Rabbit MGMT Polyclonal Antibody | anti-MGMT antibody

MGMT antibody

Cat. Num. AAA9409314

• Reactivity: Human, Mouse, Rat
• Applications: Western Blot, Immunofluorescence
• Purity: Antibodies were purified by affinity purification using immunogen.
• Synonyms: MGMT; Polyclonal Antibody; MGMT antibody; anti-MGMT antibody

• Host: Rabbit
• Reactivity: Human, Mouse, Rat
• Clonality: Polyclonal
• Specificity: The antibody detects endogenous level of total MGMT antibody.
• Purity/Purification: Antibodies were purified by affinity purification using immunogen.
• Form/Format: Supplied at 1.0mg/mL in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
• Concentration: 1.0 mg/ml (varies by lot)
• Sequence Length: 207

• Applicable Applications for anti-MGMT antibody: Western Blot (WB), Immunofluorescence (IF)
• Application Notes: Western blotting: 1:500 - 1:2000; Immunofluorescence: 1:50 - 1:200
• Immunogen Type: Recombinant Protein
• Immunogen Description: Recombinant protein of human MGMT.
• Target Name: MGMT
• Acession Number: P16455NCBI
• Gene ID: 4255
• Preparation and Storage: Store at -20 degree C

Western Blot (WB)

(Western blot analysis of Hela cell lysate using MGMT antibody.)

Immunofluorescence (IF)

(Immunofluorescence analysis of U20S cell using MGMT antibody. Blue: DAPI for nuclear staining.)

Related Product Information for anti-MGMT antibody

MGMT (O-6-methylguanine-DNA methyltransferase) is a DNA repair enzyme that participates in a suicide reaction that specifically removes methyl or alkyl groups from the O(6) position of guanine, restoring guanine to its normal form without causing DNA breaks (1). MGMT protects cells from alkylating toxins, and is an important factor in drug resistance to alkylating therapeutic agents (2,3). It is ubiquitously expressed in normal human tissues (4) and is overexpressed in many types of human tumors, but epigenetically silenced in other tumors. MGMT silencing is a marker associated with poor prognosis, but is a good predictive marker for response to alkylating agent chemotherapy (5).

Product Categories/Family for anti-MGMT antibody

Total protein Ab

NCBI and Uniprot Product Information

• NCBI GI #: 530394832
• NCBI GeneID: 4255
• NCBI Accession #: XP_005252739.1
• NCBI GenBank Nucleotide #: XM_005252682.2
• UniProt Accession #: P16455; Q5VY78
• Molecular Weight: 22kd
• NCBI Official Full Name: methylated-DNA--protein-cysteine methyltransferase isoform X2
• NCBI Official Synonym Full Names: O-6-methylguanine-DNA methyltransferase
• NCBI Official Symbol: MGMT
• NCBI Protein Information: methylated-DNA--protein-cysteine methyltransferase
• UniProt Protein Name: Methylated-DNA--protein-cysteine methyltransferase
• Protein Family: Probable methylated-DNA--protein-cysteine methyltransferase
• UniProt Gene Name: MGMT
• UniProt Synonym Gene Names: MGMT
• UniProt Entry Name: MGMT_HUMAN

Uniprot Description

MGMT: Involved in the cellular defense against the biological effects of O6-methylguanine (O6-MeG) in DNA. Repairs alkylated guanine in DNA by stoichiometrically transferring the alkyl group at the O-6 position to a cysteine residue in the enzyme. This is a suicide reaction: the enzyme is irreversibly inactivated. Belongs to the MGMT family.

Protein type: Nuclear receptor co-regulator; Methyltransferase; DNA repair, damage; EC 2.1.1.63; Apoptosis

Chromosomal Location of Human Ortholog: 10q26

Cellular Component: nucleoplasm; membrane; nucleus

Molecular Function: methyltransferase activity; methylated-DNA-[protein]-cysteine S-methyltransferase activity; DNA binding; damaged DNA binding; calcium ion binding; DNA-methyltransferase activity

Biological Process: response to drug; response to ethanol; positive regulation of DNA repair; DNA dealkylation; response to toxin; response to folic acid; DNA methylation; regulation of caspase activity; DNA repair; DNA ligation