BUD31 elisa kit
BUD31 Colorimetric Cell-Based ELISA Kit
Gene Names
BUD31; G10; EDG2; Cwc14; EDG-2; fSAP17; YCR063W
Reactivity
Human, Mouse, Rat
Synonyms
BUD31; BUD31 Colorimetric Cell-Based ELISA Kit; EDG-2; G10; BUD31 elisa kit
Reactivity
Human, Mouse, Rat
Form/Format
One 96-Well Plate
Sequence Length
144
Preparation and Storage
Store at 4 degree C.
Related Product Information for BUD31 elisa kit
Background/Introduction: The Colorimetric Cell-Based ELISA Kit allows for the detection of various target proteins and the effects that certain stimulation conditions have on target protein expression in different cell lines. Qualitative determination of target protein concentration is achieved by an indirect ELISA format. In essence, the target protein is captured by target-specific primary (1 degree) antibodies while the HRP-conjugated secondary (2 degree) antibodies bind the Fc region of the 1 degree antibody. Through this binding, the HRP enzyme conjugated to the 2 degree antibody can catalyze a colorimetric reaction upon substrate addition. Due to the qualitative nature of the Cell-Based ELISA, multiple normalization methods are described: 1) a monoclonal antibody specific for human GAPDH is included to serve as an internal positive control in normalizing the target absorbance values. 2) Following the colorimetric measurement of HRP activity via substrate addition, the Crystal Violet whole-cell staining method is used to determine cell density. After staining, the results can be analyzed by normalizing the absorbance values to cell amounts, by which the plating difference can be adjusted. 3) If a phosphorylated target is being detected, an antibody against the non-phosphorylated counterpart will be provided for normalization purposes. The absorbance values obtained for the non-phosphorylated target can be used to normalize the absorbance values for the phosphorylated target.
Product Categories/Family for BUD31 elisa kit
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
17 kDa
NCBI Official Full Name
protein BUD31 homolog
NCBI Official Synonym Full Names
BUD31 homolog
NCBI Official Symbol
BUD31
NCBI Official Synonym Symbols
G10; EDG2; Cwc14; EDG-2; fSAP17; YCR063W
NCBI Protein Information
protein BUD31 homolog
UniProt Protein Name
Protein BUD31 homolog
Protein Family
UniProt Gene Name
BUD31
UniProt Synonym Gene Names
EDG2
Uniprot Description
BUD31: Belongs to the BUD31 (G10) family.
Protein type: Spliceosome; Transcription factor
Chromosomal Location of Human Ortholog: 7q22.1
Cellular Component: nuclear chromatin; nucleoplasm; nucleus; spliceosomal complex
Molecular Function: DNA binding transcription factor activity; ligand-dependent nuclear receptor transcription coactivator activity; nuclear hormone receptor binding; protein binding
Biological Process: mRNA splicing, via spliceosome; regulation of transcription from RNA polymerase II promoter
Research Articles on BUD31
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Product Notes
The Human, Mouse, Rat BUD31 bud31 (Catalog #AAA9500750) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA9500750 ELISA Kit recognizes Human, Mouse, Rat BUD31. It is sometimes possible for the material contained within the vial of "BUD31, ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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