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Western Blot (WB)
(Western blot analysis of Apg7 on Jurkat cells lysates using anti-Apg7 antibody at 1/1, 000 dilution.)
Rabbit anti-Human Apg7 Monoclonal Antibody | anti-Apg7 antibody
Apg7 Antibody
Gene Names
ATG7; GSA7; APG7L; APG7-LIKE
Reactivity
Human
Applications
Western Blot, Immunocytochemistry, Immunofluorescence, Immunohistochemistry, Immunoprecipitation, Flow Cytometry, Functional Assay
Purity
ProA affinity purified
Synonyms
Apg7; Monoclonal Antibody; Apg7 Antibody; 1810013K23Rik antibody; Apg 7 antibody; APG7 autophagy 7 like antibody; APG7 autophagy 7-like (S. cerevisiae) antibody; APG7 like antibody; APG7; S. cerevisiae; homolog of antibody; APG7-like antibody; APG7L antibody; ATG 7 antibody; ATG12-activating enzyme E1 ATG7 antibody; ATG7 antibody; ATG7 autophagy related 7 homolog (S. cerevisiae) antibody; ATG7 autophagy related 7 homolog antibody; ATG7_HUMAN antibody; Atg7l antibody; Autophagy 7; Autophagy related protein 7 antibody; Autophagy-related 7 (yeast) antibody; Autophagy-related protein 7 antibody; DKFZp434N0735 antibody; GSA 7 antibody; GSA7 antibody; hAGP7 antibody; Ubiquitin activating enzyme E1 like protein antibody; Ubiquitin-activating enzyme E1-like protein antibody; Ubiquitin-like modifier-activating enzyme ATG7 antibody; anti-Apg7 antibody
Host
Rabbit
Reactivity
Human
Clonality
Monoclonal
Clone Number
SC06-30
Purity/Purification
ProA affinity purified
Form/Format
1*TBS (pH7.4), 1% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Applicable Applications for anti-Apg7 antibody
Western Blot (WB), Immunocytochemistry (ICC), Immunofluorescence (IF), Immunohistochemistry (IHC), Immunoprecipitation (IP), Flow Cytometry (FC/FACS)
Application Notes
WB: 1:1000-1:2000
IHC: 1:50-1:200
ICC: 1:50-1:200
FC/FACS: 1:50-1:100
IHC: 1:50-1:200
ICC: 1:50-1:200
FC/FACS: 1:50-1:100
Antibody Type
Recombinant Antibody
Immunogen
Recombinant protein
Conjugation
Unconjugated
Preparation and Storage
Store at -20 degree C.
Western Blot (WB)
(Western blot analysis of Apg7 on Jurkat cells lysates using anti-Apg7 antibody at 1/1, 000 dilution.)
Western Blot (WB)
(Western blot analysis of Apg7 on Jurkat cells lysates using anti-Apg7 antibody at 1/1, 000 dilution.)
Immunohistochemistry (IHC)
(Immunohistochemical analysis of paraffin-embedded human tonsil tissue using anti-Apg7 antibody. Counter stained with hematoxylin.)
Immunohistochemistry (IHC)
(Immunohistochemical analysis of paraffin-embedded human tonsil tissue using anti-Apg7 antibody. Counter stained with hematoxylin.)
Immunohistochemistry (IHC)
(Immunohistochemical analysis of paraffin-embedded human spleen tissue using anti-Apg7 antibody. Counter stained with hematoxylin.)
Immunohistochemistry (IHC)
(Immunohistochemical analysis of paraffin-embedded human spleen tissue using anti-Apg7 antibody. Counter stained with hematoxylin.)
Immunohistochemistry (IHC)
(Immunohistochemical analysis of paraffin-embedded human kidney tissue using anti-Apg7 antibody. Counter stained with hematoxylin.)
Immunohistochemistry (IHC)
(Immunohistochemical analysis of paraffin-embedded human kidney tissue using anti-Apg7 antibody. Counter stained with hematoxylin.)
Immunohistochemistry (IHC)
(Immunohistochemical analysis of paraffin-embedded mouse brain tissue using anti-Apg7 antibody. Counter stained with hematoxylin.)
Immunohistochemistry (IHC)
(Immunohistochemical analysis of paraffin-embedded mouse brain tissue using anti-Apg7 antibody. Counter stained with hematoxylin.)
Immunocytochemistry (ICC)
(ICC staining Apg7 in Hela cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.)
Immunocytochemistry (ICC)
(ICC staining Apg7 in Hela cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.)
Immunocytochemistry (ICC)
(ICC staining Apg7 in HepG2 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.)
Immunocytochemistry (ICC)
(ICC staining Apg7 in HepG2 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.)
Flow Cytometry (FC/FACS)
(Flow cytometric analysis of Hela cells with Apg7 antibody at 1/50 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti rabbit IgG was used as the secondary antibody.)
Flow Cytometry (FC/FACS)
(Flow cytometric analysis of Hela cells with Apg7 antibody at 1/50 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti rabbit IgG was used as the secondary antibody.)
Related Product Information for anti-Apg7 antibody
In yeast, autophagy is an essential process for survival during nutrient starvation and cell differentiation. The process of autophagy is characterized as a non-selective degradation of cytoplasmic proteins into membrane stuctures called autophagosomes, and it is dependent on several proteins, including the autophagy proteins APG5 and APG7. Yeast Apg7 and the human homolog, APG7, share similarities with the ubiquitin-activating enzyme E1 in Saccharomyces cerevisiae, and are likewise responsible for enzymatically activating the autophagy conjugation system. Apg5 and the human homolog, APG5 (also designated apoptosis specific protein or APS), function as substrates for the autophagy protein APG12. These proteins are covalently bonded together to form APG12/APG5 conjugates, which are required for the progression of autophagy.
Product Categories/Family for anti-Apg7 antibody
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
703
NCBI Official Full Name
ubiquitin-like modifier-activating enzyme ATG7 isoform b
NCBI Official Synonym Full Names
autophagy related 7
NCBI Official Symbol
ATG7
NCBI Official Synonym Symbols
GSA7; APG7L; APG7-LIKE
NCBI Protein Information
ubiquitin-like modifier-activating enzyme ATG7; hAGP7; autophagy-related protein 7; ATG12-activating enzyme E1 ATG7; ATG7 autophagy related 7 homolog; ubiquitin activating enzyme E1-like protein; ubiquitin-activating enzyme E1-like protein
UniProt Protein Name
Ubiquitin-like modifier-activating enzyme ATG7
UniProt Gene Name
ATG7
UniProt Synonym Gene Names
APG7L; APG7-like; hAGP7
UniProt Entry Name
ATG7_HUMAN
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Product Notes
The Apg7 atg7 (Catalog #AAA9435759) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Apg7 Antibody reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's Apg7 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunocytochemistry (ICC), Immunofluorescence (IF), Immunohistochemistry (IHC), Immunoprecipitation (IP), Flow Cytometry (FC/FACS). WB: 1:1000-1:2000 IHC: 1:50-1:200 ICC: 1:50-1:200 FC/FACS: 1:50-1:100. Researchers should empirically determine the suitability of the Apg7 atg7 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Apg7, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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