Rabbit BAG-1 Polyclonal Antibody | anti-BAG-1 antibody

BAG-1 Antibody

Cat. Num. AAA9403303

• Gene Names: BAG1; HAP; BAG-1; RAP46
• Reactivity: Human, Mouse, Rat
• Applications: ELISA, Western Blot, Immunohistochemistry
• Purity: Affinity chromatography purified via peptide column
• Synonyms: BAG-1; Polyclonal Antibody; BAG-1 Antibody; Bcl-2-binding athanogene-1; receptor-associated protein 46; RAP46; anti-BAG-1 antibody

• Host: Rabbit
• Reactivity: Human, Mouse, Rat
• Clonality: Polyclonal
• Purity/Purification: Affinity chromatography purified via peptide column
• Form/Format: Supplied in PBS containing 0.02% sodium azide.
• Sequence Length: 345

• Applicable Applications for anti-BAG-1 antibody: ELISA (EIA), Western Blot (WB), Immunohistochemistry (IHC)
• Immunogen Type: Peptide
• Immunogen Description: Raised against a 14 amino acid peptide from near the amino terminus of human BAG-1.
• Target Name: BAG-1
• Preparation and Storage: Can be stored at -20 degree C, stable for one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

Western Blot (WB)

(Western blot analysis of BAG-1 in PC-3 cell lysate with BAG-1 antibody at (A) 1 and (B) 2 ug/mL.)

Immunohistochemistry (IHC)

(Immunohistochemistry of BAG-1 in human lymph node tissue with BAG-1 antibody at 2 ug/mL)

Related Product Information for anti-BAG-1 antibody

Bcl-2-associated athanogene 1 (BAG-1) was first identified as an anti-apoptotic bcl-2-binding protein. Later it was found to bind the molecular chaperones Hsp70 and Hsc70 through its carboxy-terminal sequence (termed the Bag domain), resulting in the inhibition of the refolding activity of these chaperones. It is thought that by binding and inhibiting these molecular chaperones, BAG-1is able to modulate the expression level of proteins requiring chaperones to fold correctly. One such group of proteins that are affected is glucocorticoid receptors. Other reports have suggested that the level of BAG-1 expression correlates with the aggressiveness of various cancers. Multiple isoforms of BAG-1 are known to exist.

Product Categories/Family for anti-BAG-1 antibody

Total protein Ab

NCBI and Uniprot Product Information

• NCBI GI #: 288915525
• NCBI GeneID: 573
• NCBI Accession #: NP_004314
• NCBI GenBank Nucleotide #: NM_004323.5
• Molecular Weight: 25,989 Da
• NCBI Official Full Name: BAG family molecular chaperone regulator 1 isoform BAG-1L
• NCBI Official Synonym Full Names: BCL2-associated athanogene
• NCBI Official Symbol: BAG1
• NCBI Official Synonym Symbols: HAP; BAG-1; RAP46
• NCBI Protein Information: BAG family molecular chaperone regulator 1
• UniProt Protein Name: BAG family molecular chaperone regulator 1
• Protein Family: BAG family molecular chaperone regulator
• UniProt Gene Name: BAG1
• UniProt Synonym Gene Names: HAP; BAG-1
• UniProt Entry Name: BAG1_HUMAN

NCBI Description

The oncogene BCL2 is a membrane protein that blocks a step in a pathway leading to apoptosis or programmed cell death. The protein encoded by this gene binds to BCL2 and is referred to as BCL2-associated athanogene. It enhances the anti-apoptotic effects of BCL2 and represents a link between growth factor receptors and anti-apoptotic mechanisms. Multiple protein isoforms are encoded by this mRNA through the use of a non-AUG (CUG) initiation codon, and three alternative downstream AUG initiation codons. A related pseudogene has been defined on chromosome X. [provided by RefSeq, Feb 2010]

Uniprot Description

BAG1: Inhibits the chaperone activity of HSP70/HSC70 by promoting substrate release. Inhibits the pro-apoptotic function of PPP1R15A, and has anti-apoptotic activity. Markedly increases the anti-cell death function of BCL2 induced by various stimuli. Homodimer. Forms a heteromeric complex with HSP70/HSC70. Binds to the ATPase domain of HSP/HSC70 chaperones. Isoform 1, isoform 3 and isoform 4 but not isoform 2 interact with HSPA8/HSC70. Interacts with NR3C1. Interacts with the N-terminal region of STK19. Interacts with PPP1R15A. Interacts with BCL2 in an ATP-dependent manner. Isoform 2 does not interact with BCL2. Up-regulated during differentiation of bladder epithelial cells and down-regulated during differentiation of prostate epithelium. Isoform 4 is the most abundantly expressed isoform. It is ubiquitously expressed throughout most tissues, except the liver, colon, breast and uterine myometrium. Isoform 1 is expressed in the ovary and testis. Isoform 4 is expressed in several types of tumor cell lines, and at consistently high levels in leukemia and lymphoma cell lines. Isoform 1 is expressed in the prostate, breast and leukemia cell lines. Isoform 3 is the least abundant isoform in tumor cell lines. 4 isoforms of the human protein are produced by alternative splicing.

Protein type: Motility/polarity/chemotaxis; Nuclear receptor co-regulator

Chromosomal Location of Human Ortholog: 9p12

Cellular Component: cytoplasm; nucleus

Molecular Function: protein binding; chaperone binding; receptor signaling protein activity

Biological Process: cell surface receptor linked signal transduction; apoptosis; negative regulation of apoptosis