Rabbit Gab2 Polyclonal Antibody | anti-Gab2 antibody

Gab2 (Phospho-Tyr643) Antibody

Cat. Num. AAA9407337

• Reactivity: Human, Mouse, Rat
• Applications: Western Blot, Immunohistochemistry
• Purity: Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy usi
• Synonyms: Gab2; Polyclonal Antibody; Gab2 (Phospho-Tyr643) Antibody; pp100; GABJ; KIAA0571; anti-Gab2 antibody

• Host: Rabbit
• Reactivity: Human, Mouse, Rat
• Clonality: Polyclonal
• Specificity: The antibody detects endogenous levels of Gab2 only when phosphorylated at tyrosine 643.
• Purity/Purification: Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy usi
• Form/Format: Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
• Concentration: 1.0 mg/ml (varies by lot)
• Sequence Length: 638

• Applicable Applications for anti-Gab2 antibody: Western Blot (WB), Immunohistochemistry (IHC)
• Application Notes: Western blotting: 1:500~1:1000; Immunohistochemistry: 1:50~1:100
• Immunogen Type: Peptide-KLH
• Immunogen Description: Peptide sequence around phosphorylation site of tyrosine 643(V-D-Y(p)-V-Q) derived from Human Gab2.
• Modification: Phospho-Tyr643
• Target Name: Gab2
• Preparation and Storage: Store at -20 degree C/1 year

Western Blot (WB)

(Western blot analysis of extracts from 3T3 cells (Lane 2), using Gab2 (Phospho-Tyr643) Antibody. The lane on the left is treated with antigen-specific peptide.)

Immunohistochemistry (IHC)

(Immunohistochemical analysis of paraffin-embedded human brain tissue using Gab2 (Phospho-Tyr643) antibody (left)or the same antibody preincubated with blocking peptide (right).)

Related Product Information for anti-Gab2 antibody

This gene is a member of the GRB2-associated binding protein (GAB) gene family and is similar to the GAB1 gene. These proteins contain pleckstrin homology (PH) domain, and bind SHP2 tyrosine phosphatase and GRB2 adapter protein. They act as adapters for transmitting various signals in response to stimuli through cytokine and growth factor receptors, and T- and B-cell antigen receptors. The protein encoded by this gene is the principal activator of phosphatidylinositol-3 kinase in response to activation of the high affinity IgE receptor. Two alternatively spliced transcripts encoding different isoforms have been described for this gene.Nishida K., Blood 93:1809-1816(1999).Nagase T., DNA Res. 5:31-39(1998).Salomon A.R., Proc. Natl. Acad. Sci. U.S.A. 100:443-448(2003).

Product Categories/Family for anti-Gab2 antibody

Phospho-specific Ab

NCBI and Uniprot Product Information

• NCBI GI #: 6912460
• NCBI GeneID: 9846
• NCBI Accession #: NP_036428.1
• NCBI GenBank Nucleotide #: NM_012296.3
• UniProt Accession #: Q9UQC2; O60317; A2RRM2; A6NEW9; A7MD36
• Molecular Weight: 69,968 Da
• NCBI Official Full Name: GRB2-associated-binding protein 2 isoform b
• NCBI Official Synonym Full Names: GRB2-associated binding protein 2
• NCBI Official Symbol: GAB2
• NCBI Protein Information: GRB2-associated-binding protein 2
• UniProt Protein Name: GRB2-associated-binding protein 2
• Protein Family: GRB2-associated-binding protein
• UniProt Gene Name: GAB2
• UniProt Synonym Gene Names: KIAA0571
• UniProt Entry Name: GAB2_HUMAN

NCBI Description

This gene is a member of the GRB2-associated binding protein (GAB) gene family. These proteins contain pleckstrin homology (PH) domain, and bind SHP2 tyrosine phosphatase and GRB2 adapter protein. They act as adapters for transmitting various signals in response to stimuli through cytokine and growth factor receptors, and T- and B-cell antigen receptors. The protein encoded by this gene is the principal activator of phosphatidylinositol-3 kinase in response to activation of the high affinity IgE receptor. Two alternatively spliced transcripts encoding different isoforms have been described for this gene. [provided by RefSeq, Nov 2009]

Uniprot Description

GAB2: an adaptor protein of the GRB2-associated binding protein family. Plays important roles in regulating the growth, differentiation, and function of many hematopoietic cell types.

Protein type: Adaptor/scaffold

Chromosomal Location of Human Ortholog: 11q14.1

Cellular Component: cytoplasm; plasma membrane; cytosol

Molecular Function: protein binding; phosphatidylinositol-3,4,5-triphosphate binding; phosphatidylinositol-3,4-bisphosphate binding; transmembrane receptor protein tyrosine kinase adaptor protein activity

Biological Process: integrin-mediated signaling pathway; cell migration; phosphoinositide-mediated signaling; positive regulation of cell proliferation; innate immune response; positive regulation of mast cell degranulation; osteoclast differentiation; transmembrane receptor protein tyrosine kinase signaling pathway