Rabbit MIF Polyclonal Antibody | anti-MIF antibody

MIF Antibody

Cat. Num. AAA9404804

• Gene Names: MIF; GIF; GLIF; MMIF
• Reactivity: Human, Mouse, Rat
• Applications: Western Blot, Immunohistochemistry
• Purity: Antibodies were purified by affinity purification using immunogen.
• Synonyms: MIF; Polyclonal Antibody; MIF Antibody; GIF; GLIF; MMIF; anti-MIF antibody

• Host: Rabbit
• Reactivity: Human, Mouse, Rat
• Clonality: Polyclonal
• Specificity: The antibody detects endogenous level of total MIF protein.
• Purity/Purification: Antibodies were purified by affinity purification using immunogen.
• Form/Format: Supplied at 1.0mg/mL in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
• Concentration: 1.0 mg/ml (varies by lot)
• Sequence Length: 115

• Applicable Applications for anti-MIF antibody: Western Blot (WB), Immunohistochemistry (IHC)
• Application Notes: Western blotting: 1:500 - 1:2000; Immunohistochemistry: 1:50 - 1:100
• Immunogen Type: Recombinant Protein
• Immunogen Description: Recombinant protein of human MIF.
• Target Name: MIF
• Preparation and Storage: Store at -20 degree C

Western Blot (WB)

(Western blot analysis of extracts of Jurkat cell lines, using MIF antibody.)

Immunohistochemistry (IHC)

(Immunohistochemical analysis of paraffin-embedded mouse lung using MIF antibody at dilution of 1:100 (400x lens).)

Related Product Information for anti-MIF antibody

Macrophage migration inhibitory factor, known as MIF or glycosylation-inhibiting factor, is a secreted, homotrimeric, pro-inflammatory cytokine that modulates macrophage and T cell function and is an important regulator of host response to infection. MIF is expressed at sites of inflammation, which suggests that it plays a role in regulating macrophage function in host defense. MIF is produced by the pituitary gland and is found in monocytes, macrophages, differentiating immunological cells in the eye lens and brain, and fibroblasts. Elevated levels of MIF protein are detected in the plasma of patients with severe sepsis or septic shock, a condition where MIF influences endotoxic shock by enhancing the production of other inflammatory cytokines including tumor necrosis factor (TNF ), interleukin-1 (IL-1) and interferon- (IFN- ). MIF promotes the systemic inflammatory response by counter-regulating glucocorticoid-mediated inhibition of immune-cell activation and proinflammatory cytokine production. MIF may mediate tissue destruction through the induction of proteinases.

Product Categories/Family for anti-MIF antibody

Total protein Ab

NCBI and Uniprot Product Information

• NCBI GI #: 4505185
• NCBI GeneID: 4282
• NCBI Accession #: NP_002406.1
• NCBI GenBank Nucleotide #: NM_002415.1
• UniProt Accession #: P14174; Q2V4Y5; Q6FHV0; A5Z1R8; B2R4S3
• Molecular Weight: 12,476 Da
• NCBI Official Full Name: macrophage migration inhibitory factor
• NCBI Official Synonym Full Names: macrophage migration inhibitory factor (glycosylation-inhibiting factor)
• NCBI Official Symbol: MIF
• NCBI Official Synonym Symbols: GIF; GLIF; MMIF
• NCBI Protein Information: macrophage migration inhibitory factor
• UniProt Protein Name: Macrophage migration inhibitory factor
• Protein Family: MIF-like protein
• UniProt Gene Name: MIF
• UniProt Synonym Gene Names: GLIF; MMIF; MIF; GIF
• UniProt Entry Name: MIF_HUMAN

NCBI Description

This gene encodes a lymphokine involved in cell-mediated immunity, immunoregulation, and inflammation. It plays a role in the regulation of macrophage function in host defense through the suppression of anti-inflammatory effects of glucocorticoids. This lymphokine and the JAB1 protein form a complex in the cytosol near the peripheral plasma membrane, which may indicate an additional role in integrin signaling pathways. [provided by RefSeq, Jul 2008]

Uniprot Description

MIF: a lymphokine involved in cell-mediated immunity, immunoregulation, and inflammation. It plays a role in the regulation of macrophage function in host defense through the suppression of anti-inflammatory effects of glucocorticoids. This lymphokine and the JAB1 protein form a complex in the cytosol near the peripheral plasma membrane, which may indicate an additional role in integrin signaling pathways. Also acts as a phenylpyruvate tautomerase.

Protein type: Amino Acid Metabolism - phenylalanine; Apoptosis; Amino Acid Metabolism - tyrosine; EC 5.3.3.12; Cytokine; EC 5.3.2.1; Cell development/differentiation; Isomerase

Chromosomal Location of Human Ortholog: 22q11.23

Cellular Component: nucleoplasm; extracellular space; cell surface; cytoplasm; extracellular region; vesicle

Molecular Function: phenylpyruvate tautomerase activity; protein binding; hematopoietin/interferon-class (D200-domain) cytokine receptor binding; cytokine activity; dopachrome isomerase activity; receptor binding; chemoattractant activity

Biological Process: DNA damage response, signal transduction by p53 class mediator; regulation of macrophage activation; positive regulation of lipopolysaccharide-mediated signaling pathway; carboxylic acid metabolic process; cell aging; negative regulation of cellular protein metabolic process; prostaglandin biosynthetic process; positive regulation of peptidyl-serine phosphorylation; negative regulation of DNA damage response, signal transduction by p53 class mediator; positive regulation of MAP kinase activity; cell proliferation; positive regulation of fibroblast proliferation; negative regulation of myeloid cell apoptosis; positive regulation of peptidyl-tyrosine phosphorylation; negative regulation of mature B cell apoptosis; cell surface receptor linked signal transduction; positive chemotaxis; positive regulation of B cell proliferation; innate immune response; inflammatory response; positive regulation of phosphorylation; positive regulation of cytokine secretion; negative regulation of apoptosis

Disease: Rheumatoid Arthritis, Systemic Juvenile