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Western Blot (WB) (ASNS Antibody (N-term) western blot analysis in WiDr cell line lysates (35ug/lane).This demonstrates the ASNS antibody detected the ASNS protein (arrow).)

Rabbit ASNS Polyclonal Antibody | anti-ASNS antibody

ASNS Antibody (N-term)

Gene Names
ASNS; TS11; ASNSD
Reactivity
Human, mouse (Predicted Reactivity: Bovine, Hamster, Rat)
Applications
Western Blot, ELISA
Purity
Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)
Synonyms
ASNS; Polyclonal Antibody; ASNS Antibody (N-term); Asparagine synthetase [glutamine-hydrolyzing]; Cell cycle control protein TS11; Glutamine-dependent asparagine synthetase; TS11; anti-ASNS antibody
Ordering
For Research Use Only!
Host
Rabbit
Reactivity
Human, mouse (Predicted Reactivity: Bovine, Hamster, Rat)
Clonality
Polyclonal
Isotype
Rabbit Ig
Specificity
This ASNS antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 120-147 amino acids from the N-terminal region of human ASNS.
Purity/Purification
Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)
Form/Format
Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
Concentration
Vial Concentration: 0.5 (varies by lot)
Sequence Positions
120-147
Sequence Length
540
Applicable Applications for anti-ASNS antibody
Western Blot (WB), ELISA (EIA)
Application Notes
WB~~1:1000
Antigen Type
Synthetic Peptide
Antigen Source
HUMAN
Preparation and Storage
Maintain refrigerated at 2-8 degree C for up to 6 months. For long term storage store at -20 degree C in small aliquots to prevent freeze-thaw cycles.

Western Blot (WB)

(ASNS Antibody (N-term) western blot analysis in WiDr cell line lysates (35ug/lane).This demonstrates the ASNS antibody detected the ASNS protein (arrow).)

Western Blot (WB) (ASNS Antibody (N-term) western blot analysis in WiDr cell line lysates (35ug/lane).This demonstrates the ASNS antibody detected the ASNS protein (arrow).)

Western Blot (WB)

(ASNS Antibody (N-term) western blot analysis in mouse testis tissue lysates (35ug/lane).This demonstrates the ASNS antibody detected the ASNS protein (arrow).)

Western Blot (WB) (ASNS Antibody (N-term) western blot analysis in mouse testis tissue lysates (35ug/lane).This demonstrates the ASNS antibody detected the ASNS protein (arrow).)
Related Product Information for anti-ASNS antibody
The protein encoded by this gene is involved in the
synthesis of asparagine. This gene complements a mutation in the
temperature-sensitive hamster mutant ts11, which blocks progression
through the G1 phase of the cell cycle at nonpermissive
temperature. Alternatively spliced transcript variants have been
described for this gene.
Product Categories/Family for anti-ASNS antibody
References
Akagi, T., et al. Leuk. Res. 33(7):991-996(2009)
Hashimoto, K., et al. Anticancer Res. 29(4):1303-1308(2009)
Su, N., et al. J. Biol. Chem. 283(50):35106-35117(2008)
Luo, C.Y., et al. Zhonghua Xue Ye Xue Za Zhi 29(7):446-449(2008)
Heng, H.H., et al. Cytogenet. Cell Genet. 66(2):135-138(1994)

NCBI and Uniprot Product Information

NCBI GI #
NCBI GeneID
440
NCBI GenBank Nucleotide #
Molecular Weight
64370
NCBI Official Full Name
asparagine synthetase
NCBI Official Synonym Full Names
asparagine synthetase (glutamine-hydrolyzing)
NCBI Official Symbol
ASNS
NCBI Official Synonym Symbols
TS11; ASNSD
NCBI Protein Information
asparagine synthetase [glutamine-hydrolyzing]
UniProt Protein Name
Asparagine synthetase [glutamine-hydrolyzing]
Protein Family
UniProt Gene Name
ASNS
UniProt Synonym Gene Names
TS11
UniProt Entry Name
ASNS_HUMAN

NCBI Description

The protein encoded by this gene is involved in the synthesis of asparagine. This gene complements a mutation in the temperature-sensitive hamster mutant ts11, which blocks progression through the G1 phase of the cell cycle at nonpermissive temperature. Alternatively spliced transcript variants have been described for this gene. [provided by RefSeq, May 2010]

Uniprot Description

ASNS: 3 isoforms of the human protein are produced by alternative splicing

Protein type: Cell cycle regulation; Ligase; EC 6.3.5.4; Energy Metabolism - nitrogen; Amino Acid Metabolism - alanine, aspartate and glutamate

Chromosomal Location of Human Ortholog: 7q21.3

Cellular Component: cytosol

Molecular Function: protein homodimerization activity; asparagine synthase (glutamine-hydrolyzing) activity; cofactor binding; ATP binding

Biological Process: response to light stimulus; unfolded protein response; response to toxin; glutamine metabolic process; liver development; response to amino acid stimulus; positive regulation of mitotic cell cycle; cellular response to hormone stimulus; cellular response to glucose starvation; cellular protein metabolic process; asparagine biosynthetic process; unfolded protein response, activation of signaling protein activity; response to mechanical stimulus; response to methotrexate; amino acid biosynthetic process; response to follicle-stimulating hormone stimulus; negative regulation of apoptosis

Disease: Asparagine Synthetase Deficiency

Research Articles on ASNS

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Product Notes

The ASNS asns (Catalog #AAA9210300) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The immunogen sequence is 120-147. The ASNS Antibody (N-term) reacts with Human, mouse (Predicted Reactivity: Bovine, Hamster, Rat) and may cross-react with other species as described in the data sheet. AAA Biotech's ASNS can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), ELISA (EIA). WB~~1:1000. Researchers should empirically determine the suitability of the ASNS asns for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "ASNS, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.

Precautions

All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.

Disclaimer

Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.

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