Western Blot (WB)
(Western blot analysis of GOLPH1 in K562 cell lysate with GOLPH1 antibody at (A) 1 and (B) 2 ug/mL.)
Rabbit anti-Human GOLPH1 Polyclonal Antibody | anti-GOLPH1 antibody
GOLPH1 Antibody
Gene Names
ACBD3; PAP7; GCP60; GOCAP1; GOLPH1
Reactivity
Human
Applications
ELISA, Western Blot
Purity
Affinity chromatography purified via peptide column
Synonyms
GOLPH1; Polyclonal Antibody; GOLPH1 Antibody; Golgi phosphoprotein 1; golgi complex-associated protein 1; GOCAP1; GCP60; acyl CoA binding domain containing 3; ACBD3; anti-GOLPH1 antibody
Host
Rabbit
Reactivity
Human
Clonality
Polyclonal
Purity/Purification
Affinity chromatography purified via peptide column
Form/Format
Supplied in PBS containing 0.02% sodium azide.
Sequence Length
528
Applicable Applications for anti-GOLPH1 antibody
ELISA (EIA), Western Blot (WB)
Immunogen Type
Peptide
Immunogen Description
Raised against a 16 amino acid peptide from near the amino terminus of human GOLPH1.
Target Name
GOLPH1
Preparation and Storage
Can be stored at -20 degree C, stable for one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Western Blot (WB)
(Western blot analysis of GOLPH1 in K562 cell lysate with GOLPH1 antibody at (A) 1 and (B) 2 ug/mL.)
Western Blot (WB)
(Western blot analysis of GOLPH1 in K562 cell lysate with GOLPH1 antibody at (A) 1 and (B) 2 ug/mL.)
Related Product Information for anti-GOLPH1 antibody
GOLPH1, also known as GCP60, was initially identified as a Golgi protein that can interact with the integral membrane protein giantin and is thought to be involved in the maintenance of the Golgi structure. GOLPH1 has also been shown to interact with other Golgi proteins such as Golgin-160, a Golgi protein that can be cleaved by caspases-2, -3, and -7, leading to the nuclear localization of Golgin-160. GOLPH1 interaction with the Golgin-160 fragments is stronger than that with the intact Golgin-160, with its interaction regulated by the oxidation state of Cys-463 within GOLPH1, suggesting that the nuclear localization of the caspase-cleaved Golgin-160 fragments is a highly coordinated event. GOLPH1 has also been found to interact with Numb, a cytosolic signaling protein that mediates asymetric cell division of neural progenitor cells to a daughter progenitor cell and a neuron, suggesting that Golgi fragmentation and reconstitution during the cell cycle differentially regulate Numb signaling through changes in GOLPH1 subcellular distribution and may couple cell fate with cell cycle progression.
Product Categories/Family for anti-GOLPH1 antibody
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
Molecular Weight
60,593 Da
NCBI Official Full Name
acyl-Coenzyme A binding domain containing 3, isoform CRA_a
NCBI Official Synonym Full Names
acyl-CoA binding domain containing 3
NCBI Official Symbol
ACBD3
NCBI Official Synonym Symbols
PAP7; GCP60; GOCAP1; GOLPH1
NCBI Protein Information
Golgi resident protein GCP60
UniProt Protein Name
Golgi resident protein GCP60
UniProt Gene Name
ACBD3
UniProt Synonym Gene Names
GCP60; GOCAP1; GOLPH1; GOCAP1; GOLPH1
UniProt Entry Name
GCP60_HUMAN
NCBI Description
The Golgi complex plays a key role in the sorting and modification of proteins exported from the endoplasmic reticulum. The protein encoded by this gene is involved in the maintenance of Golgi structure and function through its interaction with the integral membrane protein giantin. It may also be involved in the hormonal regulation of steroid formation. [provided by RefSeq, Jul 2008]
Uniprot Description
ACBD3: Involved in the maintenance of Golgi structure by interacting with giantin, affecting protein transport between the endoplasmic reticulum and Golgi. Involved in hormone-induced steroid biosynthesis in testicular Leydig cells.
Chromosomal Location of Human Ortholog: 1q42.12
Cellular Component: Golgi membrane; Golgi apparatus; membrane; mitochondrion; integral to membrane
Molecular Function: protein binding; acyl-CoA binding
Biological Process: transport; steroid biosynthetic process
Research Articles on GOLPH1
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Product Notes
The GOLPH1 acbd3 (Catalog #AAA9401723) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The GOLPH1 Antibody reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's GOLPH1 can be used in a range of immunoassay formats including, but not limited to, ELISA (EIA), Western Blot (WB). Researchers should empirically determine the suitability of the GOLPH1 acbd3 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "GOLPH1, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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