Rabbit anti-Human MAGEA3 Polyclonal Antibody | anti-MAGEA3 antibody

MAGEA3 Antibody

Cat. Num. AAA9411000

• Gene Names: MAGEA3; HIP8; HYPD; CT1.3; MAGE3; MAGEA6
• Reactivity: Human
• Applications: Western Blot, Immunohistochemistry
• Purity: Antigen affinity purification.
• Synonyms: MAGEA3; Polyclonal Antibody; MAGEA3 Antibody; HIP8; HYPD; CT1.3; MAGE3; MAGEA6; anti-MAGEA3 antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Specificity: The antibody detects endogenous levels of total MAGEA3 protein.
• Purity/Purification: Antigen affinity purification.
• Form/Format: Rabbit IgG in pH7.3 PBS, 0.05% NaN3, 50% Glycerol.
• Concentration: 2.9 mg/ml (varies by lot)
• Sequence Length: 314

• Applicable Applications for anti-MAGEA3 antibody: Western Blot (WB), Immunohistochemistry (IHC)
• Application Notes: Western blotting: 1:500-1:2000; Immunohistochemistry: 1:50-1:200
• Immunogen Type: Peptide
• Immunogen Description: Synthetic peptide corresponding to residues near the C terminal of human melanoma antigen family A, 3
• Target Name: MAGEA3
• Preparation and Storage: Store at -20 degree C

Testing Data

(Gel: 10%SDS-PAGE Lysates (from left to right): Human testis tissue, A549 and PC3 cell Amount of lysate: 40ug per lane Primary antibody: 1/400 dilution Secondary antibody dilution: 1/8000 Exposure time: 2 minutes)

Immunohistochemistry (IHC)

(Immunohistochemical analysis of paraffin-embedded Human tonsil tissue using at dilution 1/50.)

Related Product Information for anti-MAGEA3 antibody

This gene is a member of the MAGEA gene family. The members of this family encode proteins with 50 to 80% sequence identity to each other. The promoters and first exons of the MAGEA genes show considerable variability, suggesting that the existence of this gene family enables the same function to be expressed under different transcriptional controls. The MAGEA genes are clustered at chromosomal location Xq28. They have been implicated in some hereditary disorders, such as dyskeratosis congenita.

Product Categories/Family for anti-MAGEA3 antibody

Total protein Ab

NCBI and Uniprot Product Information

• NCBI GI #: 4885467
• NCBI GeneID: 4102
• NCBI Accession #: NP_005353.1
• NCBI GenBank Nucleotide #: NM_005362.3
• UniProt Accession #: P43357; Q6FHI6
• Molecular Weight: 34,747 Da
• NCBI Official Full Name: melanoma-associated antigen 3
• NCBI Official Synonym Full Names: melanoma antigen family A3
• NCBI Official Symbol: MAGEA3
• NCBI Official Synonym Symbols: HIP8; HYPD; CT1.3; MAGE3; MAGEA6
• NCBI Protein Information: melanoma-associated antigen 3
• UniProt Protein Name: Melanoma-associated antigen 3
• Protein Family: Melanoma-associated antigen
• UniProt Gene Name: MAGEA3
• UniProt Synonym Gene Names: MAGE3; CT1.3
• UniProt Entry Name: MAGA3_HUMAN

NCBI Description

This gene is a member of the MAGEA gene family. The members of this family encode proteins with 50 to 80% sequence identity to each other. The promoters and first exons of the MAGEA genes show considerable variability, suggesting that the existence of this gene family enables the same function to be expressed under different transcriptional controls. The MAGEA genes are clustered at chromosomal location Xq28. They have been implicated in some hereditary disorders, such as dyskeratosis congenita. [provided by RefSeq, Jul 2008]

Uniprot Description

MAGEA3: Proposed to enhance ubiquitin ligase activity of RING- type zinc finger-containing E3 ubiquitin-protein ligases. May enhance ubiquitin ligase activity of TRIM28 and stimulate p53/TP53 ubiquitination by TRIM28. Proposed to act through recruitment and/or stabilization of the Ubl-conjugating enzyme (E2) at the E3:substrate complex. May play a role in embryonal development and tumor transformation or aspects of tumor progression. In vitro promotes cell viability in melanoma cell lines. Antigen recognized on a melanoma by autologous cytolytic T-lymphocytes.

Protein type: Cancer Testis Antigen (CTA)

Chromosomal Location of Human Ortholog: Xq28

Molecular Function: protein binding