Western Blot (WB)
(Western blot analysis of extracts from normal (control) and LIG4 knockout (KO) HeLa cells, using LIG4 antibody.)
Rabbit anti-Human, Mouse LIG4 Polyclonal Antibody | anti-LIG4 antibody
LIG4 Polyclonal Antibody
IF: 1:10-1:100
Western Blot (WB)
(Western blot analysis of extracts from normal (control) and LIG4 knockout (KO) HeLa cells, using LIG4 antibody.)
Western Blot (WB)
(Western blot analysis of extracts from normal (control) and LIG4 knockout (KO) HeLa cells, using LIG4 antibody.)
NCBI and Uniprot Product Information
NCBI Description
The protein encoded by this gene is a DNA ligase that joins single-strand breaks in a double-stranded polydeoxynucleotide in an ATP-dependent reaction. This protein is essential for V(D)J recombination and DNA double-strand break (DSB) repair through nonhomologous end joining (NHEJ). This protein forms a complex with the X-ray repair cross complementing protein 4 (XRCC4), and further interacts with the DNA-dependent protein kinase (DNA-PK). Both XRCC4 and DNA-PK are known to be required for NHEJ. The crystal structure of the complex formed by this protein and XRCC4 has been resolved. Defects in this gene are the cause of LIG4 syndrome. Alternatively spliced transcript variants encoding the same protein have been observed. [provided by RefSeq, Jul 2008]
Uniprot Description
LIG4: a DNA ligase that joins single-strand breaks in a double-stranded polydeoxynucleotide in an ATP-dependent reaction. Essential for V(D)J recombination and DNA double-strand break (DSB) repair through nonhomologous end joining (NHEJ). This protein forms a complex with the X-ray repair cross complementing protein 4 (XRCC4), and further interacts with the DNA-dependent protein kinase (DNA-PK). Both XRCC4 and DNA-PK are known to be required for NHEJ. Defects in this gene are the cause of LIG4 syndrome.
Protein type: EC 6.5.1.1; Ligase
Chromosomal Location of Human Ortholog: 13q33-q34
Cellular Component: nucleoplasm; DNA ligase IV complex; focal adhesion; cytoplasm; plasma membrane; condensed chromosome; DNA-dependent protein kinase complex; nucleus
Molecular Function: protein C-terminus binding; protein binding; DNA binding; DNA ligase activity; metal ion binding; DNA ligase (ATP) activity; ATP binding; ligase activity
Biological Process: central nervous system development; viral reproduction; somatic stem cell maintenance; T cell differentiation in the thymus; chromosome organization and biogenesis; double-strand break repair via nonhomologous end joining; DNA ligation; positive regulation of fibroblast proliferation; double-strand break repair; DNA ligation during DNA repair; response to gamma radiation; negative regulation of neuron apoptosis; DNA ligation during DNA recombination; response to X-ray; pro-B cell differentiation; single strand break repair; positive regulation of neurogenesis; V(D)J recombination; immunoglobulin V(D)J recombination; in utero embryonic development; isotype switching; cell cycle; DNA repair; lagging strand elongation; cell proliferation; neuron apoptosis; cell division; T cell receptor V(D)J recombination; nucleotide-excision repair, DNA gap filling
Disease: Myeloma, Multiple; Lig4 Syndrome
Research Articles on LIG4
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Product Notes
The LIG4 lig4 (Catalog #AAA9433407) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The LIG4 Polyclonal Antibody reacts with Human, Mouse and may cross-react with other species as described in the data sheet. AAA Biotech's LIG4 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunofluorescence (IF). WB: 1:500-1:2000 IF: 1:10-1:100. Researchers should empirically determine the suitability of the LIG4 lig4 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "LIG4, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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