Rabbit G6PD Polyclonal Antibody | anti-G6PD antibody

G6PD Antibody

Cat. Num. AAA9411549

• Gene Names: G6PD; G6PD1
• Reactivity: Human, Mouse, Rat
• Applications: Western Blot, Immunohistochemistry
• Purity: Antibodies were purified by affinity purification using immunogen.
• Synonyms: G6PD; Polyclonal Antibody; G6PD Antibody; G6PD1; anti-G6PD antibody

• Host: Rabbit
• Reactivity: Human, Mouse, Rat
• Clonality: Polyclonal
• Specificity: The antibody detects endogenous level of total G6PD protein.
• Purity/Purification: Antibodies were purified by affinity purification using immunogen.
• Form/Format: Supplied at 1.0mg/mL in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
• Concentration: 1.0 mg/ml (varies by lot)
• Sequence Length: 545

• Applicable Applications for anti-G6PD antibody: Western Blot (WB), Immunohistochemistry (IHC)
• Application Notes: Western blotting: 1:500 - 1:2000; Immunohistochemistry: 1:50 - 1:100
• Immunogen Type: Recombinant Protein
• Immunogen Description: Recombinant protein of human G6PD.
• Target Name: G6PD
• Preparation and Storage: Store at -20 degree C

Western Blot (WB)

(Western blot analysis of extracts of various cell lines, using G6PD antibody.)

Immunohistochemistry (IHC)

(Immunohistochemical analysis of paraffin-embedded human esophageal cancer using G6PD antibody at dilution of 1:100 (400x lens).)

Related Product Information for anti-G6PD antibody

Glucose-6-phosphate dehydrogenase (G6PD) catalyses the first and rate-limiting step of the pentose phosphate pathway (1). The NADPH generated from this reaction is essential to protect cells from oxidative stress (1). Recent studies have shown that p53 interacts with G6PD and inhibits its activity, therefore suppressing glucose consumption through the pentose phosphate pathway (2). In cancer cells with p53 mutations, the increased glucose consumption is directed towards increased biosynthesis, which is critical for cancer cell proliferation (2).

Product Categories/Family for anti-G6PD antibody

Total protein Ab

NCBI and Uniprot Product Information

• NCBI GI #: 109389365
• NCBI GeneID: 2539
• NCBI Accession #: NP_000393.4
• NCBI GenBank Nucleotide #: NM_000402.4
• UniProt Accession #: P11413; Q16000; Q16765; Q8IU70; Q8IU88; Q8IUA6; Q96PQ2; D3DWX9
• Molecular Weight: 62,468 Da
• NCBI Official Full Name: glucose-6-phosphate 1-dehydrogenase isoform a
• NCBI Official Synonym Full Names: glucose-6-phosphate dehydrogenase
• NCBI Official Symbol: G6PD
• NCBI Official Synonym Symbols: G6PD1
• NCBI Protein Information: glucose-6-phosphate 1-dehydrogenase
• UniProt Protein Name: Glucose-6-phosphate 1-dehydrogenase
• UniProt Gene Name: G6PD
• UniProt Synonym Gene Names: G6PD
• UniProt Entry Name: G6PD_HUMAN

NCBI Description

This gene encodes glucose-6-phosphate dehydrogenase. This protein is a cytosolic enzyme encoded by a housekeeping X-linked gene whose main function is to produce NADPH, a key electron donor in the defense against oxidizing agents and in reductive biosynthetic reactions. G6PD is remarkable for its genetic diversity. Many variants of G6PD, mostly produced from missense mutations, have been described with wide ranging levels of enzyme activity and associated clinical symptoms. G6PD deficiency may cause neonatal jaundice, acute hemolysis, or severe chronic non-spherocytic hemolytic anemia. Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jul 2008]

Uniprot Description

G6PD: glucose-6-phosphate dehydrogenase. A cytosolic enzyme encoded by a housekeeping X-linked gene whose main function is to produce NADPH, a key electron donor in the defense against oxidizing agents and in reductive biosynthetic reactions. G6PD is remarkable for its genetic diversity. Many variants of G6PD, mostly produced from missense mutations, have been described with wide ranging levels of enzyme activity and associated clinical symptoms. G6PD deficiency may cause neonatal jaundice, acute hemolysis, or severe chronic non-spherocytic hemolytic anemia. Two splice variant isoforms have been described. The long isoform is found in lymphoblasts, granulocytes and sperm.

Protein type: Oxidoreductase; Other Amino Acids Metabolism - glutathione; Cell development/differentiation; Carbohydrate Metabolism - pentose phosphate pathway; EC 1.1.1.49

Chromosomal Location of Human Ortholog: Xq28

Cellular Component: centrosome; internal side of plasma membrane; intracellular membrane-bound organelle; membrane; cytoplasm; microtubule organizing center; cytosol; nucleus

Molecular Function: identical protein binding; protein binding; glucose-6-phosphate dehydrogenase activity; protein homodimerization activity; NADP binding; glucose binding

Biological Process: response to food; NADPH regeneration; pentose-phosphate shunt; erythrocyte maturation; ribose phosphate biosynthetic process; pentose-phosphate shunt, oxidative branch; glucose 6-phosphate metabolic process; cytokine production; glucose metabolic process; pathogenesis; response to organic cyclic substance; NADP metabolic process; cholesterol biosynthetic process; substantia nigra development; response to ethanol; glutathione metabolic process; regulation of neuron apoptosis; carbohydrate metabolic process; lipid metabolic process; pentose biosynthetic process

Disease: Favism, Susceptibility To; Anemia, Nonspherocytic Hemolytic, Due To G6pd Deficiency; Malaria, Susceptibility To