Rabbit anti-Human, Mouse IRF-3 Polyclonal Antibody | anti-IRF-3 antibody

IRF-3 (Phospho-Ser385) Antibody

Cat. Num. AAA9408677

• Reactivity: Human, Mouse
• Applications: Western Blot
• Purity: Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy usi
• Synonyms: IRF-3; Polyclonal Antibody; IRF-3 (Phospho-Ser385) Antibody; Interferon regulatory factor 3; anti-IRF-3 antibody

• Host: Rabbit
• Reactivity: Human, Mouse
• Clonality: Polyclonal
• Specificity: The antibody detects endogenous level of IRF-3 only when phosphorylated at Serine 385.
• Purity/Purification: Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy usi
• Form/Format: Supplied at 1.0mg/mL in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
• Concentration: 1.0 mg/ml (varies by lot)
• Sequence Length: 452

• Applicable Applications for anti-IRF-3 antibody: Western Blot (WB)
• Application Notes: Western blotting: 1:500~1:1000
• Immunogen Type: Peptide-KLH
• Immunogen Description: Peptide sequence around phosphorylation site of Serine 385
• Modification: Phospho-Ser385
• Target Name: IRF-3
• Preparation and Storage: Store at -20 degree C/1 year

Western Blot (WB)

(Western blot analysis of extracts from Mouse heart tissue using IRF-3 (Phospho-Ser385) Antibody.The lane on the right is treated with the antigen-specific peptide.)

Related Product Information for anti-IRF-3 antibody

Key transcriptional regulator of type I interferon (IFN)-dependent immune responses and plays a critical role in the innate immune response against DNA and RNA viruses. Regulates the transcription of type I IFN genes (IFN-alpha and IFN-beta) and IFN-stimulated genes (ISG) by binding to an interferon-stimulated response element (ISRE) in their promoters. Acts as a more potent activator of the IFN-beta (IFNB) gene than the IFN-alpha (IFNA) gene and plays a critical role in both the early and late phases of the IFNA/B gene induction. Found in an inactive form in the cytoplasm of uninfected cells and following viral infection, double-stranded RNA (dsRNA), or toll-like receptor (TLR) signaling, becomes phosphorylated by IKBKE and TBK1 kinases. This induces a conformational change, leading to its dimerization and nuclear localization and association with CREB binding protein (CREBBP) to form dsRNA-activated factor 1 (DRAF1), a complex which activates the transcription of the type I IFN and ISG genes. Can activate distinct gene expression programs in macrophages and can induce significant apoptosis in primary macrophages.

Product Categories/Family for anti-IRF-3 antibody

Phospho-specific Ab

NCBI and Uniprot Product Information

• NCBI GI #: 308199446
• NCBI GeneID: 3661
• NCBI Accession #: NP_001184051.1
• NCBI GenBank Nucleotide #: NM_001197122.1
• UniProt Accession #: Q14653; Q5FBY1; Q5FBY2; Q5FBY4; Q7Z5G6; A8K7L2; B2RAZ3
• Molecular Weight: 12,330 Da
• NCBI Official Full Name: interferon regulatory factor 3 isoform 2
• NCBI Official Synonym Full Names: interferon regulatory factor 3
• NCBI Official Symbol: IRF3
• NCBI Protein Information: interferon regulatory factor 3
• UniProt Protein Name: Interferon regulatory factor 3
• UniProt Gene Name: IRF3
• UniProt Synonym Gene Names: IRF-3
• UniProt Entry Name: IRF3_HUMAN

NCBI Description

This gene encodes a member of the interferon regulatory transcription factor (IRF) family. The encoded protein is found in an inactive cytoplasmic form that upon serine/threonine phosphorylation forms a complex with CREBBP. This complex translocates to the nucleus and activates the transcription of interferons alpha and beta, as well as other interferon-induced genes. Alternatively spliced transcript variants encoding multiple isoforms have been observed for this gene. [provided by RefSeq, Nov 2011]

Uniprot Description

IRF3: interferon regulatory factor 3, a member of the interferon regulatory transcription factor (IRF) family. IRF3 is found in an inactive cytoplasmic form that upon serine/threonine phosphorylation forms a complex with CREBBP. This complex translocates to the nucleus and activates the transcription of interferons alpha and beta, as well as other interferon-induced genes.

Protein type: DNA-binding; Transcription factor

Chromosomal Location of Human Ortholog: 19q13.3-q13.4

Cellular Component: nucleoplasm; cytoplasm; cytosol; nucleus

Molecular Function: identical protein binding; protein binding; protein homodimerization activity; DNA binding; transcription cofactor activity; transcription factor activity

Biological Process: transcription from RNA polymerase II promoter; negative regulation of interferon-beta biosynthetic process; positive regulation of I-kappaB kinase/NF-kappaB cascade; viral reproduction; apoptosis; MyD88-independent toll-like receptor signaling pathway; cytokine and chemokine mediated signaling pathway; negative regulation of transcription from RNA polymerase II promoter; negative regulation of defense response to virus by host; toll-like receptor 3 signaling pathway; response to exogenous dsRNA; positive regulation of interferon-beta production; positive regulation of interferon type I production; lipopolysaccharide-mediated signaling pathway; toll-like receptor signaling pathway; innate immune response; interferon type I biosynthetic process; defense response to virus; response to DNA damage stimulus; negative regulation of interferon type I production; toll-like receptor 4 signaling pathway; positive regulation of interferon-alpha production; positive regulation of cytokine secretion

Disease: Herpes Simplex Encephalitis, Susceptibility To, 7