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Testing Data (Gel: 8%SDS-PAGE Lysates (from left to right): HepG2, Raji, A431 and 231 cell Amount of lysate: 40ug per lane Primary antibody: 1/800 dilution Secondary antibody dilution: 1/8000 Exposure time: 5 seconds)

Rabbit anti-Human DNAJA1 Polyclonal Antibody | anti-DNAJA1 antibody

DNAJA1 Antibody

Gene Names
DNAJA1; DJ-2; DjA1; HDJ2; HSDJ; HSJ2; HSPF4; NEDD7; hDJ-2
Reactivity
Human
Applications
Western Blot, Immunohistochemistry
Purity
Antigen affinity purification.
Synonyms
DNAJA1; Polyclonal Antibody; DNAJA1 Antibody; DJ-2; DjA1; HDJ2; HSDJ; HSJ2; HSPF4; NEDD7; hDJ-2; anti-DNAJA1 antibody
Ordering
For Research Use Only!
Host
Rabbit
Reactivity
Human
Clonality
Polyclonal
Specificity
The antibody detects endogenous levels of total DNAJA1 protein.
Purity/Purification
Antigen affinity purification.
Form/Format
Rabbit IgG in pH7.3 PBS, 0.05% NaN3, 50% Glycerol.
Concentration
2.8 mg/ml (varies by lot)
Sequence Length
397
Applicable Applications for anti-DNAJA1 antibody
Western Blot (WB), Immunohistochemistry (IHC)
Application Notes
Western blotting: 1:500-1:2000
Immunohistochemistry: 1:100-1:300
Immunogen Type
Recombinant Protein
Immunogen Description
Fusion protein corresponding to a region derived from internal residues of human DnaJ (Hsp40) homolog, subfamily A, member 1
Target Name
DNAJA1
Preparation and Storage
Store at -20 degree C

Testing Data

(Gel: 8%SDS-PAGE Lysates (from left to right): HepG2, Raji, A431 and 231 cell Amount of lysate: 40ug per lane Primary antibody: 1/800 dilution Secondary antibody dilution: 1/8000 Exposure time: 5 seconds)

Testing Data (Gel: 8%SDS-PAGE Lysates (from left to right): HepG2, Raji, A431 and 231 cell Amount of lysate: 40ug per lane Primary antibody: 1/800 dilution Secondary antibody dilution: 1/8000 Exposure time: 5 seconds)

Immunohistochemistry (IHC)

(Immunohistochemical analysis of paraffin-embedded Human colon cancer tissue using at dilution 1/60.)

Immunohistochemistry (IHC) (Immunohistochemical analysis of paraffin-embedded Human colon cancer tissue using at dilution 1/60.)
Related Product Information for anti-DNAJA1 antibody
DnaJ-like proteins interact with HSP 70 molecular chaperones and function to facilitate protein folding and mitochondrial protein import. HSP 40-4, also known as HDJ2, is the human DnaJ homolog that functions as a co-chaperone with a cysteine-rich zinc finger domain. The cellular redox enzyme thioredoxin interacts with HSP 40-4, and oxidation and reduction reversibly regulate HSP 40-4 function in response to the changing redox states of the cell. The zinc finger domain of HSP 40-4 may act as a redox sensor of chaperone-mediated protein-folding machinery, since HSP 40-4 inactivation leads to the oxidation of cysteine thiols and a simultaneous release of coordinated zinc. Loss of the HSP 40-4 protein may be linked to severe defects in spermatogenesis that involve aberrant androgen signaling.
Product Categories/Family for anti-DNAJA1 antibody

NCBI and Uniprot Product Information

NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
Molecular Weight
37,045 Da
NCBI Official Full Name
dnaJ homolog subfamily A member 1
NCBI Official Synonym Full Names
DnaJ (Hsp40) homolog, subfamily A, member 1
NCBI Official Symbol
DNAJA1
NCBI Official Synonym Symbols
DJ-2; DjA1; HDJ2; HSDJ; HSJ2; HSPF4; NEDD7; hDJ-2
NCBI Protein Information
dnaJ homolog subfamily A member 1
UniProt Protein Name
DnaJ homolog subfamily A member 1
Protein Family
UniProt Gene Name
DNAJA1
UniProt Synonym Gene Names
DNAJ2; HDJ2; HSJ2; HSPF4; HSJ-2; hDj-2
UniProt Entry Name
DNJA1_HUMAN

Uniprot Description

DNAJA1: Co-chaperone of Hsc70. Seems to play a role in protein import into mitochondria.

Protein type: Nuclear receptor co-regulator; Chaperone

Chromosomal Location of Human Ortholog: 9p13.3

Cellular Component: membrane; mitochondrion; perinuclear region of cytoplasm; nucleus; cytosol; ubiquitin ligase complex

Molecular Function: protein binding; G-protein-coupled receptor binding; low-density lipoprotein receptor binding; chaperone binding; ubiquitin protein ligase binding; metal ion binding; unfolded protein binding; Hsp70 protein binding; ATP binding

Biological Process: regulation of protein transport; negative regulation of JNK activity; sperm motility; protein folding; positive regulation of apoptosis; response to heat; androgen receptor signaling pathway; negative regulation of protein ubiquitination; spermatogenesis; DNA damage response, detection of DNA damage; protein refolding; response to unfolded protein; negative regulation of apoptosis

Research Articles on DNAJA1

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Product Notes

The DNAJA1 dnaja1 (Catalog #AAA9412247) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The DNAJA1 Antibody reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's DNAJA1 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunohistochemistry (IHC). Western blotting: 1:500-1:2000 Immunohistochemistry: 1:100-1:300. Researchers should empirically determine the suitability of the DNAJA1 dnaja1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "DNAJA1, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.

Precautions

All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.

Disclaimer

Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.

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