Human Macrophage metalloelastase ELISA Kit | MMP12 elisa kit

Human Macrophage metalloelastase ELISA Kit

Cat. Num. AAA9425578

• Gene Names: MMP12; ME; HME; MME; MMP-12
• Reactivity: Human
• Synonyms: Macrophage metalloelastase; Human Macrophage metalloelastase ELISA Kit; MME; Macrophage elastase; ME; hME; Matrix metalloproteinase-12; MMP-12; MMP12; HME; 3.4.24.65; MMP12 elisa kit

• Reactivity: Human
• Specificity: This assay recognizes recombinant and natural human MME. No significant cross-reactivity or interference was observed.

• Samples: Serum, plasma, tissue homogenates and cell culture supernates and other biological fluids
• Detection Range: 0.15 ng/mL -10.0 ng/mL
• Sensitivity: < 0.039 ng/mL
• Preparation and Storage: Store at 4 degree C

Typical Testing Data/Standard Curve (for reference only)

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Related Product Information for MMP12 elisa kit

Intended Uses: This immunoassay kit allows for the in vitro quantitative determination of human macrophage metalloelastase,MME concentrations in serum, Plasma, tissue homogenates and Cell culture supernates and Other biological fluids.

Principle of the Assay: The microtiter plate provided in this kit has been pre-coated with an antibody specific to MME. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated polyclonal antibody preparation specific for MME and Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain MME, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm +/- 2 nm. The concentration of MME in the samples is then determined by comparing the O.D. of the samples to the standard curve.

NCBI and Uniprot Product Information

• NCBI GI #: 73858572
• NCBI GeneID: 4321
• NCBI Accession #: NP_002417.2
• NCBI GenBank Nucleotide #: NM_002426.5
• UniProt Accession #: P39900; Q2M1L9; B2R9X8; B7ZLF6
• Molecular Weight: 54,002 Da
• NCBI Official Full Name: macrophage metalloelastase preproprotein
• NCBI Official Synonym Full Names: matrix metallopeptidase 12
• NCBI Official Symbol: MMP12
• NCBI Official Synonym Symbols: ME; HME; MME; MMP-12
• NCBI Protein Information: macrophage metalloelastase
• UniProt Protein Name: Macrophage metalloelastase
• Protein Family: Macrophage metalloelastase
• UniProt Gene Name: MMP12
• UniProt Synonym Gene Names: HME; MME; ME; hME; MMP-12

NCBI Description

This gene encodes a member of the peptidase M10 family of matrix metalloproteinases (MMPs). Proteins in this family are involved in the breakdown of extracellular matrix in normal physiological processes, such as embryonic development, reproduction, and tissue remodeling, as well as in disease processes, such as arthritis and metastasis. The encoded preproprotein is proteolytically processed to generate the mature protease. This protease degrades soluble and insoluble elastin. This gene may play a role in aneurysm formation and mutations in this gene are associated with lung function and chronic obstructive pulmonary disease (COPD). This gene is part of a cluster of MMP genes on chromosome 11. [provided by RefSeq, Jan 2016]

Uniprot Description

MMP12: May be involved in tissue injury and remodeling. Has significant elastolytic activity. Can accept large and small amino acids at the P1' site, but has a preference for leucine. Aromatic or hydrophobic residues are preferred at the P1 site, with small hydrophobic residues (preferably alanine) occupying P3. Belongs to the peptidase M10A family.

Protein type: EC 3.4.24.65; Protease; Secreted; Secreted, signal peptide

Chromosomal Location of Human Ortholog: 11q22.2

Cellular Component: cytoplasm; extracellular region; extracellular space; nucleus

Molecular Function: calcium ion binding; collagen binding; endopeptidase activity; metalloendopeptidase activity; sequence-specific DNA binding; serine-type endopeptidase activity; zinc ion binding

Biological Process: collagen catabolic process; extracellular matrix disassembly; positive regulation of epithelial cell proliferation involved in wound healing; positive regulation of transcription from RNA polymerase II promoter; protein import into nucleus; proteolysis; wound healing, spreading of epidermal cells