Mouse gamma-aminobutyric acid (GABA) A receptor, alpha 1 ELISA Kit | GABRA1 elisa kit

Mouse Gamma-aminobutyric acid receptor subunit alpha-1, GABRA1 ELISA Kit

Cat. Num. AAA9339158

• Gene Names: Gabra1; Gabra-1
• Reactivity: Mouse
• Synonyms: gamma-aminobutyric acid (GABA) A receptor; alpha 1; Mouse Gamma-aminobutyric acid receptor subunit alpha-1; GABRA1 ELISA Kit; Mouse Gamma-aminobutyric acid receptor subunit alpha-1 (GABRA1) ELISA kit; ECA4; EJM; EJM5; ; GABRA1 elisa kit

• Reactivity: Mouse
• Specificity: No significant cross-reactivity or interference between Mouse GABRA1 and analogues was observed.

• Samples: Serum, Plasma, Tissue Homogenate, Feces and Urine
• Assay Type: Sandwich
• Detection Range: 0.5mumol/L-16mumol/L.
• Sensitivity: 0.1mumol/L.
• Intended Uses: This Quantitative Sandwich ELISA kit is only for in vitro research use only, not for drug, household, therapeutic applications! It is intended to be determinated GABRA1 concentrations in Human serum, plasma and other body fluids. Using Purified Human GABRA1 antibody to coat Microelisa Stripplate wells to make solid-phase antibody, then add GABRA1 and GABRA1 antibody which has been labeled with HRP to wells, then the reactants become antibody-antigen-antibody-enzyme complex, after washing completely, add TMB substrate solution, TMB substrate becomes blue color under HRP enzyme-catalyzed, reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm. The concentration of GABRA1 in the samples is then determined by comparing the O.D. of the samples to the standard curve.
• Intray-assay Precision: Intra-assay CV (%) is less than 15%.
• Inter-assay Precision: Inter-assay CV (%) is less than 15%. [CV(%) = SD/mean ×100]
• Preparation and Storage: Store all reagents at 2-8 degree C

Related Product Information for GABRA1 elisa kit

Background: This Quantitative Sandwich ELISA kit is only for in vitro research use only! It is intended to be determinated GABRA1 concentrations in Human serum, plasma and other body fluids. Using Purified Human GABRA1 antibody to coat Microelisa Stripplate wells to make solid-phase antibody, then add GABRA1 and GABRA1 antibody which has been labeled with HRP to wells, then the reactants become antibody-antigen-antibody-enzyme complex, after washing completely, add TMB substrate solution, TMB substrate becomes blue color under HRP enzyme-catalyzed, reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm. The concentration of GABRA1 in the samples is then determined by comparing the O.D. of the samples to the standard curve.

NCBI and Uniprot Product Information

• NCBI GI #: 6753936
• NCBI GeneID: 14394
• NCBI Accession #: NP_034380.1
• NCBI GenBank Nucleotide #: NM_010250.5
• UniProt Accession #: P62812; P18504
• Molecular Weight: 51,754 Da
• NCBI Official Full Name: gamma-aminobutyric acid receptor subunit alpha-1
• NCBI Official Synonym Full Names: gamma-aminobutyric acid (GABA) A receptor, subunit alpha 1
• NCBI Official Symbol: Gabra1
• NCBI Official Synonym Symbols: Gabra-1
• NCBI Protein Information: gamma-aminobutyric acid receptor subunit alpha-1; GABAA alpha 1; GABAAR alpha1; GABA(A) receptor subunit alpha-1; gamma-aminobutyric acid (GABA-A) receptor, subunit alpha 1
• UniProt Protein Name: Gamma-aminobutyric acid receptor subunit alpha-1
• Protein Family: Gamma-aminobutyric acid receptor
• UniProt Gene Name: Gabra1
• UniProt Synonym Gene Names: Gabra-1
• UniProt Entry Name: GBRA1_MOUSE

Uniprot Description

GABRA1: GABA, the major inhibitory neurotransmitter in the vertebrate brain, mediates neuronal inhibition by binding to the GABA/benzodiazepine receptor and opening an integral chloride channel. Defects in GABRA1 are the cause of childhood absence epilepsy type 4 (ECA4). A subtype of idiopathic generalized epilepsy characterized by onset at age 6-7 years, frequent absence seizures (several per day) and bilateral, synchronous, symmetric 3-Hz spike waves on EEG. During adolescence, tonic-clonic and myoclonic seizures may develop. Absence seizures may either remit or persist into adulthood. Defects in GABRA1 are the cause of juvenile myoclonic epilepsy type 5 (EJM5). A subtype of idiopathic generalized epilepsy. Patients have afebrile seizures only, with onset in adolescence (rather than in childhood) and myoclonic jerks which usually occur after awakening and are triggered by sleep deprivation and fatigue. Belongs to the ligand-gated ion channel (TC 1.A.9) family. Gamma-aminobutyric acid receptor (TC 1.A.9.5) subfamily. GABRA1 sub-subfamily.

Protein type: Transporter; Transporter, ion channel; Membrane protein, multi-pass; Membrane protein, integral; Channel, ligand-gated

Cellular Component: postsynaptic membrane; membrane; integral to plasma membrane; integral to membrane; plasma membrane; synapse; cell junction

Molecular Function: chloride channel activity; protein binding; GABA-A receptor activity; GABA receptor activity; ion channel activity; drug binding; extracellular ligand-gated ion channel activity

Biological Process: transport; synaptic transmission, GABAergic; ion transport; chloride transport; gamma-aminobutyric acid signaling pathway