Human keratin 32 ELISA Kit | KRT32 elisa kit
Human Keratin, type I cuticular Ha2, KRT32 ELISA Kit
Gene Names
KRT32; HA2; HKA2; hHa2; KRTHA2
Reactivity
Human
Synonyms
keratin 32; Human Keratin; type I cuticular Ha2; KRT32 ELISA Kit; type I cuticular Ha2 (KRT32) ELISA kit; HA2; HKA2; KRTHA2; hHa2; hard keratin; type I; 2; keratin; hair; acidic; type I cuticular hair keratin; KRT32 elisa kit
Reactivity
Human
Specificity
No significant cross-reactivity or interference between Human KRT32 and analogues was observed.
Samples
Serum, Plasma, Tissue Homogenate, Feces and Urine
Assay Type
Sandwich
Detection Range
0.625 ng/ml - 20 ng/ml.
Sensitivity
0.1 ng/ml.
Intended Uses
This Quantitative Sandwich ELISA kit is only for in vitro research use only, not for drug, household, therapeutic applications! It is intended to be determinated KRT32 concentrations in Human serum, plasma and other body fluids. Using Purified Human KRT32 antibody to coat Microelisa Stripplate wells to make solid-phase antibody, then add KRT32 and KRT32 antibody which has been labeled with HRP to wells, then the reactants become antibody-antigen-antibody-enzyme complex, after washing completely, add TMB substrate solution, TMB substrate becomes blue color under HRP enzyme-catalyzed, reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm. The concentration of KRT32 in the samples is then determined by comparing the O.D. of the samples to the standard curve.
Intra-assay Precision
Intra-assay CV (%) is less than 15%.
Inter-assay Precision
Inter-assay CV (%) is less than 15%. [CV(%) = SD/mean ×100]
Preparation and Storage
Store all reagents at 2-8 degree C
Related Product Information for KRT32 elisa kit
Background: This Quantitative Sandwich ELISA kit is only for in vitro research use only! It is intended to be determinated KRT32 concentrations in Human serum, plasma and other body fluids. Using Purified Human KRT32 antibody to coat Microelisa Stripplate wells to make solid-phase antibody, then add KRT32 and KRT32 antibody which has been labeled with HRP to wells, then the reactants become antibody-antigen-antibody-enzyme complex, after washing completely, add TMB substrate solution, TMB substrate becomes blue color under HRP enzyme-catalyzed, reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm. The concentration of KRT32 in the samples is then determined by comparing the O.D. of the samples to the standard curve.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
Molecular Weight
50,343 Da
NCBI Official Full Name
keratin, type I cuticular Ha2
NCBI Official Synonym Full Names
keratin 32
NCBI Official Symbol
KRT32
NCBI Official Synonym Symbols
HA2; HKA2; hHa2; KRTHA2
NCBI Protein Information
keratin, type I cuticular Ha2; K32; keratin-32; hard keratin, type I, 2; hair keratin, type I Ha2; keratin, hair, acidic, 2; type I cuticular hair keratin
UniProt Protein Name
Keratin, type I cuticular Ha2
Protein Family
UniProt Gene Name
KRT32
UniProt Synonym Gene Names
HHA2; HKA2; KRTHA2; K32
UniProt Entry Name
K1H2_HUMAN
NCBI Description
The protein encoded by this gene is a member of the keratin gene family. As a type I hair keratin, it is an acidic protein which heterodimerizes with type II keratins to form hair and nails. The type I hair keratins are clustered in a region of chromosome 17q12-q21 and have the same direction of transcription. [provided by RefSeq, Jul 2008]
Uniprot Description
K32: a type I hair keratin. The keratins are intermediate filament proteins responsible for the structural integrity of epithelial cells and are subdivided into cytokeratins and hair keratins. There are two types of hair/microfibrillar keratin: type I (acidic; 40-55 kDa) [K9 to K20] and type II (neutral to basic; 56-70 kDa) [K1 to K8]. Both a basic and an acidic keratin are required for filament assembly.
Protein type: Cytoskeletal; Motility/polarity/chemotaxis
Chromosomal Location of Human Ortholog: 17q21.2
Cellular Component: intermediate filament
Molecular Function: structural molecule activity
Biological Process: epidermis development
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Product Notes
The Human KRT32 krt32 (Catalog #AAA9341130) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA9341130 ELISA Kit recognizes Human KRT32. It is sometimes possible for the material contained within the vial of "keratin 32, ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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