Rabbit anti-Human DOK1 Polyclonal Antibody | anti-DOK1 antibody

DOK1 Antibody

Cat. Num. AAA9413453

• Gene Names: DOK1; P62DOK
• Reactivity: Human
• Applications: ELISA, Western Blot, Immunocytochemistry
• Purity: Affinity chromatography purified via peptide column
• Synonyms: DOK1; Polyclonal Antibody; DOK1 Antibody; anti-DOK1 antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Purity/Purification: Affinity chromatography purified via peptide column
• Form/Format: Supplied in PBS containing 0.02% sodium azide.
• Sequence Length: 481

• Applicable Applications for anti-DOK1 antibody: ELISA (EIA), Western Blot (WB), Immunocytochemistry (ICC)
• Immunogen Type: Peptide
• Immunogen Description: Raised against a peptide corresponding to amino acids near the carboxy terminus of human DOK1.
• Target Name: DOK1
• Preparation and Storage: Can be stored at -20 degree C, stable for one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

Western Blot (WB)

(Western blot analysis of DOK1 in Jurkat (Jur) and THP-1 (THP) cell lysates with DOK1 antibody at 1 ug/mL.)

Immunocytochemistry (ICC)

(Immunocytochemistry of DOK1 in K562 cells with DOK1 antibody at 2 ug/mL.)

Related Product Information for anti-DOK1 antibody

Signals from most growth factors and cytokines are transduced by receptor tyrosine kinases or non-receptor tyrosine kinases. Activated tyrosine kinases phosphorylate their substrates, which mediate the cellular response to extracellular stimuli. A long-sought major substrate termed p62dok (downstream of tyrosine kinase) for many tyrosine kinases including c-kit, v-abl, v-Fps, v-Src, v-Fms, and activated EGF, PDGF, IGF, VEGF and insulin receptors was identified recently from human and mouse by several laboratories. Upon phosphorylation, p62dok forms a complex with the ras GTPase-activating protein (RasGAP). p62dok represents a new family with very recently identified p56dok.

Product Categories/Family for anti-DOK1 antibody

Total protein Ab

NCBI and Uniprot Product Information

• NCBI GI #: 1794261
• NCBI GeneID: 1796
• Molecular Weight: 37,405 Da
• NCBI Official Full Name: GAP binding protein p62dok
• NCBI Official Synonym Full Names: docking protein 1, 62kDa (downstream of tyrosine kinase 1)
• NCBI Official Symbol: DOK1
• NCBI Official Synonym Symbols: P62DOK
• NCBI Protein Information: docking protein 1
• UniProt Protein Name: Docking protein 1
• Protein Family: Docking protein
• UniProt Gene Name: DOK1
• UniProt Entry Name: DOK1_HUMAN

NCBI Description

The protein encoded by this gene is part of a signal transduction pathway downstream of receptor tyrosine kinases. The encoded protein is a scaffold protein that helps form a platform for the assembly of multiprotein signaling complexes. Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Oct 2010]

Uniprot Description

DOK1: a docking protein that interacts with receptor tyrosine kinases. It is constitutively tyrosine phosphorylated in hematopoietic progenitors isolated from chronic myelogenous leukemia (CML) patients in the chronic phase. It may be a critical substrate for p210(bcr/abl), a chimeric protein whose presence is associated with CML. Docking protein 1 contains a putative pleckstrin homology domain at the amino terminus and ten PXXP SH3 recognition motifs. Docking protein 2 binds p120 (RasGAP) from CML cells. It has been postulated to play a role in mitogenic signaling.

Protein type: Adaptor/scaffold; Motility/polarity/chemotaxis

Chromosomal Location of Human Ortholog: 2p13

Cellular Component: perinuclear region of cytoplasm; cytoplasm; nucleus; cytosol

Molecular Function: protein binding; receptor signaling protein activity; insulin receptor binding

Biological Process: cell surface receptor linked signal transduction; Ras protein signal transduction; insulin receptor signaling pathway; signal transduction; transmembrane receptor protein tyrosine kinase signaling pathway