Rabbit anti-Human MHC Class I Monoclonal Antibody | anti-HLA-A antibody

MHC Class I Antibody

Cat. Num. AAA9436106

• Gene Names: HLA-A; HLAA
• Reactivity: Human
• Applications: Western Blot, Immunocytochemistry, Immunofluorescence, Immunohistochemistry, Flow Cytometry, Functional Assay
• Purity: ProA affinity purified
• Synonyms: MHC Class I; Monoclonal Antibody; MHC Class I Antibody; A 28 antibody; A 9 antibody; Antigen presenting molecule antibody; Aw 24 antibody; Aw 68 antibody; CLASS I HISTOCOMPATIBILITY ANTIGEN antibody; H2 K1 antibody; H2K antibody; HLA A antibody; HLA class I histocompatibility antigen A 1 alpha chain antibody; HLA class I histocompatibility antigen A 11 alpha chain antibody; HLA class I histocompatibility antigen A 2 alpha chain antibody; HLA class I histocompatibility antigen A 24 alpha chain antibody; HLA class I histocompatibility antigen A 3 alpha chain antibody; HLA class I histocompatibility antigen A 30 alpha chain antibody; HLA class I histocompatibility antigen A 32 alpha chain antibody; HLA class I histocompatibility antigen A 68 alpha chain antibody; HLAA antibody; Leukocyte antigen class I A antibody; Major histocompatibility complex class I A antibody; MHC class I antigen A 1 antibody; MHC class I antigen A*11 antibody; MHC class I antigen A*2 antibody; MHC class I antigen A*24 antibody; MHC class I antigen A*3 antibody; MHC class I antigen A*30 antibody; MHC class I antigen A*32 antibody; MHC class I antigen A*68 antibody; MHC class I antigen HLA A heavy chain antibody; MHC class I heavy chain H2 K antibody; anti-HLA-A antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Monoclonal
• Clone Number: JF10-38
• Purity/Purification: ProA affinity purified
• Form/Format: 1*TBS (pH7.4), 1% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

• Applicable Applications for anti-HLA-A antibody: Western Blot (WB), Immunocytochemistry (ICC), Immunofluorescence (IF), Immunohistochemistry (IHC), Flow Cytometry (FC/FACS)
• Application Notes: WB: 1:1000-5000; IHC: 1:50-1:200; ICC: 1:100-1:500; FC/FACS: 1:50-1:100
• Antibody Type: Recombinant Antibody
• Immunogen: Recombinant protein
• Conjugation: Unconjugated
• Preparation and Storage: Store at -20 degree C.

Western Blot (WB)

(Western blot analysis of MHC class I on different lysates using anti-MHC class I antibody at 1/1, 000 dilution. Positive control: Lane 1: Raji Lane 2: THP-1)

Immunohistochemistry (IHC)

(Immunohistochemical analysis of paraffin-embedded human spleen tissue using anti-MHC class I antibody. Counter stained with hematoxylin.)

Immunocytochemistry (ICC)

(ICC staining MHC class I in SH-SY-5Y cells (red). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.)

Flow Cytometry (FC/FACS)

(Flow cytometric analysis of THP-1 cells with MHC class I antibody at 1/50 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti rabbit IgG was used as the secondary antibody)

Related Product Information for anti-HLA-A antibody

Major histocompatibility complex (MHC) molecules, also designated human leukocyte antigen (HLA) molecules, are cell-surface receptors that bind foreign peptides and present them to T lymphocytes. MHC class I molecules consist of two polypeptide chains, an a or heavy chain, and beta-2-Microglobulin, a non-covalently associated protein. Cytotoxic T lymphocytes bind antigenic peptides presented by MHC class I molecules. Antigens that bind to MHC class I molecules are typically 8-10 residues in length and are stabilized in a peptide binding groove. MHC class II molecules are encoded by polymorphic MHC genes and consist of a non-covalent complex of an a and b chain. Helper T lymphocytes bind antigenic peptides presented by MHC class II molecules. MHC class II molecules bind 13-18 amino acid antigenic peptides. Accumulating in endosomal/lysosomal compartments and on the surface of B cells, HLA-DM and -DO molecules regulate binding of exogenous peptides to class II molecules (HLA-DR) by sustaining a conformation that favors peptide exchange. The differential structural properties of MHC class I and class II molecules account for their respective roles in activating different populations of T lymphocytes.

Product Categories/Family for anti-HLA-A antibody

Total protein Ab

NCBI and Uniprot Product Information

• NCBI GI #: 337752170
• NCBI GeneID: 3105
• NCBI Accession #: NP_001229687.1
• NCBI GenBank Nucleotide #: NM_001242758.1
• UniProt Accession #: P30443; O19673; O19695; O19794; O19795; O43907; O77938; O98010; P10315; P79505; Q9MYA5; Q9MYC4; P01891; P01892; P04439; P05534; P10314; P10316; P13746; P16189; P18462
• Molecular Weight: 41kDa
• NCBI Official Full Name: HLA class I histocompatibility antigen, A-1 alpha chain
• NCBI Official Synonym Full Names: major histocompatibility complex, class I, A
• NCBI Official Symbol: HLA-A
• NCBI Official Synonym Symbols: HLAA
• NCBI Protein Information: HLA class I histocompatibility antigen, A-1 alpha chain; antigen presenting molecule; leukocyte antigen class I-A; MHC class I antigen HLA-A heavy chain
• UniProt Protein Name: HLA class I histocompatibility antigen, A-1 alpha chain
• Protein Family: MHC class II transactivator
• UniProt Gene Name: HLA-A
• UniProt Synonym Gene Names: HLAA

Uniprot Description

Involved in the presentation of foreign antigens to the immune system.