Immunofluorescence (IF)
(Immunofluorescence analysis of COS7 cells, using DAPK1 Antibody. The picture on the right is blocked with the synthesized peptide.)
Rabbit anti-Human, Mouse DAPK1 Polyclonal Antibody | anti-DAPK1 antibody
DAPK1 Antibody
IF: 1:100~1:500
ELISA: 1:1000
Immunofluorescence (IF)
(Immunofluorescence analysis of COS7 cells, using DAPK1 Antibody. The picture on the right is blocked with the synthesized peptide.)
Immunofluorescence (IF)
(Immunofluorescence analysis of COS7 cells, using DAPK1 Antibody. The picture on the right is blocked with the synthesized peptide.)
Immunohistochemistry (IHC)
(Immunohistochemistry analysis of paraffin-embedded human liver carcinoma tissue, using DAPK1 Antibody. The picture on the right is blocked with the synthesized peptide.)
Immunohistochemistry (IHC)
(Immunohistochemistry analysis of paraffin-embedded human liver carcinoma tissue, using DAPK1 Antibody. The picture on the right is blocked with the synthesized peptide.)
NCBI and Uniprot Product Information
NCBI Description
Death-associated protein kinase 1 is a positive mediator of gamma-interferon induced programmed cell death. DAPK1 encodes a structurally unique 160-kD calmodulin dependent serine-threonine kinase that carries 8 ankyrin repeats and 2 putative P-loop consensus sites. It is a tumor suppressor candidate. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Dec 2013]
Uniprot Description
Calcium/calmodulin-dependent serine/threonine kinase involved in multiple cellular signaling pathways that trigger cell survival, apoptosis, and autophagy. Regulates both type I apoptotic and type II autophagic cell deaths signal, depending on the cellular setting. The former is caspase-dependent, while the latter is caspase-independent and is characterized by the accumulation of autophagic vesicles. Phosphorylates PIN1 resulting in inhibition of its catalytic activity, nuclear localization, and cellular function. Phosphorylates TPM1, enhancing stress fiber formation in endothelial cells. Phosphorylates STX1A and significantly decreases its binding to STXBP1. Phosphorylates PRKD1 and regulates JNK signaling by binding and activating PRKD1 under oxidative stress. Phosphorylates BECN1, reducing its interaction with BCL2 and BCL2L1 and promoting the induction of autophagy. Phosphorylates TSC2, disrupting the TSC1-TSC2 complex and stimulating mTORC1 activity in a growth factor-dependent pathway. Phosphorylates RPS6, MYL9 and DAPK3. Acts as a signaling amplifier of NMDA receptors at extrasynaptic sites for mediating brain damage in stroke. Cerebral ischemia recruits DAPK1 into the NMDA receptor complex and it phosphorylates GRINB at Ser-1303 inducing injurious Ca2+ influx through NMDA receptor channels, resulting in an irreversible neuronal death. Required together with DAPK3 for phosphorylation of RPL13A upon interferon-gamma activation which is causing RPL13A involvement in transcript-selective translation inhibition.
Research Articles on DAPK1
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Product Notes
The DAPK1 dapk1 (Catalog #AAA9503845) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The DAPK1 Antibody reacts with Human, Mouse and may cross-react with other species as described in the data sheet. AAA Biotech's DAPK1 can be used in a range of immunoassay formats including, but not limited to, Immunohistochemistry (IHC), Immunofluorescence (IF), ELISA (EIA). IHC: 1:50~1:100 IF: 1:100~1:500 ELISA: 1:1000. Researchers should empirically determine the suitability of the DAPK1 dapk1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "DAPK1, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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