Mouse MAP-kinase activating death domain ELISA Kit | MADD elisa kit

Mouse MAP kinase-activating death domain protein, MADD ELISA Kit

Cat. Num. AAA9323768

• Gene Names: Madd; IG20; 9630059K23Rik
• Reactivity: Mouse
• Synonyms: MAP-kinase activating death domain; Mouse MAP kinase-activating death domain protein; MADD ELISA Kit; Mouse MAP kinase-activating death domain protein (MADD) ELISA kit; DENN; FLJ35600; FLJ36300; IG20; KIAA0358; RAB3GEP; MAP-kinase activating death domain-containing protein; OTTHUMP00000197132; OTTHUMP00000197133; OTTHUMP00000197138; OTTHUMP00000197139; Rab3 GDP/GTP exch; MADD elisa kit

• Reactivity: Mouse
• Specificity: No significant cross-reactivity or interference between this analyte and analogues is observed.

• Samples: Undiluted original Mouse body fluids, tissue homogenates, secretions or feces samples.
• Assay Type: Sandwich (Quantitative)
• Detection Range: 3.12 ng/ml - 100 ng/ml.
• Sensitivity: 1.0 ng/ml.
• Intra-assay Precision: Intra-assay CV (%) is less than 15%.
• Inter-assay Precision: Inter-assay CV (%) is less than 15%. [CV(%) = SD/mean ×100].
• Preparation and Storage: Store all reagents at 2-8 degree C

Related Product Information for MADD elisa kit

Background: This Quantitative Sandwich ELISA kit is only for in vitro research use only! This kit is intended to be used for determination the level of MADD (hereafter termed "analyte") in undiluted original Mouse body fluids, tissue homogenates, secretions or feces samples. This kit is NOT suitable for assaying non-biological sources of substances.

NCBI and Uniprot Product Information

• NCBI GI #: 295148169
• NCBI GeneID: 228355
• NCBI Accession #: NP_001171190.1
• NCBI GenBank Nucleotide #: NM_001177719.1
• UniProt Accession #: Q80U28; Q3TQK3; Q6P4I6; Q80W52; Q80WL3; Q80WL4; Q80WL5; Q80WL6; Q80WL7; Q80WL8; Q80WL9; Q80WM0
• Molecular Weight: 175,180 Da
• NCBI Official Full Name: MAP kinase-activating death domain protein isoform 10
• NCBI Official Synonym Full Names: MAP-kinase activating death domain
• NCBI Official Symbol: Madd
• NCBI Official Synonym Symbols: IG20; 9630059K23Rik
• NCBI Protein Information: MAP kinase-activating death domain protein; Rab3 GEP; rab3 GDP/GTP exchange factor; Rab3 GDP/GTP exchange protein; insulinoma-glucagonoma protein 20
• UniProt Protein Name: MAP kinase-activating death domain protein
• Protein Family: Malonyl-S-ACP:biotin-protein carboxyltransferase
• UniProt Gene Name: Madd
• UniProt Synonym Gene Names: Kiaa0358
• UniProt Entry Name: MADD_MOUSE

Uniprot Description

MADD: a guanine nucleotide exchange factor (GEF) that activates RAB3A/C/D. A GEF for Rab27A/B in melanocytes and during isoproterenol (IPR)-stimulated amylase release from rat parotid acinar cells. Plays a significant role in regulating cell proliferation, survival and death through alternatively spliced isoforms. Component of the TNFRSF1A signaling complex: MADD links TNFRSF1A with MAP kinase activation. Plays an important regulatory role in physiological cell death (TNF-alpha-induced, caspase-mediated apoptosis). Interacts with the death domain of TNFRSF1A through its own death domain. Highly expressed in fetal brain and kidney, adult testis, ovary, brain and heart. Overexpression of MADD activates the mitogen-activated protein (MAP) kinase extracellular signal-regulated kinase (ERK). Expression of the MADD death domain stimulates both the ERK and c-JUN N-terminal kinase MAP kinases and induces the phosphorylation of cytosolic phospholipase A2. Isoform 1 is pro-apoptotic, isoform 5 is anti-apoptotic and isoform 3 and isoform 4 have no effect. Isoform 5 is constitutively expressed in all tissues. Isoform 7 is expressed in fetal liver and in several cancer cell lines. Isoform 5 is associated with increased cell proliferation and isoform 2 with decreased proliferation. Belongs to the MADD family. Seven isoforms of the human protein are produced by alternative splicing.

Protein type: Membrane protein, integral; GEFs, Rab; Adaptor/scaffold; GEFs

Cellular Component: synaptic vesicle; membrane; cytoplasm; integral to membrane; plasma membrane; synapse; nucleus; cytosol

Molecular Function: guanyl-nucleotide exchange factor activity; Rab guanyl-nucleotide exchange factor activity

Biological Process: regulation of Rab protein signal transduction; negative regulation of growth hormone secretion; regulation of apoptosis; apoptosis; regulation of cell cycle; activation of MAPK activity; positive regulation of GTPase activity