Human delta-like 3 (Drosophila) ELISA Kit | DLL3 elisa kit

Human Delta-like protein 3, DLL3 ELISA Kit

Cat. Num. AAA947181

• Gene Names: DLL3; SCDO1
• Reactivity: Human
• Synonyms: delta-like 3 (Drosophila); Human Delta-like protein 3; DLL3 ELISA Kit; Human Delta-like protein 3 (DLL3) ELISA kit; SCDO1; delta-like 3 protein; DLL3 elisa kit

• Reactivity: Human
• Specificity: This assay has high sensitivity and excellent specificity for detection of human DLL3. No significant cross-reactivity or interference between human DLL3 and analogues was observed.
• Sequence Length: 618

• Samples: Serum, plasma, tissue homogenates
• Assay Type: Quantitative Sandwich
• Detection Range: 18.75 pg/ml-1200 pg/ml
• Sensitivity: < 4.68 pg/ml
• Intra-assay Precision: Intra-assay Precision (Precision within an assay): CV%<8%. Three samples of known concentration were tested twenty times on one plate to assess.
• Inter-assay Precision: Inter-assay Precision (Precision between assays): CV%<10%. Three samples of known concentration were tested in twenty assays to assess.
• Preparation and Storage: Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for DLL3 elisa kit

Principle of the Assay: This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for DLL3 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any DLL3 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for DLL3 is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of DLL3 bound in the initial step. The color development is stopped and the intensity of the color is measured.

NCBI and Uniprot Product Information

• NCBI GI #: 8393264
• NCBI GeneID: 10683
• NCBI Accession #: NP_058637.1
• NCBI GenBank Nucleotide #: NM_016941.3
• UniProt Accession #: Q9NYJ7; Q8NBS4; E9PFG2
• Molecular Weight: 64,618 Da
• NCBI Official Full Name: delta-like protein 3 isoform 1
• NCBI Official Synonym Full Names: delta-like 3 (Drosophila)
• NCBI Official Symbol: DLL3
• NCBI Official Synonym Symbols: SCDO1
• NCBI Protein Information: delta-like protein 3; delta3; drosophila Delta homolog 3
• UniProt Protein Name: Delta-like protein 3
• Protein Family: Delta-like protein
• UniProt Gene Name: DLL3
• UniProt Synonym Gene Names: Delta3
• UniProt Entry Name: DLL3_HUMAN

NCBI Description

This gene encodes a member of the delta protein ligand family. This family functions as Notch ligands that are characterized by a DSL domain, EGF repeats, and a transmembrane domain. Mutations in this gene cause autosomal recessive spondylocostal dysostosis 1. Two transcript variants encoding distinct isoforms have been identified for this gene. [provided by RefSeq, Jul 2008]

Uniprot Description

DLL3: Inhibits primary neurogenesis. May be required to divert neurons along a specific differentiation pathway. Plays a role in the formation of somite boundaries during segmentation of the paraxial mesoderm. Can bind and activate Notch-1 or another Notch receptor.

Protein type: Membrane protein, integral; Cell development/differentiation

Chromosomal Location of Human Ortholog: 19q13

Cellular Component: integral to membrane

Molecular Function: Notch binding

Biological Process: compartment specification; paraxial mesoderm development; Notch signaling pathway; somitogenesis; skeletal development; negative regulation of neurogenesis

Disease: Spondylocostal Dysostosis 1, Autosomal Recessive