Mouse DNA replication helicase 2 homolog (yeast) ELISA Kit | DNA2 elisa kit
Mouse DNA2-like helicase, DNA2 ELISA Kit
NCBI and Uniprot Product Information
Uniprot Description
DNA2: Key enzyme involved in DNA replication and DNA repair in nucleus and mitochondrion. Involved in Okazaki fragments processing by cleaving long flaps that escape FEN1: flaps that are longer than 27 nucleotides are coated by replication protein A complex (RPA), leading to recruit DNA2 which cleaves the flap until it is too short to bind RPA and becomes a substrate for FEN1. Also involved in 5'-end resection of DNA during double- strand break (DSB) repair: recruited by BLM and mediates the cleavage of 5'-ssDNA, while the 3'-ssDNA cleavage is prevented by the presence of RPA. Also involved in DNA replication checkpoint independently of Okazaki fragments processing. Possesses different enzymatic activities, such as single-stranded DNA (ssDNA)- dependent ATPase, 5'-3' helicase and endonuclease activities. While the ATPase and endonuclease activities are well-defined and play a key role in Okazaki fragments processing and DSB repair, the 5'-3' DNA helicase activity is subject to debate. According to various reports, the helicase activity is weak and its function remains largely unclear. Helicase activity may promote the motion of DNA2 on the flap, helping the nuclease function. Belongs to the DNA2/NAM7 helicase family. 4 isoforms of the human protein are produced by alternative splicing.
Protein type: Helicase; EC 3.6.4.12
Cellular Component: gamma DNA polymerase complex; mitochondrion; nucleus
Molecular Function: nuclease activity; hydrolase activity; 5'-flap endonuclease activity; metal ion binding; nucleotide binding; iron-sulfur cluster binding; helicase activity; single-stranded DNA-dependent ATPase activity; T/G mismatch-specific endonuclease activity; phosphoric ester hydrolase activity; protein binding; DNA binding; endonuclease activity; 4 iron, 4 sulfur cluster binding; 5'-3' DNA helicase activity; catalytic activity; site-specific endodeoxyribonuclease activity, specific for altered base; ATP binding
Biological Process: mitochondrial DNA repair; DNA replication, removal of RNA primer; metabolic process; DNA double-strand break processing; DNA repair; DNA catabolic process, endonucleolytic; DNA duplex unwinding; DNA replication, Okazaki fragment processing; base-excision repair; mitochondrial DNA replication; DNA replication; DNA replication checkpoint; response to DNA damage stimulus; positive regulation of DNA replication
Research Articles on DNA2
Similar Products
Product Notes
The Mouse DNA2 dna2 (Catalog #AAA9337030) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA9337030 ELISA Kit recognizes Mouse DNA2. It is sometimes possible for the material contained within the vial of "DNA replication helicase 2 homolog (yeast), ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
If you are ready to order, navigate to Shopping Cart and get ready to checkout.
