Testing Data
(Gel: 6%SDS-PAGE Lysate: 40ug Raji cell Primary antibody: 1/350 dilution Secondary antibody dilution: 1/8000 Exposure time: 10 seconds)
Rabbit anti-Human COPB1 Polyclonal Antibody | anti-COPB1 antibody
COPB1 Antibody
Immunohistochemistry: 1:50-1:200
Testing Data
(Gel: 6%SDS-PAGE Lysate: 40ug Raji cell Primary antibody: 1/350 dilution Secondary antibody dilution: 1/8000 Exposure time: 10 seconds)
Testing Data
(Gel: 6%SDS-PAGE Lysate: 40ug Raji cell Primary antibody: 1/350 dilution Secondary antibody dilution: 1/8000 Exposure time: 10 seconds)
Immunohistochemistry (IHC)
(Immunohistochemical analysis of paraffin-embedded Human thyroid cancer tissue using at dilution 1/50.)
Immunohistochemistry (IHC)
(Immunohistochemical analysis of paraffin-embedded Human thyroid cancer tissue using at dilution 1/50.)
NCBI and Uniprot Product Information
NCBI Description
This gene encodes a protein subunit of the coatomer complex associated with non-clathrin coated vesicles. The coatomer complex, also known as the coat protein complex 1, forms in the cytoplasm and is recruited to the Golgi by activated guanosine triphosphatases. Once at the Golgi membrane, the coatomer complex may assist in the movement of protein and lipid components back to the endoplasmic reticulum. Alternatively spliced transcript variants have been described. [provided by RefSeq, Jan 2009]
Uniprot Description
COPB1: The coatomer is a cytosolic protein complex that binds to dilysine motifs and reversibly associates with Golgi non- clathrin-coated vesicles, which further mediate biosynthetic protein transport from the ER, via the Golgi up to the trans Golgi network. Coatomer complex is required for budding from Golgi membranes, and is essential for the retrograde Golgi-to-ER transport of dilysine-tagged proteins. In mammals, the coatomer can only be recruited by membranes associated to ADP-ribosylation factors (ARFs), which are small GTP-binding proteins; the complex also influences the Golgi structural integrity, as well as the processing, activity, and endocytic recycling of LDL receptors. Plays a functional role in facilitating the transport of kappa- type opioid receptor mRNAs into axons and enhances translation of these proteins. Required for limiting lipid storage in lipid droplets. Involved in lipid homeostasis by regulating the presence of perilipin family members PLIN2 and PLIN3 at the lipid droplet surface and promoting the association of adipocyte surface triglyceride lipase (PNPLA2) with the lipid droplet to mediate lipolysis. Involved in the Golgi disassembly and reassembly processes during cell cycle. Involved in autophagy by playing a role in early endosome function. Plays a role in organellar compartmentalization of secretory compartments including endoplasmic reticulum (ER)-Golgi intermediate compartment (ERGIC), Golgi, trans-Golgi network (TGN) and recycling endosomes, and in biosynthetic transport of CAV1. Promotes degradation of Nef cellular targets CD4 and MHC class I antigens by facilitating their trafficking to degradative compartments.
Protein type: Vesicle; Motility/polarity/chemotaxis
Chromosomal Location of Human Ortholog: 11p15.2
Cellular Component: Golgi membrane; Golgi apparatus; intracellular membrane-bound organelle; membrane; cytoplasm; ER-Golgi intermediate compartment; plasma membrane; Golgi-associated vesicle; COPI vesicle coat; cytosol
Molecular Function: protein binding; structural molecule activity
Biological Process: intra-Golgi vesicle-mediated transport; intracellular protein transport; COPI coating of Golgi vesicle; viral reproduction; retrograde vesicle-mediated transport, Golgi to ER
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Product Notes
The COPB1 copb1 (Catalog #AAA9412428) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The COPB1 Antibody reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's COPB1 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunohistochemistry (IHC). Western blotting: 1:200-1:1000 Immunohistochemistry: 1:50-1:200. Researchers should empirically determine the suitability of the COPB1 copb1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "COPB1, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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