLIM5; Polyclonal Antibody; PDLIM5 (Phospho-Tyr251) Antibody; anti-PDLIM5 antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Specificity: PDLIM5 (Phospho-Tyr251) antibody detects endogenous levels of PDLIM5 only when phosphorylated at Tyr251.
• Purity/Purification: Purified from rabbit antiserum by affinity-chromatography using phospho peptide. The antibody against non-phospho peptide was removed by chromatography using corresponding non-phospho peptide.
• Form/Format: Rabbit IgG in phosphate buffered saline (Phospho-without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
• Concentration: 1 mg/ml (varies by lot)
• Sequence Length: 483

• Applicable Applications for anti-PDLIM5 antibody: Western Blot (WB), ELISA (EIA)
• Application Notes: WB: 1:500~1:1000; ELISA: 1:10000
• Target Modification: Phospho
• Modification Sites: H:Y251
• Immunogen: The antiserum was produced against synthesized phospho-peptide derived from human PDLIM5 around the phosphorylation site of Tyr251.
• Preparation and Storage: Stable at -20 degree C for at least 1 year.

ELISA (EIA)

(Enzyme-Linked Immunosorbent Assay (Phospho-ELISA) for Immunogen Phosphopeptide (Phospho-left) and Non-Phosphopeptide (Phospho-right), using PDLIM5 (Phospho-Tyr251) Antibody)

Western Blot (WB)

(Western blot analysis of lysate from K562 cells, uisng phospho-PDLIM5 (Phospho-Tyr251) antibody.)

NCBI and Uniprot Product Information

• NCBI GI #: 58533159
• NCBI GeneID: 10611
• NCBI Accession #: NP_001011515.1
• NCBI GenBank Nucleotide #: NM_001011515.2
• UniProt Accession #: Q96HC4; O60705; Q56VN4; Q5UW38; Q8WVK0; A8K6F9; D6RB78; E9PBF5
• Molecular Weight: 64 kDa
• NCBI Official Full Name: PDZ and LIM domain protein 5 isoform d
• NCBI Official Synonym Full Names: PDZ and LIM domain 5
• NCBI Official Symbol: PDLIM5
• NCBI Official Synonym Symbols: L9; ENH; LIM; ENH1
• NCBI Protein Information: PDZ and LIM domain protein 5
• UniProt Protein Name: PDZ and LIM domain protein 5
• Protein Family: PDZ and LIM domain protein
• UniProt Gene Name: PDLIM5
• UniProt Synonym Gene Names: ENH

NCBI Description

This gene encodes a member of a family of proteins that possess a 100-amino acid PDZ domain at the N terminus and one to three LIM domains at the C-terminus. This family member functions as a scaffold protein that tethers protein kinases to the Z-disk in striated muscles. It is thought to function in cardiomyocyte expansion and in restraining postsynaptic growth of excitatory synapses. Alternative splicing of this gene results in multiple transcript variants. [provided by RefSeq, Jan 2012]

Uniprot Description

May play an important role in the heart development by scaffolding PKC to the Z-disk region. May play a role in the regulation of cardiomyocyte expansion. Overexpression promotes the development of heart hypertrophy. Contributes to the regulation of dendritic spine morphogenesis in neurons. May restrain postsynaptic growth of excitatory synapses ().