Related Product Information for anti-HDAC9 antibody
In the intact cell, DNA closely associates with histones and other nuclear proteins to form chromatin. The remodeling of chromatin is a critical component of transcriptional regulation and the acetylation of nucleosomal histones is a major source of this remodeling. Acetylation of lysine residues in the amino terminal tail domain of histone results in an allosteric change in the nucleosomal conformation and an increased accessibility to transcription factors by DNA. Several mammalian proteins function as nuclear histone acetylases, including GCN5, PCAF (p300/CBP-associated factor), p300/CBP, HAT1 and the TFIID subunit TAF II p250. Conversely, the deacetylation of histones is associated with transcriptional silencing. The histone deacetylases (HDAC) include HDAC1-9. HDAC9 and HDAC9a are two alternatively spliced isoforms of HDAC9. HDAC9a is 132 amino acids shorter than HDAC9, but both isoforms contain the HDAC catalytic domain, remain capable of deacetylase activity and repress myoctye enhancer-binding factor 2-mediated transcription.
NCBI Description
Histones play a critical role in transcriptional regulation, cell cycle progression, and developmental events. Histone acetylation/deacetylation alters chromosome structure and affects transcription factor access to DNA. The protein encoded by this gene has sequence homology to members of the histone deacetylase family. This gene is orthologous to the Xenopus and mouse MITR genes. The MITR protein lacks the histone deacetylase catalytic domain. It represses MEF2 activity through recruitment of multicomponent corepressor complexes that include CtBP and HDACs. This encoded protein may play a role in hematopoiesis. Multiple alternatively spliced transcripts have been described for this gene but the full-length nature of some of them has not been determined. [provided by RefSeq, Jul 2008]
Uniprot Description
HDAC9: a transcriptional regulator of the histone deacetylase family, subfamily 2. Deacetylates lysine residues on the N-terminal part of the core histones H2A, H2B, H3 AND H4. Plays an important role in transcriptional regulation, cell cycle progression and developmental events. Histone deacetylases act via the formation of large multiprotein complexes.
Protein type: Transcription, coactivator/corepressor; Deacetylase; EC 3.5.1.98
Chromosomal Location of Human Ortholog: 7p21.1
Cellular Component: nucleoplasm; histone methyltransferase complex; transcription factor complex; histone deacetylase complex; cytoplasm; nucleus
Molecular Function: protein binding; NAD-dependent histone deacetylase activity (H3-K9 specific); protein kinase C binding; NAD-dependent histone deacetylase activity (H3-K14 specific); histone deacetylase binding; metal ion binding; protein deacetylase activity; NAD-dependent histone deacetylase activity (H4-K16 specific); histone deacetylase activity; transcription corepressor activity; transcription factor binding
Biological Process: Notch signaling pathway; regulation of skeletal muscle fiber development; B cell activation; transcription, DNA-dependent; cellular response to insulin stimulus; regulation of striated muscle cell differentiation; B cell differentiation; heart development; histone deacetylation; negative regulation of transcription from RNA polymerase II promoter; inflammatory response; negative regulation of transcription, DNA-dependent
Product Notes
The HDAC9 hdac9 (Catalog #
AAA9457309) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The HDAC9 Conjugated Antibody reacts with Human, Rat and may cross-react with other species as described in the data sheet. AAA Biotech's HDAC9 can be used in a range of immunoassay formats including, but not limited to, Most Applications. Suggested Dilution: AF350 conjugated: most applications: 1:50-1:250 AF405 conjugated: most applications: 1:50-1:250 AF488 conjugated: most applications: 1:50-1:250 AF555 conjugated: most applications: 1:50-1:250 AF594 conjugated: most applications: 1:50-1:250 AF647 conjugated: most applications: 1:50-1:250 AF680 conjugated: most applications: 1:50-1:250 AF750 conjugated: most applications: 1:50-1:250 Biotin conjugated: working with enzyme-conjugated streptavidin, most applications: 1:50-1:1000. Researchers should empirically determine the suitability of the HDAC9 hdac9 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process.
It is sometimes possible for the material contained within the vial of
"HDAC9, Monoclonal Antibody" to become dispersed throughout the inside of
the vial, particularly around the seal of said vial, during shipment and storage. We always
suggest centrifuging these vials
to consolidate all of the liquid away from the lid and to the bottom of the vial prior to
opening. Please be advised that
certain products may require dry ice for shipping and that, if this is the case, an
additional dry ice fee may also be
required.
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absolutely not suitable for use in any
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purchaser may be intending to use a product outside of our accepted criteria.
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