Loading...

Skip to main content

Call us on + 1 (800) 604-9114 for more information about our products

Looking for specific datasheet Manual/COA/MSDS?
Request a Manual/COA/MSDS

Interested to get a quote about our products?
Request a Quote

Western Blot (WB) (Western blot analysis of extracts of various cell lines, using ASNS antibody.)

Rabbit ASNS Polyclonal Antibody | anti-ASNS antibody

ASNS Antibody

Gene Names
ASNS; TS11; ASNSD
Reactivity
Human, Mouse, Rat
Applications
Western Blot, Immunohistochemistry, Immunofluorescence
Purity
Antibodies were purified by affinity purification using immunogen.
Synonyms
ASNS; Polyclonal Antibody; ASNS Antibody; TS11; ASNSD; anti-ASNS antibody
Ordering
For Research Use Only!
Host
Rabbit
Reactivity
Human, Mouse, Rat
Clonality
Polyclonal
Specificity
The antibody detects endogenous level of total ASNS protein.
Purity/Purification
Antibodies were purified by affinity purification using immunogen.
Form/Format
Supplied at 1.0mg/mL in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Concentration
1.0 mg/ml (varies by lot)
Sequence Length
540
Applicable Applications for anti-ASNS antibody
Western Blot (WB), Immunohistochemistry (IHC), Immunofluorescence (IF)
Application Notes
Western blotting: 1:500 - 1:2000
Immunohistochemistry: 1:50 - 1:200
Immunofluorescence: 1:50 - 1:200
Immunogen Type
Recombinant Protein
Immunogen Description
Recombinant protein of human ASNS.
Target Name
ASNS
Preparation and Storage
Store at -20 degree C

Western Blot (WB)

(Western blot analysis of extracts of various cell lines, using ASNS antibody.)

Western Blot (WB) (Western blot analysis of extracts of various cell lines, using ASNS antibody.)

Immunohistochemistry (IHC)

(Immunohistochemical analysis of paraffin-embedded mouse pancreas using ASNS antibody at dilution of 1:200 (400x lens).)

Immunohistochemistry (IHC) (Immunohistochemical analysis of paraffin-embedded mouse pancreas using ASNS antibody at dilution of 1:200 (400x lens).)

Immunofluorescence (IF)

(Immunofluorescence analysis of A549 cell using ASNS antibody. Blue: DAPI for nuclear staining.)

Immunofluorescence (IF) (Immunofluorescence analysis of A549 cell using ASNS antibody. Blue: DAPI for nuclear staining.)
Related Product Information for anti-ASNS antibody
The protein encoded by this gene is involved in the synthesis of asparagine. This gene complements a mutation in the temperature-sensitive hamster mutant ts11, which blocks progression through the G1 phase of the cell cycle at nonpermissive temperature. Alternatively spliced transcript variants have been described for this gene.
Product Categories/Family for anti-ASNS antibody

NCBI and Uniprot Product Information

NCBI GI #
NCBI GeneID
440
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
54,818 Da
NCBI Official Full Name
asparagine synthetase
NCBI Official Synonym Full Names
asparagine synthetase (glutamine-hydrolyzing)
NCBI Official Symbol
ASNS
NCBI Official Synonym Symbols
TS11; ASNSD
NCBI Protein Information
asparagine synthetase [glutamine-hydrolyzing]
UniProt Protein Name
Asparagine synthetase [glutamine-hydrolyzing]
Protein Family
UniProt Gene Name
ASNS
UniProt Synonym Gene Names
TS11
UniProt Entry Name
ASNS_HUMAN

NCBI Description

The protein encoded by this gene is involved in the synthesis of asparagine. This gene complements a mutation in the temperature-sensitive hamster mutant ts11, which blocks progression through the G1 phase of the cell cycle at nonpermissive temperature. Alternatively spliced transcript variants have been described for this gene. [provided by RefSeq, May 2010]

Uniprot Description

ASNS: 3 isoforms of the human protein are produced by alternative splicing

Protein type: Amino Acid Metabolism - alanine, aspartate and glutamate; Energy Metabolism - nitrogen; Cell cycle regulation; EC 6.3.5.4; Ligase

Chromosomal Location of Human Ortholog: 7q21.3

Cellular Component: cytosol

Molecular Function: protein homodimerization activity; asparagine synthase (glutamine-hydrolyzing) activity; cofactor binding; ATP binding

Biological Process: response to light stimulus; unfolded protein response; response to toxin; glutamine metabolic process; liver development; positive regulation of mitotic cell cycle; response to amino acid stimulus; cellular response to hormone stimulus; cellular response to glucose starvation; asparagine biosynthetic process; unfolded protein response, activation of signaling protein activity; cellular protein metabolic process; response to mechanical stimulus; amino acid biosynthetic process; response to methotrexate; response to follicle-stimulating hormone stimulus; negative regulation of apoptosis

Disease: Asparagine Synthetase Deficiency

Research Articles on ASNS

Similar Products

Product Notes

The ASNS asns (Catalog #AAA9400730) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The ASNS Antibody reacts with Human, Mouse, Rat and may cross-react with other species as described in the data sheet. AAA Biotech's ASNS can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunohistochemistry (IHC), Immunofluorescence (IF). Western blotting: 1:500 - 1:2000 Immunohistochemistry: 1:50 - 1:200 Immunofluorescence: 1:50 - 1:200. Researchers should empirically determine the suitability of the ASNS asns for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "ASNS, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.

Precautions

All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.

Disclaimer

Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.

Item has been added to Shopping Cart

If you are ready to order, navigate to Shopping Cart and get ready to checkout.

Looking for a specific manual?
Request a Manual

Request more Information

Please complete the form below and a representative will contact you as soon as possible.

Request a Manual

Please complete the form below and a representative will contact you as soon as possible.

Request a Quote

Please complete the form below and a representative will contact you as soon as possible.