ATPase elisa kit

ATPase Colorimetric Cell-Based ELISA Kit

Cat. Num. AAA9500961

• Reactivity: Human, Mouse, Rat
• Synonyms: ATPase; ATPase Colorimetric Cell-Based ELISA Kit; A1A1; AT1A1; Na+/K+ transporting; alpha 1 polypeptide; Hypothetical protein MGC38419; Na+/K+ ATPase 1; Na; K-ATPase 1; Sodium pump 1; Sodium/potassium-transporting ATPase alpha-1 chain precursor; ATPase elisa kit

• Reactivity: Human, Mouse, Rat
• Form/Format: One 96-Well Plate
• Sequence Length: 1023

• Samples: Researchers plate their cell line of choice
• Assay Type: Non-Phospho Cell-Based ELISA
• Sensitivity: >=5000 cells/well
• Detection Method: Colorimetric 450 nm
• Preparation and Storage: Store at 4 degree C.

Product Categories/Family for ATPase elisa kit

Cell Based ELISA Kit

NCBI and Uniprot Product Information

• NCBI GI #: 21361181
• NCBI GeneID: 476
• NCBI Accession #: NP_000692.2
• NCBI GenBank Nucleotide #: NM_000701.7
• UniProt Accession #: P05023; Q16689; Q6LDM4; Q9UCN1; Q9UJ20; Q9UJ21; B2RBR6; B7Z2T5; B7Z3U6; F5H3A1
• Molecular Weight: 112 kDa
• NCBI Official Full Name: sodium/potassium-transporting ATPase subunit alpha-1 isoform a
• NCBI Official Synonym Full Names: ATPase Na+/K+ transporting subunit alpha 1
• NCBI Official Symbol: ATP1A1
• NCBI Protein Information: sodium/potassium-transporting ATPase subunit alpha-1
• UniProt Protein Name: Sodium/potassium-transporting ATPase subunit alpha-1
• Protein Family: ATPase
• UniProt Gene Name: ATP1A1
• UniProt Synonym Gene Names: Na(+)/K(+) ATPase alpha-1 subunit

NCBI Description

The protein encoded by this gene belongs to the family of P-type cation transport ATPases, and to the subfamily of Na+/K+ -ATPases. Na+/K+ -ATPase is an integral membrane protein responsible for establishing and maintaining the electrochemical gradients of Na and K ions across the plasma membrane. These gradients are essential for osmoregulation, for sodium-coupled transport of a variety of organic and inorganic molecules, and for electrical excitability of nerve and muscle. This enzyme is composed of two subunits, a large catalytic subunit (alpha) and a smaller glycoprotein subunit (beta). The catalytic subunit of Na+/K+ -ATPase is encoded by multiple genes. This gene encodes an alpha 1 subunit. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, May 2009]

Uniprot Description

ATP1A1: the catalytic component of the active enzyme, which catalyzes the hydrolysis of ATP coupled with the exchange of Na and K ions across the plasma membrane. This action creates the electrochemical gradient of Na and K, providing the energy for active transport of various nutrients. Two splice-variant isoforms have been described.

Protein type: EC 3.6.3.9; Hydrolase; Membrane protein, integral; Membrane protein, multi-pass; Transporter; Transporter, ion channel

Chromosomal Location of Human Ortholog: 1p13.1

Cellular Component: apical plasma membrane; basolateral plasma membrane; caveola; endoplasmic reticulum; endosome; extracellular exosome; Golgi apparatus; integral component of membrane; intercalated disc; melanosome; membrane; myelin sheath; plasma membrane; postsynaptic density; protein complex; sarcolemma; sodium:potassium-exchanging ATPase complex; T-tubule

Molecular Function: ADP binding; ankyrin binding; ATP binding; chaperone binding; phosphoinositide 3-kinase binding; phosphoric monoester hydrolase activity; potassium ion binding; protein binding; protein domain specific binding; protein kinase binding; sodium ion binding; sodium:potassium-exchanging ATPase activity; steroid hormone binding

Biological Process: cardiac muscle cell action potential involved in contraction; cell communication by electrical coupling involved in cardiac conduction; cellular potassium ion homeostasis; cellular response to steroid hormone stimulus; cellular sodium ion homeostasis; dephosphorylation; establishment or maintenance of transmembrane electrochemical gradient; membrane hyperpolarization; membrane repolarization; membrane repolarization during cardiac muscle cell action potential; negative regulation of glucocorticoid biosynthetic process; negative regulation of heart contraction; positive regulation of heart contraction; positive regulation of striated muscle contraction; potassium ion import; potassium ion import across plasma membrane; regulation of blood pressure; regulation of cardiac muscle cell contraction; regulation of sodium ion transport; regulation of the force of heart contraction; response to drug