Rabbit anti-Human, Mouse IL-4R alpha Antibody | anti-IL-4R alpha antibody

IL-4R alpha Antibody

Cat. Num. AAA9385543

• Gene Names: IL4R; CD124; IL4RA; IL-4RA
• Reactivity: Human, Mouse
• Applications: Western Blot, Immunohistochemistry
• Purity: Immunogen affinity purified
• Synonyms: IL-4R alpha; IL-4R alpha Antibody; CD124; IL 4R alpha; IL-4 receptor subunit alpha; IL-4-binding protein; IL-4R subunit alpha; IL-4R-alpha; IL-4RA; IL4-BP; Il4r; IL4R nirs variant 1; IL4RA; IL4RA_HUMAN; Interleukin 4 receptor alpha chain; Interleukin 4 receptor; sIL4Ralpha/prot; Soluble IL-4 receptor subunit alpha; Soluble IL-4R-alpha; Soluble interleukin-4 receptor subunit alpha; anti-IL-4R alpha antibody

• Host: Rabbit
• Reactivity: Human, Mouse
• Specificity: IL-4R alpha Antibody detects endogenous levels of total IL-4R alpha
• Purity/Purification: Immunogen affinity purified
• Concentration: 1.0mg/ml (varies by lot)
• Sequence Length: 825

• Applicable Applications for anti-IL-4R alpha antibody: Western Blot (WB), Immunohistochemistry (IHC)
• Application Notes: WB 1:1000-3000 IHC 1:200, ELISA(peptide) 1:20000-1:40000; Optimal dilutions/concentrations should be determined by the end user.
• Immunogen: A synthesized peptide derived from human IL-4R alpha
• Post-translational Modifications: On IL4 binding, phosphorylated on C-terminal tyrosine residues. Phosphorylation on any one of tyrosine residues, Tyr-575, Tyr-603 or Tyr-631, is required for STAT6-induced gene induction.The soluble form (sIL4R/IL4BP) can also be produced by proteolytic cleavage at the cell surface (shedding) by a metalloproteinase.
• Tissue Specificity: Isoform 1 and isoform 2 are highly expressed in activated T-cells.
• Subunit Structure: The functional IL4 receptor is formed by initial binding of IL4 to IL4R. Subsequent recruitment to the complex of the common gamma chain, in immune cells, creates a type I receptor and, in non-immune cells, of IL13RA1 forms a type II receptor. IL4R can also interact with the IL13/IL13RA1 complex to form a similar type II receptor (PubMed:7775445, PubMed:8804422). Interacts with PIK3C3 (By similarity). Interacts with the SH2-containing phosphatases, PTPN6/SHIP1, PTPN11/SHIP2 and INPP5D/SHIP (PubMed:11714803). Interacts with JAK1 through a Box 1-containing region; inhibited by SOCS5. Interacts with SOCS5; inhibits IL4 signaling (By similarity). Interacts with JAK3 (PubMed:7538655). Interacts with CLM1 (By similarity).
• Similarity: The extracellular domain represents the IL4 binding protein (IL4BP).The WSXWS motif appears to be necessary for proper protein folding and thereby efficient intracellular transport and cell-surface receptor binding. The box 1 motif is required for JAK interaction and/or activation.Contains 1 copy of a cytoplasmic motif that is referred to as the immunoreceptor tyrosine-based inhibitor motif (ITIM). This motif is involved in modulation of cellular responses. The phosphorylated ITIM motif can bind the SH2 domain of several SH2-containing phosphatases.Belongs to the type I cytokine receptor family. Type 4 subfamily.
• Buffer: Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
• Preparation and Storage: Store at -20 °C.Stable for 12 months from date of receipt

Western blot

(Western blot analysis IL-4R alpha using MBS9385543 whole cell lysates)

Related Product Information for anti-IL-4R alpha antibody

Receptor for both interleukin 4 and interleukin 13. Couples to the JAK1/2/3-STAT6 pathway. The IL4 response is involved in promoting Th2 differentiation. The IL4/IL13 responses are involved in regulating IgE production and, chemokine and mucus production at sites of allergic inflammation. In certain cell types, can signal through activation of insulin receptor substrates, IRS1/IRS2.

NCBI and Uniprot Product Information

• NCBI GI #: 4557669
• NCBI GeneID: 3566
• NCBI Accession #: NP_000409.1
• NCBI GenBank Nucleotide #: NM_000418.3
• UniProt Accession #: P24394; Q96P01; Q9H181; Q9H182; Q9H183; Q9H184; Q9H185; Q9H186; Q9H187; B4E076; B9EKU8; H3BSY5
• Molecular Weight: 88,267 Da
• NCBI Official Full Name: interleukin-4 receptor subunit alpha isoform a
• NCBI Official Synonym Full Names: interleukin 4 receptor
• NCBI Official Symbol: IL4R
• NCBI Official Synonym Symbols: CD124; IL4RA; IL-4RA
• NCBI Protein Information: interleukin-4 receptor subunit alpha
• UniProt Protein Name: Interleukin-4 receptor subunit alpha
• UniProt Gene Name: IL4R
• UniProt Synonym Gene Names: IL4RA; IL-4 receptor subunit alpha; IL-4R subunit alpha; IL-4R-alpha; IL-4RA; Soluble IL-4 receptor subunit alpha; Soluble IL-4R-alpha; sIL4Ralpha/prot; IL4-BP

NCBI Description

This gene encodes the alpha chain of the interleukin-4 receptor, a type I transmembrane protein that can bind interleukin 4 and interleukin 13 to regulate IgE production. The encoded protein also can bind interleukin 4 to promote differentiation of Th2 cells. A soluble form of the encoded protein can be produced by proteolysis of the membrane-bound protein, and this soluble form can inhibit IL4-mediated cell proliferation and IL5 upregulation by T-cells. Allelic variations in this gene have been associated with atopy, a condition that can manifest itself as allergic rhinitis, sinusitus, asthma, or eczema. Polymorphisms in this gene are also associated with resistance to human immunodeficiency virus type-1 infection. Alternate splicing results in multiple transcript variants. [provided by RefSeq, Apr 2012]

Uniprot Description

IL4R: Receptor for both interleukin 4 and interleukin 13. Couples to the JAK1/2/3-STAT6 pathway. The IL4 response is involved in promoting Th2 differentiation. The IL4/IL13 responses are involved in regulating IgE production and, chemokine and mucus production at sites of allergic inflammation. In certain cell types, can signal through activation of insulin receptor substrates, IRS1/IRS2. Belongs to the type I cytokine receptor family. Type 4 subfamily. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Membrane protein, integral; Receptor, cytokine

Chromosomal Location of Human Ortholog: 16p12.1

Cellular Component: integral to plasma membrane; receptor complex

Molecular Function: interleukin-4 receptor activity; protein binding; receptor signaling protein activity

Biological Process: immune response; signal transduction

Disease: Human Immunodeficiency Virus Type 1, Susceptibility To; Ige Responsiveness, Atopic