Rabbit anti-Human PTPN6 Polyclonal Antibody | anti-PTPN6 antibody

PTPN6 Antibody

Cat. Num. AAA9408121

• Gene Names: PTPN6; HCP; HCPH; SHP1; SHP-1; HPTP1C; PTP-1C; SHP-1L; SH-PTP1
• Reactivity: Human
• Applications: ELISA, Western Blot, Immunohistochemistry
• Synonyms: PTPN6; Polyclonal Antibody; PTPN6 Antibody; Protein tyrosine phosphatase; non-receptor type 6; HCP; HCPH; SHP1; SHP-1; HPTP1C; PTP-1C; SHP-1L; SH-PTP1; anti-PTPN6 antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Specificity: The antibody detects endogenous level of total PTPN6 protein.
• Form/Format: Supplied at 1mg/mL in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.3, 0.05% sodium azide and 50% glycerol.
• Sequence Length: 2220

• Applicable Applications for anti-PTPN6 antibody: ELISA (EIA), Western Blot (WB), Immunohistochemistry (IHC)
• Application Notes: ELISA: 1:1000-1:2000; Western blotting: 1:200-1:1000; Immunohistochemistry: 1:50-1:200
• Immunogen Type: Recombinant protein
• Immunogen Description: Fusion protein corresponding to a region derived from 244-515 amino acids of human protein tyrosine phosphatase, non-receptor type 6
• Target Name: PTPN6
• Preparation and Storage: Store at -20 degree C/1 year

Testing Data

(Gel: 10%SDS-PAGELane1: A375 cell lysateLane2: A431 cell lysateLysates: 40 ug per lanePrimary antibody: 1/500 dilutionSecondary antibody: Goat anti Rabbit IgG - H&L (HRP) at 1/10000 dilutionExposure time: 90 seconds)

Immunohistochemistry (IHC)

(The image on the left is immunohistochemistry of paraffin-embedded human liver cancer tissue using 31122 (PTPN6 Antibody) at dilution 1/35, on the right is treated with the fusion protein.)

Related Product Information for anti-PTPN6 antibody

The protein encoded by this gene is a member of the protein tyrosine phosphatase (PTP) family. PTPs are known to be signaling molecules that regulate a variety of cellular processes including cell growth, differentiation, mitotic cycle, and oncogenic transformation. N-terminal part of this PTP contains two tandem Src homolog (SH2) domains, which act as protein phospho-tyrosine binding domains, and mediate the interaction of this PTP with its substrates. This PTP is expressed primarily in hematopoietic cells, and functions as an important regulator of multiple signaling pathways in hematopoietic cells. This PTP has been shown to interact with, and dephosphorylate a wide spectrum of phospho-proteins involved in hematopoietic cell signaling. Multiple alternatively spliced variants of this gene, which encode distinct isoforms, have been reported.

Product Categories/Family for anti-PTPN6 antibody

Total protein Ab

NCBI and Uniprot Product Information

• NCBI GI #: 33876814
• NCBI GeneID: 5777
• Molecular Weight: 70,131 Da
• NCBI Official Full Name: Homo sapiens protein tyrosine phosphatase, non-receptor type 6, mRNA
• NCBI Official Synonym Full Names: protein tyrosine phosphatase, non-receptor type 6
• NCBI Official Symbol: PTPN6
• NCBI Official Synonym Symbols: HCP; HCPH; SHP1; SHP-1; HPTP1C; PTP-1C; SHP-1L; SH-PTP1
• NCBI Protein Information: tyrosine-protein phosphatase non-receptor type 6
• UniProt Protein Name: Tyrosine-protein phosphatase non-receptor type 6
• Protein Family: Tyrosine-protein phosphatase non-receptor
• UniProt Gene Name: PTPN6
• UniProt Synonym Gene Names: HCP; PTP1C; PTP-1C
• UniProt Entry Name: PTN6_HUMAN

NCBI Description

The protein encoded by this gene is a member of the protein tyrosine phosphatase (PTP) family. PTPs are known to be signaling molecules that regulate a variety of cellular processes including cell growth, differentiation, mitotic cycle, and oncogenic transformation. N-terminal part of this PTP contains two tandem Src homolog (SH2) domains, which act as protein phospho-tyrosine binding domains, and mediate the interaction of this PTP with its substrates. This PTP is expressed primarily in hematopoietic cells, and functions as an important regulator of multiple signaling pathways in hematopoietic cells. This PTP has been shown to interact with, and dephosphorylate a wide spectrum of phospho-proteins involved in hematopoietic cell signaling. Multiple alternatively spliced variants of this gene, which encode distinct isoforms, have been reported. [provided by RefSeq, Jul 2008]

Uniprot Description

SHP-1: an SH2-containing ubiquitously expressed tyrosine-specific protein phosphatase. Plays a key role in hematopoiesis. Directly links growth factor receptors and other signaling proteins through protein-tyrosine phosphorylation. The SH2 regions may interact with other cellular components to modulate its own phosphatase activity against interacting substrates. Three alternatively spliced isoforms have been described.

Protein type: EC 3.1.3.48; Motility/polarity/chemotaxis; Protein phosphatase, tyrosine (non-receptor)

Chromosomal Location of Human Ortholog: 12p13

Cellular Component: alpha-beta T cell receptor complex; cytoplasm; cytosol; intercellular junction; membrane; nucleolus; nucleus

Molecular Function: phosphotyrosine binding; protein binding; protein kinase binding; protein tyrosine phosphatase activity; SH2 domain binding; SH3 domain binding; transmembrane receptor protein tyrosine phosphatase activity

Biological Process: abortive mitotic cell cycle; apoptosis; B cell receptor signaling pathway; blood coagulation; cell differentiation; cell proliferation; cytokine and chemokine mediated signaling pathway; G-protein coupled receptor protein signaling pathway; leukocyte migration; natural killer cell mediated cytotoxicity; negative regulation of B cell receptor signaling pathway; negative regulation of cell proliferation; negative regulation of humoral immune response mediated by circulating immunoglobulin; negative regulation of MAP kinase activity; negative regulation of peptidyl-tyrosine phosphorylation; negative regulation of T cell proliferation; negative regulation of T cell receptor signaling pathway; peptidyl-tyrosine phosphorylation; platelet activation; platelet formation; positive regulation of cell adhesion mediated by integrin; positive regulation of cell proliferation; positive regulation of phosphoinositide 3-kinase cascade; protein amino acid dephosphorylation; regulation of B cell differentiation; regulation of release of sequestered calcium ion into cytosol; T cell costimulation