Mouse IRF3 Monoclonal Antibody | anti-IRF3 antibody

IRF3 Antibody

Cat. Num. AAA9215402

• Reactivity: Human, African Green Monkey
• Applications: Western Blot
• Synonyms: IRF3; Monoclonal Antibody; IRF3 Antibody; Purified Mouse Monoclonal Antibody (Mab); Interferon regulatory factor 3; IRF-3; anti-IRF3 antibody

• Host: Mouse
• Reactivity: Human, African Green Monkey
• Clonality: Monoclonal
• Isotype: IgG1, k
• Clone Number: 1522CT766.58.24
• Specificity: This IRF3 antibody is generated from a mouse immunized with a recombinant protein.
• Form/Format: Purified monoclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein G column, followed by dialysis against PBS.
• Sequence Length: 452

• Applicable Applications for anti-IRF3 antibody: Western Blot (WB)
• Application Notes: WB ~~ 1:2000
• Antigen Source: Human
• Preparation and Storage: Maintain refrigerated at 2-8 degree C for up to 6 months. For long term storage store at -20 degree C in small aliquots to prevent freeze-thaw cycles.

Western Blot (WB)

(All lanes : Anti-IRF3 Antibody at 1:2000 dilutionLane 1: COS-7 whole cell lysateLane 2: Daudi whole cell lysateLane 3: HepG2 whole cell lysateLane 4: HT-29 whole cell lysateLane 5: Jurkat whole cell lysateLane 6: MCF-7 whole cell lysate Lysates/proteins at 20 mug per lane.SecondaryGoat Anti-mouse IgG, (H+L), Peroxidase conjugated at 1/10000 dilution.Predicted band size : 47 kDaBlocking/Dilution buffer: 5% NFDM/TBST.)

Related Product Information for anti-IRF3 antibody

Key transcriptional regulator of type I interferon (IFN)-dependent immune responses which plays a critical role in the innate immune response against DNA and RNA viruses. Regulates the transcription of type I IFN genes (IFN-alpha and IFN-beta) and IFN-stimulated genes (ISG) by binding to an interferon-stimulated response element (ISRE) in their promoters. Acts as a more potent activator of the IFN-beta (IFNB) gene than the IFN-alpha (IFNA) gene and plays a critical role in both the early and late phases of the IFNA/B gene induction. Found in an inactive form in the cytoplasm of uninfected cells and following viral infection, double-stranded RNA (dsRNA), or toll-like receptor (TLR) signaling, is phosphorylated by IKBKE and TBK1 kinases. This induces a conformational change, leading to its dimerization and nuclear localization and association with CREB binding protein (CREBBP) to form dsRNA-activated factor 1 (DRAF1), a complex which activates the transcription of the type I IFN and ISG genes. Can activate distinct gene expression programs in macrophages and can induce significant apoptosis in primary macrophages.

Product Categories/Family for anti-IRF3 antibody

Crown Antibodies; Immunology

References

Au W.W.-C.,et al.Proc. Natl. Acad. Sci. U.S.A. 92:11657-11661(1995). Tabata Y.,et al.Submitted (FEB-2003) to the EMBL/GenBank/DDBJ databases. Ota T.,et al.Nat. Genet. 36:40-45(2004). Grimwood J.,et al.Nature 428:529-535(2004). Mural R.J.,et al.Submitted (JUL-2005) to the EMBL/GenBank/DDBJ databases.

NCBI and Uniprot Product Information

• NCBI GI #: 308199446
• NCBI GeneID: 3661
• NCBI Accession #: NP_001184051.1
• NCBI GenBank Nucleotide #: NM_001197122.1
• UniProt Accession #: Q14653; Q5FBY1; Q5FBY2; Q5FBY4; Q7Z5G6; A8K7L2; B2RAZ3
• Molecular Weight: 47219
• NCBI Official Full Name: interferon regulatory factor 3 isoform 2
• NCBI Official Synonym Full Names: interferon regulatory factor 3
• NCBI Official Symbol: IRF3
• NCBI Protein Information: interferon regulatory factor 3
• UniProt Protein Name: Interferon regulatory factor 3
• Protein Family: Interferon regulatory factor
• UniProt Gene Name: IRF3
• UniProt Synonym Gene Names: IRF-3
• UniProt Entry Name: IRF3_HUMAN

NCBI Description

This gene encodes a member of the interferon regulatory transcription factor (IRF) family. The encoded protein is found in an inactive cytoplasmic form that upon serine/threonine phosphorylation forms a complex with CREBBP. This complex translocates to the nucleus and activates the transcription of interferons alpha and beta, as well as other interferon-induced genes. Alternatively spliced transcript variants encoding multiple isoforms have been observed for this gene. [provided by RefSeq, Nov 2011]

Uniprot Description

IRF3: interferon regulatory factor 3, a member of the interferon regulatory transcription factor (IRF) family. IRF3 is found in an inactive cytoplasmic form that upon serine/threonine phosphorylation forms a complex with CREBBP. This complex translocates to the nucleus and activates the transcription of interferons alpha and beta, as well as other interferon-induced genes.

Protein type: Transcription factor; DNA-binding

Chromosomal Location of Human Ortholog: 19q13.3-q13.4

Cellular Component: nucleoplasm; cytoplasm; cytosol; nucleus

Molecular Function: identical protein binding; protein binding; protein homodimerization activity; DNA binding; transcription cofactor activity; transcription factor activity

Biological Process: transcription from RNA polymerase II promoter; positive regulation of I-kappaB kinase/NF-kappaB cascade; viral reproduction; apoptosis; MyD88-independent toll-like receptor signaling pathway; cytokine and chemokine mediated signaling pathway; toll-like receptor 3 signaling pathway; response to exogenous dsRNA; regulation of transcription, DNA-dependent; lipopolysaccharide-mediated signaling pathway; toll-like receptor signaling pathway; positive regulation of interferon type I production; positive regulation of interferon-beta production; innate immune response; interferon type I biosynthetic process; response to DNA damage stimulus; negative regulation of interferon type I production; toll-like receptor 4 signaling pathway; defense response to virus; positive regulation of interferon-alpha production; positive regulation of cytokine secretion

Disease: Herpes Simplex Encephalitis, Susceptibility To, 7