Mouse seven in absentia homolog 2 (Drosophila) ELISA Kit | SIAH2 elisa kit

Mouse E3 ubiquitin-protein ligase SIAH2, SIAH2 ELISA Kit

Cat. Num. AAA9322217

• Gene Names: Siah2; Sinh2; AA415433
• Reactivity: Mouse
• Synonyms: seven in absentia homolog 2 (Drosophila); Mouse E3 ubiquitin-protein ligase SIAH2; SIAH2 ELISA Kit; Mouse E3 ubiquitin-protein ligase SIAH2 (SIAH2) ELISA kit; hSiah2; seven in absentia homolog 2; SIAH2 elisa kit

• Reactivity: Mouse
• Specificity: No significant cross-reactivity or interference between this analyte and analogues is observed.

• Samples: Undiluted original Mouse body fluids, tissue homogenates, secretions or feces samples. This kit is NOT suitable for assaying non-biological sources of substances.
• Assay Type: Sandwich
• Detection Range: 0.625 ng/ml - 20 ng/ml.
• Sensitivity: 0.1ng/ml.
• Intra-assay Precision: Intra-assay CV (%) is less than 15%.
• Inter-assay Precision: Inter-assay CV (%) is less than 15%. [CV(%) = SD/mean ×100].
• Preparation and Storage: Store all reagents at 2-8 degree C

Related Product Information for SIAH2 elisa kit

Background/Introduction: This Quantitative Sandwich ELISA kit is only for in vitro research use only! This kit is intended to be used for determination the level of SIAH2 (hereafter termed "analyte") in undiluted original Mouse body fluids, tissue homogenates, secretions or feces samples. This kit is NOT suitable for assaying non-biological sources of substances.

NCBI and Uniprot Product Information

• NCBI GI #: 40254613
• NCBI GeneID: 20439
• NCBI Accession #: NP_033200.2
• NCBI GenBank Nucleotide #: NM_009174.3
• UniProt Accession #: Q06986
• Molecular Weight: 34,758 Da
• NCBI Official Full Name: E3 ubiquitin-protein ligase SIAH2
• NCBI Official Synonym Full Names: seven in absentia 2
• NCBI Official Symbol: Siah2
• NCBI Official Synonym Symbols: Sinh2; AA415433
• NCBI Protein Information: E3 ubiquitin-protein ligase SIAH2; mSiah2; siah-2; seven in absentia homolog 2
• UniProt Protein Name: E3 ubiquitin-protein ligase SIAH2
• Protein Family: E3 ubiquitin-protein ligase
• UniProt Gene Name: Siah2
• UniProt Synonym Gene Names: Siah-2; mSiah2
• UniProt Entry Name: SIAH2_MOUSE

Uniprot Description

SIAH2: E3 ubiquitin-protein ligase that mediates ubiquitination and subsequent proteasomal degradation of target proteins. E3 ubiquitin ligases accept ubiquitin from an E2 ubiquitin- conjugating enzyme in the form of a thioester and then directly transfers the ubiquitin to targeted substrates. Mediates E3 ubiquitin ligase activity either through direct binding to substrates or by functioning as the essential RING domain subunit of larger E3 complexes. Triggers the ubiquitin-mediated degradation of many substrates, including proteins involved in transcription regulation (POU2AF1, PML, NCOR1), a cell surface receptor (DCC), an antiapoptotic protein (BAG1), and a protein involved in synaptic vesicle function in neurons (SYP). It is thereby involved in apoptosis, tumor suppression, cell cycle, transcription and signaling processes. Has some overlapping function with SIAH1. Triggers the ubiquitin-mediated degradation of TRAF2, whereas SIAH1 can not. Promotes monoubiquitination of SNCA. Homodimer. Interacts with UBE2E2. Interacts with PEG3. Interacts with VAV1, without mediating its ubiquitin- mediated degradation. Interacts with CACYBP/SIP. Probable component of some large E3 complex possibly composed of UBE2D1, SIAH2, CACYBP/SIP, SKP1, APC and TBL1X. Interacts with PEG10, which may inhibit its activity. Interacts with EGLN2 and SNCAIP. Widely expressed at low level. Inhibited by interaction with SNCAIP (isoform 2, but not isoform 1). May be inhibited by interaction with PEG10. Belongs to the SINA (Seven in absentia) family.

Protein type: Ligase; Transcription, coactivator/corepressor; Ubiquitin conjugating system; Ubiquitin ligase; EC 6.3.2.19; EC 6.3.2.-

Cellular Component: nucleoplasm; neuron projection; cell soma; intracellular membrane-bound organelle; cytoplasm; early endosome; cytosol; nucleus

Molecular Function: protein binding; zinc ion binding; ubiquitin conjugating enzyme binding; coenzyme F420-2 alpha-glutamyl ligase activity; UDP-N-acetylmuramoylalanyl-D-glutamyl-2,6-diaminopimelate-D-alanyl-D-alanine ligase activity; metal ion binding; ubiquitin-protein ligase activity; ribosomal S6-glutamic acid ligase activity; coenzyme F420-0 gamma-glutamyl ligase activity; ligase activity

Biological Process: ubiquitin-dependent protein catabolic process; apoptosis; regulation of protein ubiquitination; cellular protein catabolic process; multicellular organismal development; protein ubiquitination; negative regulation of caspase activity; cell cycle; negative regulation of apoptosis