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Western Blot (WB) (Western blot analysis of extracts from HuvEC cells, using DGKB antibody.)

Rabbit anti-Human DGKB Polyclonal Antibody | anti-DGKB antibody

DGKB Antibody

Gene Names
DGKB; DGK; DAGK2; DGK-BETA
Reactivity
Human
Applications
Western Blot
Purity
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Synonyms
DGKB; Polyclonal Antibody; DGKB Antibody; 90 kDa diacylglycerol kinase; DAG kinase beta; DAGK2; DGK-beta; anti-DGKB antibody
Ordering
For Research Use Only!
Host
Rabbit
Reactivity
Human
Clonality
Polyclonal
Specificity
The antibody detects endogenous levels of total DGKB protein.
Purity/Purification
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Form/Format
Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Concentration
1.0 mg/ml (varies by lot)
Sequence Length
804
Applicable Applications for anti-DGKB antibody
Western Blot (WB)
Application Notes
Western blotting: 1:500~1:3000
Immunogen Type
Peptide
Immunogen Description
Synthesized peptide derived from internal of human DGKB.
Target Name
DGKB
Preparation and Storage
Store at -20 degree C

Western Blot (WB)

(Western blot analysis of extracts from HuvEC cells, using DGKB antibody.)

Western Blot (WB) (Western blot analysis of extracts from HuvEC cells, using DGKB antibody.)
Related Product Information for anti-DGKB antibody
Exhibits high phosphorylation activity for long-chain diacylglycerols By similarity.Wilson R.K., Nature 424:157-164(2003).Venter J.C., Submitted (JUL-2005).Ohara O., DNA Res. 5:277-286(1998).
Product Categories/Family for anti-DGKB antibody

NCBI and Uniprot Product Information

NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
87,051 Da
NCBI Official Full Name
diacylglycerol kinase beta isoform 1
NCBI Official Synonym Full Names
diacylglycerol kinase, beta 90kDa
NCBI Official Symbol
DGKB
NCBI Official Synonym Symbols
DGK; DAGK2; DGK-BETA
NCBI Protein Information
diacylglycerol kinase beta
UniProt Protein Name
Diacylglycerol kinase beta
Protein Family
UniProt Gene Name
DGKB
UniProt Synonym Gene Names
DAGK2; KIAA0718; DAG kinase beta; DGK-beta
UniProt Entry Name
DGKB_HUMAN

NCBI Description

Diacylglycerol kinases (DGKs) are regulators of the intracellular concentration of the second messenger diacylglycerol (DAG) and thus play a key role in cellular processes. Nine mammalian isotypes have been identified, which are encoded by separate genes. Mammalian DGK isozymes contain a conserved catalytic (kinase) domain and a cysteine-rich domain (CRD). The protein encoded by this gene is a diacylglycerol kinase, beta isotype. Two alternatively spliced transcript variants have been found for this gene. [provided by RefSeq, Jul 2008]

Uniprot Description

DGKB: is a cytoplasmic enzyme that phosphorylates 1,2-diacylglycerol. A regulator of the intracellular concentration of the second messenger diacylglycerol (DAG) and thus play a key role in cellular processes. Nine mammalian isotypes have been identified, which are encoded by separate genes. Mammalian DGK isozymes contain a conserved catalytic (kinase) domain and a cysteine-rich domain (CRD).

Protein type: Lipid Metabolism - glycerolipid; EC 2.7.1.107; Lipid Metabolism - glycerophospholipid; Kinase, lipid; Motility/polarity/chemotaxis

Chromosomal Location of Human Ortholog: 7p21.2

Cellular Component: cytoplasm; plasma membrane

Molecular Function: calcium ion binding; diacylglycerol kinase activity; ATP binding; NAD+ kinase activity

Biological Process: platelet activation; protein kinase C activation; blood coagulation; phosphorylation

Research Articles on DGKB

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Product Notes

The DGKB dgkb (Catalog #AAA9407788) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The DGKB Antibody reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's DGKB can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB). Western blotting: 1:500~1:3000. Researchers should empirically determine the suitability of the DGKB dgkb for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "DGKB, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.

Precautions

All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.

Disclaimer

Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.

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