Human DNA-damage-inducible transcript 4 ELISA Kit | DDIT4 elisa kit

Human DNA damage-inducible transcript 4 protein, DDIT4 ELISA Kit

Cat. Num. AAA944137

• Gene Names: DDIT4; Dig2; REDD1; REDD-1
• Reactivity: Human
• Synonyms: DNA-damage-inducible transcript 4; Human DNA damage-inducible transcript 4 protein; DDIT4 ELISA Kit; Human DNA damage-inducible transcript 4 protein (DDIT4) ELISA kit; Dig2; FLJ20500; REDD-1; REDD1; RP11-442H21.1; RTP801; HIF-1 responsive RTP801 (RTP801) ; DDIT4 elisa kit

• Reactivity: Human
• Specificity: This assay has high sensitivity and excellent specificity for detection of human DDIT4. No significant cross-reactivity or interference between human DDIT4 and analogues was observed.
• Sequence Length: 232

• Samples: Serum, tissue homogenates, cell lysates, urine, cerebrospinal fluid (csf)
• Assay Type: Quantitative Sandwich
• Detection Range: 0.156 ng/ml-10 ng/ml
• Sensitivity: < 0.039 ng/ml
• Intra-assay Precision: Intra-assay Precision (Precision within an assay): CV%<8%. Three samples of known concentration were tested twenty times on one plate to assess.
• Inter-assay Precision: Inter-assay Precision (Precision between assays): CV%<10%. Three samples of known concentration were tested in twenty assays to assess.
• Preparation and Storage: Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for DDIT4 elisa kit

Principle of the Assay: This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for DDIT4 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any DDIT4 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for DDIT4 is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of DDIT4 bound in the initial step. The color development is stopped and the intensity of the color is measured.

NCBI and Uniprot Product Information

• NCBI GI #: 9506687
• NCBI GeneID: 54541
• NCBI Accession #: NP_061931.1
• NCBI GenBank Nucleotide #: NM_019058.2
• UniProt Accession #: Q9NX09; Q9H0S3
• Molecular Weight: 25,371 Da
• NCBI Official Full Name: DNA damage-inducible transcript 4 protein
• NCBI Official Synonym Full Names: DNA-damage-inducible transcript 4
• NCBI Official Symbol: DDIT4
• NCBI Official Synonym Symbols: Dig2; REDD1; REDD-1
• NCBI Protein Information: DNA damage-inducible transcript 4 protein; HIF-1 responsive protein RTP801; protein regulated in development and DNA damage response 1
• UniProt Protein Name: DNA damage-inducible transcript 4 protein
• Protein Family: DNA damage-inducible transcript 4 protein
• UniProt Gene Name: DDIT4
• UniProt Synonym Gene Names: REDD1; RTP801; REDD-1
• UniProt Entry Name: DDIT4_HUMAN

Uniprot Description

DDIT4: Inhibits cell growth by regulating the TOR signaling pathway upstream of the TSC1-TSC2 complex and downstream of AKT1. Promotes neuronal cell death. Up-regulated in fibroblasts upon ionizing radiation, via a TP53-dependent pathway. Up-regulated by TP63 in primary keratinocytes, and down-regulated during keratinocyte differentiation. Up-regulated upon DNA alkylation. Up-regulated by amyloid beta-peptide and retinoic acid. Up-regulated by hypoxia, via a PI3K and HIF1A-dependent but TP53/TP63-independent mechanism. Broadly expressed, with lowest levels in brain, skeletal muscle and intestine. Up-regulated in substantia nigra neurons from Parkinson disease patients. Belongs to the DDIT4 family.

Chromosomal Location of Human Ortholog: 10q22.1

Cellular Component: mitochondrion; cytoplasm; intracellular; cytosol

Biological Process: neuron differentiation; cell proliferation; protein complex disassembly; negative regulation of TOR signaling pathway; nerve growth factor receptor signaling pathway; response to hypoxia; neuron migration; negative regulation of peptidyl-serine phosphorylation; brain development; negative regulation of glycolysis; DNA damage response, signal transduction by p53 class mediator resulting in induction of apoptosis; defense response to virus