Rabbit beta 2 Microglobulin Monoclonal Antibody | anti-B2M antibody

beta 2 Microglobulin Antibody

Cat. Num. AAA9435650

• Reactivity: Human, Mouse, Rat
• Applications: Western Blot, Immunocytochemistry, Immunoprecipitation
• Purity: ProA affinity purified
• Synonyms: beta 2 Microglobulin; Monoclonal Antibody; beta 2 Microglobulin Antibody; B2M antibody; B2MG_HUMAN antibody; Beta 2 microglobin antibody; Beta 2 microglobulin antibody; Beta 2 microglobulin precursor antibody; Beta chain of mhc class 1 proteins antibody; Beta chain of MHC class I molecules antibody; Beta-2-microglobulin form pI 5.3 antibody; CDABP0092 antibody; Hdcma22p antibody; anti-B2M antibody

• Host: Rabbit
• Reactivity: Human, Mouse, Rat
• Clonality: Monoclonal
• Clone Number: SU35-05
• Purity/Purification: ProA affinity purified
• Form/Format: 1*TBS (pH7.4), 1% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

• Applicable Applications for anti-B2M antibody: Western Blot (WB), Immunocytochemistry (ICC), Immunoprecipitation (IP)
• Application Notes: WB: 1:1000-1:2000; ICC: 1:50-1:200
• Antibody Type: Recombinant Antibody
• Immunogen: Recombinant protein
• Conjugation: Unconjugated
• Preparation and Storage: Store at -20 degree C.

Western Blot (WB)

(Western blot analysis of beta 2 Microglobulin on different lysates using anti-beta 2 Microglobulin antibody at 1/1, 000 dilution. Positive control: Lane 1: Raji Lane 2: U937)

Immunocytochemistry (ICC)

(ICC staining beta 2 Microglobulin in Hela cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.)

Immunocytochemistry (ICC)

(ICC staining beta 2 Microglobulin in A431 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.)

Related Product Information for anti-B2M antibody

Major histocompatibility complex (MHC) class 1 molecules bind to antigens for presentation on the surface of cells. The proteasome is responsible for producing these antigens from the components of foreign pathogens. MHC class 1 molecules consist of an a heavy chain that contains three subdomains (alpha1, alpha2, alpha3), and a non-covalent associating light chain, known as beta-2-Microglobulin. beta-2-Microglobulin associates with the a3 subdomain of the a heavy chain and forms an immunoglobulin domain-like structure that mediates proper folding and expression of MHC class 1 molecules. The alpha1 and alpha2 domains of the a heavy chain form the peptide antigen-binding cleft. Mice that lack beta-2-Microglobulin protein show a normal distribution of T cells, yet have no mature CD4-8+ T cells and are defective in CD4-8+ T cell-mediated cytotoxicity. Interferon-gamma can stimulate production of beta-2-Microglobulin transcripts. The human beta-2-Microglobulin gene maps to chromosome 15q21-q22.2 and encodes a 119 amino acid protein. Mutations in the beta-2-Microglobulin gene can enhance the progression of malignant melanoma phenotypes.

Product Categories/Family for anti-B2M antibody

Total protein Ab

NCBI and Uniprot Product Information

• NCBI GI #: 48428791
• NCBI GeneID: 567
• UniProt Accession #: P61769; P01884; Q540F8; Q6IAT8; Q9UCK0; Q9UD48; Q9UDF4
• Molecular Weight: 119
• NCBI Official Full Name: Beta-2-microglobulin
• NCBI Official Synonym Full Names: beta-2-microglobulin
• NCBI Official Symbol: B2M
• NCBI Protein Information: beta-2-microglobulin; beta-2-microglobin; beta chain of MHC class I molecules
• UniProt Protein Name: Beta-2-microglobulin
• Protein Family: Beta-2-microglobulin
• UniProt Gene Name: B2M
• UniProt Entry Name: B2MG_HUMAN

NCBI Description

This gene encodes a serum protein found in association with the major histocompatibility complex (MHC) class I heavy chain on the surface of nearly all nucleated cells. The protein has a predominantly beta-pleated sheet structure that can form amyloid fibrils in some pathological conditions. The encoded antimicrobial protein displays antibacterial activity in amniotic fluid. A mutation in this gene has been shown to result in hypercatabolic hypoproteinemia.[provided by RefSeq, Aug 2014]

Uniprot Description

B2M: Component of the class I major histocompatibility complex (MHC). Involved in the presentation of peptide antigens to the immune system. Heterodimer of an alpha chain and a beta chain. Beta-2- microglobulin is the beta-chain of major histocompatibility complex class I molecules. Polymers of beta 2-microglobulin can be found in tissues from patients on long-term hemodialysis. Belongs to the beta-2-microglobulin family.

Protein type: Secreted; Secreted, signal peptide

Chromosomal Location of Human Ortholog: 15q21.1

Cellular Component: Golgi membrane; Golgi apparatus; extracellular space; phagocytic vesicle membrane; focal adhesion; membrane; early endosome membrane; endoplasmic reticulum lumen; cytoplasm; plasma membrane; extracellular region; MHC class I protein complex; external side of plasma membrane

Molecular Function: identical protein binding; protein binding

Biological Process: response to drug; regulation of immune response; viral reproduction; positive regulation of T cell mediated cytotoxicity; antigen processing and presentation of exogenous peptide antigen via MHC class I, TAP-independent; cytokine and chemokine mediated signaling pathway; T cell differentiation in the thymus; antigen processing and presentation of exogenous peptide antigen via MHC class I, TAP-dependent; regulation of defense response to virus by virus; retinal homeostasis; iron ion homeostasis; antigen processing and presentation of peptide antigen via MHC class I; response to cadmium ion; response to molecule of bacterial origin; antigen processing and presentation of exogenous peptide antigen via MHC class I; innate immune response; protein refolding; antigen processing and presentation of exogenous protein antigen via MHC class Ib, TAP-dependent

Disease: Hypoproteinemia, Hypercatabolic