Rabbit anti-Human, Mouse GANP Polyclonal Antibody | anti-GANP antibody

GANP Conjugated Antibody

Cat. Num. AAA9444368

• Gene Names: MCM3AP; GANP; SAC3; MAP80
• Reactivity: Human, Mouse
• Applications: Most Applications
• Synonyms: GANP; Polyclonal Antibody; GANP Conjugated Antibody; 80 kda MCM3-associated protein; GANP protein; KIAA0572; MAP80; MCM3A; anti-GANP antibody

• Host: Rabbit
• Reactivity: Human, Mouse
• Clonality: Polyclonal
• Specificity: The antibody detects endogenous levels of total GANP protein.
• Form/Format: 0.01M Sodium Phosphate, 0.25M NaCl, pH 7.6, 5mg/ml Bovine Serum Albumin, 0.02% Sodium Azide

• Applicable Applications for anti-GANP antibody: Most Applications
• Application Notes: Suggested Dilution:; AF350 conjugated: most applications: 1:50-1:250; AF405 conjugated: most applications: 1:50-1:250; AF488 conjugated: most applications: 1:50-1:250; AF555 conjugated: most applications: 1:50-1:250; AF594 conjugated: most applications: 1:50-1:250; AF647 conjugated: most applications: 1:50-1:250; AF680 conjugated: most applications: 1:50-1:250; AF750 conjugated: most applications: 1:50-1:250; Biotin conjugated: working with enzyme-conjugated streptavidin, most applications: 1:50-1:1000
• Immunogen: Synthesized peptide derived from human GANP.
• Conjugation: Biotin AF350 AF405 AF488 AF555 AF594 AF647 AF680 AF750
• Excitation Emission: AF350: 346nm/442nm
• Preparation and Storage: Store at 4 degree C for 6 months.

Related Product Information for anti-GANP antibody

Isoform GANP:Essential for the generation of high-affinity B-cells against T-cell-dependent antigens by affecting somatic hypermutation at the IgV-regions. May have stimulation-dependent DNA primase activity that would generate extra RNA primers in very rapidely proliferating cells and would support clonal expansion of differentiating B-cells by similarity. Involved in the nuclear export of poly (A)-containing mRNAs by acting as a scaffold for the TREX-2 complex. The TREX-2 complex functions in docking export-competent ribonucleoprotein particles (mRNPs) to the nuclear entrance of the nuclear pore complex (nuclear basket). TREX-2 participates in mRNA export and accurate chromatin positioning in the nucleus by tethering genes to the nuclear periphery. Isoform MCM3AP:Acetyltransferase targeting MCM3. Inhibits initiation of DNA replication, but not elongation.

NCBI and Uniprot Product Information

• NCBI GI #: 19923191
• NCBI GeneID: 8888
• NCBI Accession #: NP_003897.2
• NCBI GenBank Nucleotide #: NM_003906.3
• UniProt Accession #: O60318; Q2M3C1; Q9UMT4
• Molecular Weight: 218,405 Da
• NCBI Official Full Name: 80 kDa MCM3-associated protein
• NCBI Official Synonym Full Names: minichromosome maintenance complex component 3 associated protein
• NCBI Official Symbol: MCM3AP
• NCBI Official Synonym Symbols: GANP; SAC3; MAP80
• NCBI Protein Information: 80 kDa MCM3-associated protein; MCM3 import protein; germinal center-associated nuclear protein; germinal-centre associated nuclear protein; minichromosome maintenance deficient 3-associated protein; MCM3 minichromosome maintenance deficient 3 associated
• UniProt Protein Name: 80 kDa MCM3-associated protein
• Protein Family: Putative arabinogalactan oligomer transport system permease protein
• UniProt Gene Name: MCM3AP
• UniProt Synonym Gene Names: GANP; KIAA0572; MAP80
• UniProt Entry Name: MCM3A_HUMAN

NCBI Description

The minichromosome maintenance protein 3 (MCM3) is one of the MCM proteins essential for the initiation of DNA replication. The protein encoded by this gene is a MCM3 binding protein. It was reported to have phosphorylation-dependent DNA-primase activity, which was up-regulated in antigen immunization induced germinal center. This protein was demonstrated to be an acetyltransferase that acetylates MCM3 and plays a role in DNA replication. The mutagenesis of a nuclear localization signal of MCM3 affects the binding of this protein with MCM3, suggesting that this protein may also facilitate MCM3 nuclear localization. [provided by RefSeq, Jul 2008]

Uniprot Description

GANP: is a member of the SAC3 family of nuclear export factors. In combination with ENY2, PCID2 and centrins, forms the TREX-2 complex that links transcription/ processing with nuclear mRNA export. GANP, required to recruit ENY2 to nuclear pore complexes (NPCs), requires both its CID and MCM3AP domains, together with nucleoporin Nup153, for recruitment to NPCs. The MCM3AP domain, corresponding to the carboxy-terminal 721 amino acids of GANP, shows homology with the Gcn5-related N acetyltransferase superfamily. The protein MCM3AP is encoded entirely within the 3¿ region of the GANP gene. MCM3AP transcription begins within intron 16 of GANP immediately upstream of the first MCM3AP exon, where NF-IL6, AP-1, NF-¿B, and p53 consensus transcription factor binding sites are located. In contrast, the GANP promoter region contains consensus transcription factor binding sites for E2F, E-box, BSAP, and PU.1. MCM3AP interacts with MCM3, which it can acetylate weakly in vivo. GANP is required for efficient mRNA nuclear export: GANP depletion results in the nuclear accumulation of poly(A)+RNA. It interacts via its N-terminal FG repeat domain with the mRNA export factor NXF1. GANP plays a role in the immune response: antibodies from mice deficient for GANP have reduced affinities against T-cell dependent antigens and a lower frequency of variable region somatic mutation. GANP expression is markedly decreased in malignant glioma (MG) with a poor prognosis, consistently lower than that observed in anaplastic astrocytomas. Significant decreases in GANP expression are associated with hyperploidy and chromosomal instability

Protein type: Acetyltransferase; Karyopherin

Chromosomal Location of Human Ortholog: 21q22.3

Cellular Component: nucleoplasm; nuclear membrane; cytoplasm; nuclear pore; cytosol; nucleus

Molecular Function: DNA binding; transferase activity, transferring acyl groups

Biological Process: mRNA transport; immune system process; protein import into nucleus; DNA replication