Mouse angiopoietin-like 3 ELISA Kit | ANGPTL3 elisa kit

Mouse Angiopoietin-related protein 3, ANGPTL3 ELISA Kit

Cat. Num. AAA938852

• Gene Names: Angptl3; hypl
• Reactivity: Mouse
• Synonyms: angiopoietin-like 3; Mouse Angiopoietin-related protein 3; ANGPTL3 ELISA Kit; Mouse Angiopoietin-related protein 3 (ANGPTL3) ELISA kit; ANGPT5; angiopoietin 5; angiopoietin-related protein 3; Mouse Angiopoietin-related protein 3 (ANGPTL3); ANGPTL3 elisa kit

• Reactivity: Mouse
• Specificity: This assay has high sensitivity and excellent specificity for detection of mouse ANGPTL3. No significant cross-reactivity or interference between mouse ANGPTL3 and analogues was observed.
• Sequence Length: 455

• Samples: Serum, plasma, tissue homogenates.
• Assay Type: Sandwich
• Detection Range: 31.25 pg/ml -2000 pg/ml.
• Sensitivity: 10.24 pg/ml.
• Intra-assay Precision: Intra-assay Precision (Precision within an assay): CV%<8%. Three samples of known concentration were tested twenty times on one plate to assess.
• Inter-assay Precision: Inter-assay Precision (Precision between assays): CV%<10%. Three samples of known concentration were tested in twenty assays to assess.
• Preparation and Storage: Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.

Related Product Information for ANGPTL3 elisa kit

Principle of the Assay||This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for ANGPTL3 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any ANGPTL3 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for ANGPTL3 is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of ANGPTL3 bound in the initial step. The color development is stopped and the intensity of the color is measured.

NCBI and Uniprot Product Information

• NCBI GI #: 33469117
• NCBI GeneID: 30924
• NCBI Accession #: NP_038941.1
• NCBI GenBank Nucleotide #: NM_013913.3
• UniProt Accession #: Q9R182
• Molecular Weight: 52,543 Da
• NCBI Official Full Name: angiopoietin-related protein 3
• NCBI Official Synonym Full Names: angiopoietin-like 3
• NCBI Official Symbol: Angptl3
• NCBI Official Synonym Symbols: hypl
• NCBI Protein Information: angiopoietin-related protein 3; hypolipidemia; angiopoietin-like protein 3
• UniProt Protein Name: Angiopoietin-related protein 3
• Protein Family: Angiopoietin-related protein
• UniProt Gene Name: Angptl3
• UniProt Entry Name: ANGL3_MOUSE

NCBI Description

This gene encodes a member of the angiopoietin-like family of proteins. The encoded preproprotein is proteolytically processed to generate multiple protein products, which may inhibit triglyceride metabolism. Homozygous knockout mice for this gene exhibit reduced plasma lipid concentrations, including reduced plasma triglyceride concentrations, and enhanced activity of enzymes involved in triglyceride metabolism. [provided by RefSeq, Aug 2015]

Uniprot Description

ANGPTL3: Defects in ANGPTL3 are the cause of familial hypobetalipoproteinemia type 2 (FHBL2); also called combined hypobetalipoproteinemia familial. FHBL2 is a disorder of lipid metabolism characterized by less than 5th percentile age- and sex-specific levels of low density lipoproteins, and dietary fat malabsorption. Affected individuals present with combined hypolipidemia, consisting of extremely low plasma levels of LDL cholesterol, HDL cholesterol, and triglycerides.

Protein type: Secreted, signal peptide; Inhibitor; Motility/polarity/chemotaxis; Secreted; Cell adhesion

Cellular Component: Golgi apparatus; extracellular space; cell surface; early endosome; extracellular region

Molecular Function: enzyme inhibitor activity; integrin binding; phospholipase inhibitor activity; growth factor activity

Biological Process: cholesterol metabolic process; glycerol metabolic process; phospholipid catabolic process; cell-matrix adhesion; lipid homeostasis; positive regulation of lipid catabolic process; fatty acid metabolic process; signal transduction; sequestering of lipid; cholesterol homeostasis; positive regulation of angiogenesis; triacylglycerol metabolic process; acylglycerol homeostasis; phospholipid metabolic process; negative regulation of catalytic activity; positive regulation of lipid metabolic process; artery morphogenesis; negative regulation of lipoprotein lipase activity; positive regulation of cell migration; phospholipid homeostasis