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Western Blot (WB) (Western blot analysis of extracts from COLO cells, using ELOA3 antibody.)

Rabbit anti-Human ELOA3 Polyclonal Antibody | anti-ELOA3 antibody

ELOA3 Antibody

Gene Names
TCEB3C; HsT829; TCEB3L2
Reactivity
Human
Applications
Western Blot
Purity
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Synonyms
ELOA3; Polyclonal Antibody; ELOA3 Antibody; RNA polymerase II transcription factor SIII subunit A3; Elongin-A3; EloA3; Transcription elongation factor B polypeptide 3C; TCEB3C; anti-ELOA3 antibody
Ordering
For Research Use Only!
Host
Rabbit
Reactivity
Human
Clonality
Polyclonal
Specificity
The antibody detects endogenous levels of total ELOA3 protein.
Purity/Purification
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Form/Format
Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Concentration
1.0 mg/ml (varies by lot)
Sequence Length
546
Applicable Applications for anti-ELOA3 antibody
Western Blot (WB)
Application Notes
Western blotting: 1:500~1:3000
Immunogen Type
Peptide
Immunogen Description
Synthesized peptide derived from internal of human ELOA3.
Target Name
ELOA3
Preparation and Storage
Store at -20 degree C

Western Blot (WB)

(Western blot analysis of extracts from COLO cells, using ELOA3 antibody.)

Western Blot (WB) (Western blot analysis of extracts from COLO cells, using ELOA3 antibody.)
Related Product Information for anti-ELOA3 antibody
SIII, also known as elongin, is a general transcription elongation factor that increases the RNA polymerase II transcription elongation past template-encoded arresting sites. Subunit A3 is transcriptionally active but its transcription activity is not enhanced by binding to the dimeric complex of the SIII regulatory subunits B and C (elongin BC complex).Yamazaki K., J. Biol. Chem. 277:26444-26451(2002).
Product Categories/Family for anti-ELOA3 antibody

NCBI and Uniprot Product Information

NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
Molecular Weight
59,744 Da
NCBI Official Full Name
RNA polymerase II transcription factor SIII subunit A3
NCBI Official Synonym Full Names
transcription elongation factor B polypeptide 3C (elongin A3)
NCBI Official Symbol
TCEB3C
NCBI Official Synonym Symbols
HsT829; TCEB3L2
NCBI Protein Information
RNA polymerase II transcription factor SIII subunit A3
UniProt Protein Name
RNA polymerase II transcription factor SIII subunit A3
UniProt Gene Name
TCEB3C
UniProt Synonym Gene Names
TCEB3L2; EloA3
UniProt Entry Name
ELOA3_HUMAN

NCBI Description

The SIII (or elongin) transcription elongation factor complex stimulates the rate of transcription elongation by RNA polymerase II by suppressing the transient pausing of the polymerase at many sites along the DNA template. This complex is a heterotrimer, composed of the transcriptionally active subunit A, A2 or A3 (or elongin A, A2 or A3) and two regulatory subunits, B and C (or elongin B and C). This gene encodes subunit A3. A3 and A are ubiquitously expressed, whereas A2 is specifically expressed in the testis. [provided by RefSeq, Mar 2010]

Uniprot Description

TCEB3C: SIII, also known as elongin, is a general transcription elongation factor that increases the RNA polymerase II transcription elongation past template-encoded arresting sites. Subunit A3 is transcriptionally active but its transcription activity is not enhanced by binding to the dimeric complex of the SIII regulatory subunits B and C (elongin BC complex).

Chromosomal Location of Human Ortholog: 18q21.1

Cellular Component: integral to membrane; nucleus

Molecular Function: DNA binding

Biological Process: transcription, DNA-dependent; regulation of transcription, DNA-dependent

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Product Notes

The ELOA3 tceb3c (Catalog #AAA9406563) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The ELOA3 Antibody reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's ELOA3 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB). Western blotting: 1:500~1:3000. Researchers should empirically determine the suitability of the ELOA3 tceb3c for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "ELOA3, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.

Precautions

All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.

Disclaimer

Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.

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