Rabbit HDAC9 Polyclonal Antibody | anti-HDAC9 antibody

HDAC9 Polyclonal Antibody

Cat. Num. AAA3007299

• Gene Names: HDAC9; HD7; HD9; HD7b; HDAC; HDRP; MITR; HDAC7; HDAC7B; HDAC9B; HDAC9FL
• Reactivity: Human, Mouse, Rat
• Applications: Western Blot
• Purity: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is >95% (by SDS-PAGE).
• Synonyms: HDAC9; Polyclonal Antibody; HDAC9 Polyclonal Antibody; Histone deacetylase 9 (EC: 3.5.1.98); HD9; Histone deacetylase 7B; HD7; HD7b; Histone deacetylase-related protein; MEF2-interacting transcription repressor MITR; HDAC7; HDAC7B; HDRP; KIAA0744; MITR; anti-HDAC9 antibody

• Host: Rabbit
• Reactivity: Human, Mouse, Rat
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: HDAC9 polyclonal antibody detects endogenous levels of HDAC9 protein.
• Purity/Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is >95% (by SDS-PAGE).
• Form/Format: Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2
• Sequence Length: 588

• Applicable Applications for anti-HDAC9 antibody: Western Blot (WB)
• Application Notes: WB: 1:500-1:1000
• Immunogen: A synthetic peptide corresponding to residues in Human HDAC9.
• Preparation and Storage: Store at 4 degree C short term.; Aliquot and store at -23 degree C long term.; Avoid freeze-thaw cycles.

Western Blot (WB)

(Western Blot (WB) analysis of HDAC9 polyclonal antibody at 1:500 dilution Lane1:HELA whole cell lysateLane2:RAW264. 7 whole cell lysate Lane3:PC12 whole cell lysate)

Related Product Information for anti-HDAC9 antibody

In the intact cell, DNA closely associates with histones and other nuclear proteins to form chromatin. The remodeling of chromatin is a critical component of transcriptional regulation and the acetylation of nucleosomal histones is a major source of this remodeling. Acetylation of lysine residues in the amino terminal tail domain of histone results in an allosteric change in the nucleosomal conformation and an increased accessibility to transcription factors by DNA. Several mammalian proteins function as nuclear histone acetylases, including GCN5, PCAF (p300/CBP-associated factor), p300/CBP, HAT1 and the TFIID subunit TAF II p250. Conversely, the deacetylation of histones is associated with transcriptional silencing. The histone deacetylases (HDAC) include HDAC1-9. HDAC9 and HDAC9a are two alternatively spliced isoforms of HDAC9. HDAC9a is 132 amino acids shorter than HDAC9, but both isoforms contain the HDAC catalytic domain, remain capable of deacetylase activity and repress myoctye enhancer-binding factor 2-mediated transcription. HDAC9 and HDAC9a are expressed in brain, skeletal muscle, kidney, placenta and pancreas.

NCBI and Uniprot Product Information

• NCBI GI #: 323423044
• NCBI GeneID: 9734
• NCBI Accession #: NP_001191073.1
• NCBI GenBank Nucleotide #: NP_001191073.1
• UniProt Accession #: Q9UKV0
• Molecular Weight: ~ 117kDa
• NCBI Official Full Name: histone deacetylase 9 isoform 6
• NCBI Official Synonym Full Names: histone deacetylase 9
• NCBI Official Symbol: HDAC9
• NCBI Official Synonym Symbols: HD7; HD9; HD7b; HDAC; HDRP; MITR; HDAC7; HDAC7B; HDAC9B; HDAC9FL
• NCBI Protein Information: histone deacetylase 9
• UniProt Protein Name: Histone deacetylase 9
• UniProt Gene Name: HDAC9
• UniProt Synonym Gene Names: HDAC7; HDAC7B; HDRP; KIAA0744; MITR; HD9; HD7; HD7b
• UniProt Entry Name: HDAC9_HUMAN

NCBI Description

Histones play a critical role in transcriptional regulation, cell cycle progression, and developmental events. Histone acetylation/deacetylation alters chromosome structure and affects transcription factor access to DNA. The protein encoded by this gene has sequence homology to members of the histone deacetylase family. This gene is orthologous to the Xenopus and mouse MITR genes. The MITR protein lacks the histone deacetylase catalytic domain. It represses MEF2 activity through recruitment of multicomponent corepressor complexes that include CtBP and HDACs. This encoded protein may play a role in hematopoiesis. Multiple alternatively spliced transcripts have been described for this gene but the full-length nature of some of them has not been determined. [provided by RefSeq, Jul 2008]

Uniprot Description

HDAC9: a transcriptional regulator of the histone deacetylase family, subfamily 2. Deacetylates lysine residues on the N-terminal part of the core histones H2A, H2B, H3 AND H4. Plays an important role in transcriptional regulation, cell cycle progression and developmental events. Histone deacetylases act via the formation of large multiprotein complexes.

Protein type: Transcription, coactivator/corepressor; Deacetylase; EC 3.5.1.98

Chromosomal Location of Human Ortholog: 7p21.1

Cellular Component: nucleoplasm; histone methyltransferase complex; transcription factor complex; histone deacetylase complex; cytoplasm; nucleus

Molecular Function: protein binding; NAD-dependent histone deacetylase activity (H3-K9 specific); protein kinase C binding; NAD-dependent histone deacetylase activity (H3-K14 specific); histone deacetylase binding; metal ion binding; protein deacetylase activity; NAD-dependent histone deacetylase activity (H4-K16 specific); histone deacetylase activity; transcription corepressor activity; transcription factor binding

Biological Process: Notch signaling pathway; regulation of skeletal muscle fiber development; B cell activation; transcription, DNA-dependent; cellular response to insulin stimulus; regulation of striated muscle cell differentiation; B cell differentiation; heart development; histone deacetylation; negative regulation of transcription from RNA polymerase II promoter; inflammatory response; negative regulation of transcription, DNA-dependent