Rabbit EYA3 Polyclonal Antibody | anti-EYA3 antibody

EYA3 Polyclonal Antibody

Cat. Num. AAA9434202

• Reactivity: Human, Mouse, Rat
• Applications: Western Blot, Immunohistochemistry
• Purity: Affinity purification
• Synonyms: EYA3; Polyclonal Antibody; EYA3 Polyclonal Antibody; eyes absent homolog 3; anti-EYA3 antibody

• Host: Rabbit
• Reactivity: Human, Mouse, Rat
• Clonality: Polyclonal
• Isotype: IgG
• Purity/Purification: Affinity purification
• Form/Format: PBS with 50% glycerol, pH7.4

• Applicable Applications for anti-EYA3 antibody: Western Blot (WB), Immunohistochemistry (IHC)
• Application Notes: WB: 1:500-1:2000; IHC: 1:50-1:200
• Immunogen: Recombinant fusion protein of human EYA3 (NP_001981.2).
• Conjugation: Unconjugated
• Preparation and Storage: Store at -20 degree C. Avoid freeze/thaw cycles.

Western Blot (WB)

(Western blot analysis of extracts of various cell lines, using EYA3 antibody.)

Immunohistochemistry (IHC)

(Immunohistochemistry of paraffin-embedded rat kidney using EYA3 antibody.)

Immunohistochemistry (IHC)

(Immunohistochemistry of paraffin-embedded human kidney using EYA3 antibody.)

Immunohistochemistry (IHC)

(Immunohistochemistry of paraffin-embedded human stomach using EYA3 antibody.)

Immunohistochemistry (IHC)

(Immunohistochemistry of paraffin-embedded mouse liver using EYA3 antibody.)

Related Product Information for anti-EYA3 antibody

This gene encodes a member of the eyes absent (EYA) family of proteins. The encoded protein may act as a transcriptional activator and have a role during development. It can act as a mediator of chemoresistance and cell survival in Ewing sarcoma cells, where this gene is up-regulated via a micro-RNA that binds to the 3' UTR of the transcript. A similar protein in mice acts as a transcriptional activator. Alternative splicing of this gene results in multiple transcript variants.

Product Categories/Family for anti-EYA3 antibody

Total protein Ab

NCBI and Uniprot Product Information

• NCBI GI #: 26667243
• NCBI GeneID: 2140
• NCBI Accession #: NP_001981.2
• NCBI GenBank Nucleotide #: NM_001990.3
• UniProt Accession #: Q99504; O95463; Q8IVX7; Q99813; A8K190; B4DIR7; B4DNZ7
• Molecular Weight: 57,827 Da
• NCBI Official Full Name: eyes absent homolog 3 isoform a
• NCBI Official Synonym Full Names: EYA transcriptional coactivator and phosphatase 3
• NCBI Official Symbol: EYA3
• NCBI Protein Information: eyes absent homolog 3; eyes absent 3
• UniProt Protein Name: Eyes absent homolog 3
• Protein Family: Eyes absent
• UniProt Gene Name: EYA3
• UniProt Entry Name: EYA3_HUMAN

NCBI Description

This gene encodes a member of the eyes absent (EYA) family of proteins. The encoded protein may act as a transcriptional activator and have a role during development. It can act as a mediator of chemoresistance and cell survival in Ewing sarcoma cells, where this gene is up-regulated via a micro-RNA that binds to the 3' UTR of the transcript. A similar protein in mice acts as a transcriptional activator. Alternative splicing of this gene results in multiple transcript variants. [provided by RefSeq, Sep 2013]

Uniprot Description

EYA3: Tyrosine phosphatase that specifically dephosphorylates 'Tyr-142' of histone H2AX (H2AXY142ph). 'Tyr-142' phosphorylation of histone H2AX plays a central role in DNA repair and acts as a mark that distinguishes between apoptotic and repair responses to genotoxic stress. Promotes efficient DNA repair by dephosphorylating H2AX, promoting the recruitment of DNA repair complexes containing MDC1. Its function as histone phosphatase probably explains its role in transcription regulation during organogenesis. Coactivates SIX1, and seems to coactivate SIX2, SIX4 and SIX5. The repression of precursor cell proliferation in myoblasts by SIX1 is switched to activation through recruitment of EYA3 to the SIX1-DACH1 complex and seems to be dependent on EYA3 phosphatase activity. May be involved in development of the eye. Belongs to the HAD-like hydrolase superfamily. EYA family. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: EC 3.1.3.48; Protein phosphatase, tyrosine (non-receptor)

Chromosomal Location of Human Ortholog: 1p36

Cellular Component: centrosome; cytoplasm; nucleoplasm; nucleus; transcription factor complex

Molecular Function: metal ion binding; protein binding; protein tyrosine phosphatase activity

Biological Process: anatomical structure morphogenesis; double-strand break repair; histone dephosphorylation; multicellular organismal development; positive regulation of DNA repair; regulation of transcription, DNA-dependent; response to ionizing radiation; transcription, DNA-dependent; visual perception